Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,305,212 G→A 11.6% L449L (CTG→TTG)  PP_2028 ← TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,305,2120GA11.6% 184.7 / 12.6 70L449L (CTG→TTG) PP_2028TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (33/28);  new base A (5/3);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CGGGGCTGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGT  >  NC_002947/2305065‑2305355
                                                                                                                                                   |                                                                                                                                               
cGGGGCTGGACTGTTGGTCGGCGCCGGGGGTGCCGTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTGCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGcagcag                                                                                                                                                 <  4:219156/148‑1 (MQ=255)
    gCTGGCCTGTTGGTCGTCGCCGGGTGTTCCTTGGGCCGGGGTGCTGGCGGAGCTGCTGTTGGCTTCGGTTTCGCTGGCGGGGGTGTCTTGGGCTTCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgct                                                                                                                                            <  5:18844/149‑1 (MQ=255)
        gCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                        >  6:145231/1‑149 (MQ=255)
        gCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGACTTCACGTTGTTGCAGCAGTtgctgc                                                                                                                                          <  3:133034/147‑1 (MQ=255)
         ccTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTACCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                        >  4:166958/1‑148 (MQ=255)
         ccTGTTGGTCGTCGCCGGGTGTGCCTTGCGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                        >  1:208813/1‑148 (MQ=255)
          cTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                        >  3:25898/1‑147 (MQ=255)
               ggTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgctg                                                                                                                                           <  5:328391/139‑1 (MQ=255)
               ggTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgctg                                                                                                                                           >  6:328391/1‑139 (MQ=255)
                gtcgtcGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCt                                                                                                                                >  7:141475/1‑149 (MQ=255)
                  cgtcgCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAACTGCTGCAACAacgtgaag                                                                                                                              <  3:144742/149‑6 (MQ=255)
                        cGGGGGGGCCTTGGGCCGGGCTGCTGGCCGAGGTGCTGGTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTcc                                                                                                                        <  1:141280/149‑1 (MQ=255)
                         gggTGTGGCTTGGGCCGGGCTGCTGGCGGAGGTGGTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTGACGTTGTTGCTGCAGTTGCTGCACCTGCGCCTGGTTGTCCa                                                                                                                       <  5:176162/149‑1 (MQ=255)
                             gtgCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAcc                                                                                                                                       <  6:83806/129‑1 (MQ=255)
                             gtgCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAcc                                                                                                                                       >  5:83806/1‑129 (MQ=255)
                                 cTTGGGCCGGGCTGCTGGCCGTGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGcc                                                                                                               >  4:357535/1‑149 (MQ=255)
                                  ttGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCt                                                                                                              >  7:63050/1‑149 (MQ=255)
                                            ctgctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCggg                                                                                                    >  5:137034/1‑149 (MQ=255)
                                             tgctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGc                                                                                                   >  5:213227/1‑149 (MQ=255)
                                              gctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCt                                                                                                  >  8:30904/1‑149 (MQ=255)
                                               ccggCCGAGCGGCGGGTGCCGTCGGCCTCGCTGCCGGGCGTGGCTTGGGCAGCGCTGCCGGGCGGTTGTCCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTCGTTGTCCAGCCCGACCTGCAGGCCgcgc                                                                                                   <  4:25898/145‑1 (MQ=255)
                                                tgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGa                                                                                                >  6:337946/1‑149 (MQ=255)
                                                 ggCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGATGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGAc                                                                                               >  6:265211/1‑149 (MQ=255)
                                                  gCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACg                                                                                              >  8:79392/1‑149 (MQ=255)
                                                          gctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGa                                                                                                <  2:171618/139‑1 (MQ=255)
                                                          gctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGa                                                                                                >  1:171618/1‑139 (MQ=255)
                                                           ctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGc                                                                                     <  7:154232/149‑1 (MQ=255)
                                                              ttGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCtt                                                                                   <  2:6097/148‑1 (MQ=255)
                                                                gCCTTCGTTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGGGGTTGTTCCTCGGCCGTGGCTTCTCGTTGTTGGAGCTGTTGCTGGACGAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGc                                                                                 <  7:120117/148‑1 (MQ=255)
                                                                 ccTTCGGTTTCGCTGCCGGGGGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTc                                                                               <  8:141475/149‑1 (MQ=255)
                                                                 ccTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCGGACGCTCCAGGGCTTGATc                                                                               >  6:191235/1‑149 (MQ=255)
                                                                  cTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCg                                                                              >  3:14567/1‑149 (MQ=255)
                                                                   ttCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCg                                                                              >  3:221418/1‑148 (MQ=255)
                                                                     cGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGt                                                                             <  5:337946/147‑1 (MQ=255)
                                                                     cGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTa                                                                            <  7:303627/148‑1 (MQ=255)
                                                                      ggTTTCGGTGCCTGGCGGGGCTTGGGCATCGCTGCCGGGCGGTTGTGCCTCGGCCTTGGCTTCAGGTTGTTGCAGCAGTTGCTGCAGCAGCGCCGGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAg                                                                           <  6:243110/148‑1 (MQ=255)
                                                                      ggTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAgg                                                                          >  4:211199/1‑149 (MQ=255)
                                                                      ggTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAgg                                                                          >  6:101768/1‑149 (MQ=255)
                                                                      ggTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAg                                                                           <  4:221418/148‑1 (MQ=255)
                                                                          tCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATc                                                                      >  4:152719/1‑149 (MQ=255)
                                                                           cGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGATCCTCGGCCTTGTCTTCACGTTGTTGCAGCACTTGATGCACCAGCGCCTGGATGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATc                                                                      >  7:250906/1‑148 (MQ=255)
                                                                                   ggCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGggcggc                                                             <  6:213227/149‑1 (MQ=255)
                                                                                      gtgtCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTc                                                          >  3:266636/1‑149 (MQ=255)
                                                                                             gggCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGCTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCt                                                     <  5:265211/147‑1 (MQ=255)
                                                                                                      tGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCgg                                          <  4:14567/149‑1 (MQ=255)
                                                                                                            gCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAgg                                    <  6:137034/149‑1 (MQ=255)
                                                                                                             cGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAggg                                   >  3:23405/1‑149 (MQ=255)
                                                                                                               gttgttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCa                                 >  8:98347/1‑149 (MQ=255)
                                                                                                                  gttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTg                              >  3:76359/1‑149 (MQ=255)
                                                                                                                   ttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAg                                                       <  3:40549/123‑1 (MQ=255)
                                                                                                                   ttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAg                                                       >  4:40549/1‑123 (MQ=255)
                                                                                                                               ggCTTCACGTTGTGGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTa                   <  5:145231/147‑1 (MQ=255)
                                                                                                                                gCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGt                 <  7:30904/148‑1 (MQ=255)
                                                                                                                                gCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTg                <  2:208813/149‑1 (MQ=255)
                                                                                                                                gCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTg                <  3:357535/149‑1 (MQ=255)
                                                                                                                                gCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTg                <  3:166958/149‑1 (MQ=255)
                                                                                                                                 cTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTgg               >  4:191271/1‑149 (MQ=255)
                                                                                                                                  ttCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTggg              >  2:89955/1‑149 (MQ=255)
                                                                                                                                    cACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGgc             <  4:76359/148‑1 (MQ=255)
                                                                                                                                     aCGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGgctg           >  3:127082/1‑149 (MQ=255)
                                                                                                                                       gttgttGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGgctgct         >  6:23385/1‑149 (MQ=255)
                                                                                                                                       gctggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGt                 >  8:114965/6‑141 (MQ=255)
                                                                                                                                       gctggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGt                 <  7:114965/136‑1 (MQ=255)
                                                                                                                                       gctggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGgctgct         >  2:273282/6‑149 (MQ=255)
                                                                                                                                         tggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGctgctg        >  6:183956/4‑148 (MQ=255)
                                                                                                                                         tggtgCAGCAACTGCTGCAACAACGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGctgctg        >  6:65599/4‑148 (MQ=255)
                                                                                                                                          gttgCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCGTCGATTGTAGTGGGctgctg        >  2:90615/1‑147 (MQ=255)
                                                                                                                                           gtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAg     >  6:135171/2‑149 (MQ=255)
                                                                                                                                            tgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGGTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAgt    <  5:135171/149‑1 (MQ=255)
                                                                                                                                             gcagcaGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTcc                                                                                          <  7:92089/62‑1 (MQ=255)
                                                                                                                                             gcagcaGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTcc                                                                                          >  8:92089/1‑62 (MQ=255)
                                                                                                                                              cagcagTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAgtgt  >  3:366523/1‑149 (MQ=255)
                                                                                                                                              cagcagTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAgtgt  >  1:340663/1‑149 (MQ=255)
                                                                                                                                              cagcagTTGCTGCACAAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAgtgt  >  1:335997/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CGGGGCTGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGT  >  NC_002947/2305065‑2305355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑