Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,273,062 C→G 14.6% intergenic (+27/+264) PP_2003 → / ← PP_2004 hypothetical protein/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,273,0620CG14.6% 127.0 / 13.1 48intergenic (+27/+264)PP_2003/PP_2004hypothetical protein/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base G (3/4);  total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

CAGCGGCGCCGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTA  >  NC_002947/2272914‑2273209
                                                                                                                                                     |                                                                                                                                                   
cAGCGGCGCCGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGcc                                                                                                                                                     <  5:56352/149‑1 (MQ=255)
  gcggcgCCGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCgg                                                                                                                                                   <  6:48814/149‑1 (MQ=255)
         cGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCcg                                                                                                                                            <  7:90195/149‑1 (MQ=255)
                   tGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAgggtc                                                                                                                                  <  2:312662/149‑3 (MQ=255)
                     aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCgg                                                                                                                                <  3:37208/149‑1 (MQ=255)
                     aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCgg                                                                                                                                <  5:316043/149‑1 (MQ=255)
                     aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTAGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCg                                                                                                                                 <  5:373162/148‑1 (MQ=38)
                       aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCCACCCATTAGCCGGCAGCCCGCGCAACGGCCGGt                                                                                                                               >  2:167159/1‑148 (MQ=255)
                           aGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAg                                                                                                                           <  3:311054/148‑1 (MQ=38)
                           aGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAg                                                                                                                           >  4:311054/1‑148 (MQ=38)
                           aGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAgg                                                                                                                          <  8:279367/149‑1 (MQ=255)
                                 cGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTa                                                                                                                     >  8:217323/1‑148 (MQ=255)
                                    aGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACa                                                                                                                 >  6:93679/1‑149 (MQ=255)
                                          tgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATcccc                                                                                                            <  5:26573/148‑1 (MQ=255)
                                                      ccgAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCgg                                                                                                                                                   <  5:365026/97‑1 (MQ=255)
                                                      ccgAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCgg                                                                                                                                                   >  6:365026/1‑97 (MQ=255)
                                                            cTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTGCAGACGCCCCGCGa                                                                                         >  8:250594/1‑149 (MQ=255)
                                                             tGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGa                                                                                         >  1:260084/1‑149 (MQ=255)
                                                                        aGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTGCAGACGCCCCGCGATAACTTTCTGaa                                                                             <  7:250594/149‑1 (MQ=255)
                                                                           tGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAacac                                                                          <  4:294189/149‑1 (MQ=255)
                                                                               gcagGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGc                                                                      <  7:217323/149‑1 (MQ=255)
                                                                                        ccAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGATAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc                                                             >  4:71446/1‑149 (MQ=255)
                                                                                        ccAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc                                                             <  1:162672/149‑1 (MQ=255)
                                                                                        ccAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc                                                             <  1:335865/149‑1 (MQ=255)
                                                                                        ccAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCATTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACCCCCCGCGATAACTTTCTGAACACCAGCGACGTTTAc                                                             >  4:314231/1‑149 (MQ=255)
                                                                                                    ggCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCt                                                 <  2:260084/149‑1 (MQ=255)
                                                                                                     gcc‑gccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCtt                                                <  1:167159/149‑1 (MQ=255)
                                                                                                       c‑gccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCGTTAGCCGGCAGCCCGCGAAAGGGCCGGGTCAGGGTGTAAACATCCCCAGGTTCAGACGCCCCGCGAGACCTTTGTGAACACCTGGGGCGGTTTCCACTCAGAAGCGTg                                               >  2:298101/1‑148 (MQ=255)
                                                                                                         gccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCAc                                                          >  5:305943/1‑136 (MQ=255)
                                                                                                         gccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCAc                                                          <  6:305943/136‑1 (MQ=255)
                                                                                                          ccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtca                                            <  5:93679/149‑1 (MQ=255)
                                                                                                             cccccTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGTAAGGGCCGGTTCAGGCTGTAAAGATCCCCTGTTTCAGTCGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtcatca                                         <  3:188302/149‑1 (MQ=255)
                                                                                                                 ctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATccc                                     >  5:178261/1‑149 (MQ=255)
                                                                                                                   ccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCtt                                   >  1:196235/1‑149 (MQ=255)
                                                                                                                     ctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCt                                                                                <  7:314932/102‑1 (MQ=255)
                                                                                                                     ctTTGATGGGTCGACTGAACCGACCCTTTCGCCGGCAGCAAGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTTCAGACGCCCCGCGATAACTTTCAGAACCACGGATACGTTTACCACTCATAAGATTCTCATCATCCCTTc                                  >  2:235664/1‑148 (MQ=255)
                                                                                                                      tttGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCAt                                >  7:95371/1‑149 (MQ=255)
                                                                                                                              gTCGCCTGAACCGCCCCATTAGCCGGCCGCCCGCGAAAGGGCCGGTTCAGGCTGTTAACATACCCAGTTAAAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTTCCACGCATAAGCTTGtaa                                            >  4:77563/1‑127 (MQ=255)
                                                                                                                              gTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtca                                            <  3:77563/129‑1 (MQ=255)
                                                                                                                                  ccTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCt                      >  7:339620/1‑147 (MQ=255)
                                                                                                                                  ccTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTa                    <  6:178261/149‑1 (MQ=255)
                                                                                                                                   cTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAg                   <  3:338313/149‑1 (MQ=255)
                                                                                                                                     gAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGc                 >  7:12886/1‑149 (MQ=255)
                                                                                                                                         cggcccTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTg               >  8:298952/4‑147 (MQ=39)
                                                                                                                                           aCCCTTTCGCCGGCAGCCCGCGATAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAgg           <  3:71446/149‑1 (MQ=255)
                                                                                                                                              cTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa        >  7:53816/1‑149 (MQ=255)
                                                                                                                                              cTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa        <  8:53816/149‑1 (MQ=255)
                                                                                                                                              cTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATAATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa        >  8:124391/1‑149 (MQ=255)
                                                                                                                                                   gCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCt   <  2:196235/149‑1 (MQ=255)
                                                                                                                                                     cGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACACCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACGCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTa  >  3:291601/1‑148 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CAGCGGCGCCGGTGGTGGATGACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTA  >  NC_002947/2272914‑2273209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑