Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,273,080 C→T 12.2% intergenic (+45/+246) PP_2003 → / ← PP_2004 hypothetical protein/AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,273,0800CT12.2% 123.4 / 8.3 49intergenic (+45/+246)PP_2003/PP_2004hypothetical protein/AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (22/21);  new base T (3/3);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

ACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAGCA  >  NC_002947/2272935‑2273226
                                                                                                                                                  |                                                                                                                                                  
aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCgg                                                                                                                                                 <  5:316043/149‑1 (MQ=255)
aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCgg                                                                                                                                                 <  3:37208/149‑1 (MQ=255)
aCAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTAGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCg                                                                                                                                                  <  5:373162/148‑1 (MQ=38)
  aGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCCACCCATTAGCCGGCAGCCCGCGCAACGGCCGGt                                                                                                                                                >  2:167159/1‑148 (MQ=255)
      aGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAg                                                                                                                                            >  4:311054/1‑148 (MQ=38)
      aGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGGCCCTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAg                                                                                                                                            <  3:311054/148‑1 (MQ=38)
      aGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAgg                                                                                                                                           <  8:279367/149‑1 (MQ=255)
            cGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTa                                                                                                                                      >  8:217323/1‑148 (MQ=255)
               aGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACa                                                                                                                                  >  6:93679/1‑149 (MQ=255)
                     tgGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATcccc                                                                                                                             <  5:26573/148‑1 (MQ=255)
                                       cTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTGCAGACGCCCCGCGa                                                                                                          >  8:250594/1‑149 (MQ=255)
                                        tGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCCGGCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGa                                                                                                          >  1:260084/1‑149 (MQ=255)
                                                   aGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTGCAGACGCCCCGCGATAACTTTCTGaa                                                                                              <  7:250594/149‑1 (MQ=255)
                                                      tGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAacac                                                                                           <  4:294189/149‑1 (MQ=255)
                                                          gcagGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGc                                                                                       <  7:217323/149‑1 (MQ=255)
                                                                   ccAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGATAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc                                                                              >  4:71446/1‑149 (MQ=255)
                                                                   ccAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc                                                                              <  1:162672/149‑1 (MQ=255)
                                                                   ccAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTAc                                                                              <  1:335865/149‑1 (MQ=255)
                                                                   ccAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCATTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACCCCCCGCGATAACTTTCTGAACACCAGCGACGTTTAc                                                                              >  4:314231/1‑149 (MQ=255)
                                                                               ggCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCt                                                                  <  2:260084/149‑1 (MQ=255)
                                                                                gcc‑gccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCtt                                                                 <  1:167159/149‑1 (MQ=255)
                                                                                  c‑gccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCGTTAGCCGGCAGCCCGCGAAAGGGCCGGGTCAGGGTGTAAACATCCCCAGGTTCAGACGCCCCGCGAGACCTTTGTGAACACCTGGGGCGGTTTCCACTCAGAAGCGTg                                                                >  2:298101/1‑148 (MQ=255)
                                                                                    gccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCAc                                                                           <  6:305943/136‑1 (MQ=255)
                                                                                    gccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCAc                                                                           >  5:305943/1‑136 (MQ=255)
                                                                                     ccgccCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtca                                                             <  5:93679/149‑1 (MQ=255)
                                                                                        cccccTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGTAAGGGCCGGTTCAGGCTGTAAAGATCCCCTGTTTCAGTCGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtcatca                                                          <  3:188302/149‑1 (MQ=255)
                                                                                            ctccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATccc                                                      >  5:178261/1‑149 (MQ=255)
                                                                                              ccctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCtt                                                    >  1:196235/1‑149 (MQ=255)
                                                                                                ctTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCt                                                                                                 <  7:314932/102‑1 (MQ=255)
                                                                                                ctTTGATGGGTCGACTGAACCGACCCTTTCGCCGGCAGCAAGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTTCAGACGCCCCGCGATAACTTTCAGAACCACGGATACGTTTACCACTCATAAGATTCTCATCATCCCTTc                                                   >  2:235664/1‑148 (MQ=255)
                                                                                                 tttGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCAt                                                 >  7:95371/1‑149 (MQ=255)
                                                                                                         gTCGCCTGAACCGCCCCATTAGCCGGCCGCCCGCGAAAGGGCCGGTTCAGGCTGTTAACATACCCAGTTAAAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTTCCACGCATAAGCTTGtaa                                                             >  4:77563/1‑127 (MQ=255)
                                                                                                         gTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGtca                                                             <  3:77563/129‑1 (MQ=255)
                                                                                                             ccTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCt                                       >  7:339620/1‑147 (MQ=255)
                                                                                                             ccTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTa                                     <  6:178261/149‑1 (MQ=255)
                                                                                                              cTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAg                                    <  3:338313/149‑1 (MQ=255)
                                                                                                                gAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGc                                  >  7:12886/1‑149 (MQ=255)
                                                                                                                    cggcccTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTg                                >  8:298952/4‑147 (MQ=39)
                                                                                                                      aCCCTTTCGCCGGCAGCCCGCGATAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAgg                            <  3:71446/149‑1 (MQ=255)
                                                                                                                         cTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa                         >  7:53816/1‑149 (MQ=255)
                                                                                                                         cTTTCGCGGGCTGCCGGCGAAAGGGTCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa                         <  8:53816/149‑1 (MQ=255)
                                                                                                                         cTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATAATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGa                         >  8:124391/1‑149 (MQ=255)
                                                                                                                              gCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCt                    <  2:196235/149‑1 (MQ=255)
                                                                                                                                cGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACACCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACGCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTa                   >  3:291601/1‑148 (MQ=255)
                                                                                                                                       ccGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGc            >  3:149661/1‑148 (MQ=255)
                                                                                                                                        cgcgAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCa           >  5:1488/1‑148 (MQ=255)
                                                                                                                                                 gCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGaa                              >  4:233759/1‑120 (MQ=255)
                                                                                                                                                 gCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGaa                              <  3:233759/120‑1 (MQ=255)
                                                                                                                                                 gCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAGCa  >  6:167053/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACAGCCAGGGTTCGCAGCCTGTGGCCCCGCCGGCCGAAGCTGCACCCATTCAGGTGCAGCAGGATGCCCAGCCGGCCGAGGCC‑GCCGCCCCCTCCCTTTGATGGGTCGCCTGAACCGACCCTTTCGCCGGCAGCCCGCGAAAGGGCCGGTTCAGGCTGTAAACATCCCCTGTTACAGACGCCCCGCGATAACTTTCTGAACACCTGCTACGTTTACCACTCATAAGCTTGTCATCATCCCTTCATCCCACCTGCTTAGGCTGAAGGCGATACCTACAAAGGCAAAATGAGCA  >  NC_002947/2272935‑2273226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑