Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,305,213 T→C 10.9% Q448Q (CAA→CAG)  PP_2028 ← TPR repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,305,2130TC10.9% 194.7 / 12.1 73Q448Q (CAA→CAG) PP_2028TPR repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (37/28);  new base C (5/3);  total (42/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGT  >  NC_002947/2305069‑2305355
                                                                                                                                                |                                                                                                                                              
gCTGGCCTGTTGGTCGTCGCCGGGTGTTCCTTGGGCCGGGGTGCTGGCGGAGCTGCTGTTGGCTTCGGTTTCGCTGGCGGGGGTGTCTTGGGCTTCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgct                                                                                                                                            <  5:18844/149‑1 (MQ=255)
    gCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                        >  6:145231/1‑149 (MQ=255)
    gCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGACTTCACGTTGTTGCAGCAGTtgctgc                                                                                                                                          <  3:133034/147‑1 (MQ=255)
     ccTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTACCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                        >  4:166958/1‑148 (MQ=255)
     ccTGTTGGTCGTCGCCGGGTGTGCCTTGCGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                        >  1:208813/1‑148 (MQ=255)
      cTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAc                                                                                                                                        >  3:25898/1‑147 (MQ=255)
           ggTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgctg                                                                                                                                           >  6:328391/1‑139 (MQ=255)
           ggTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTtgctg                                                                                                                                           <  5:328391/139‑1 (MQ=255)
            gtcgtcGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCt                                                                                                                                >  7:141475/1‑149 (MQ=255)
              cgtcgCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAACTGCTGCAACAacgtgaag                                                                                                                              <  3:144742/149‑6 (MQ=255)
                    cGGGGGGGCCTTGGGCCGGGCTGCTGGCCGAGGTGCTGGTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTcc                                                                                                                        <  1:141280/149‑1 (MQ=255)
                     gggTGTGGCTTGGGCCGGGCTGCTGGCGGAGGTGGTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTGACGTTGTTGCTGCAGTTGCTGCACCTGCGCCTGGTTGTCCa                                                                                                                       <  5:176162/149‑1 (MQ=255)
                         gtgCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAcc                                                                                                                                       <  6:83806/129‑1 (MQ=255)
                         gtgCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCAcc                                                                                                                                       >  5:83806/1‑129 (MQ=255)
                             cTTGGGCCGGGCTGCTGGCCGTGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGcc                                                                                                               >  4:357535/1‑149 (MQ=255)
                              ttGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCt                                                                                                              >  7:63050/1‑149 (MQ=255)
                                        ctgctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCggg                                                                                                    >  5:137034/1‑149 (MQ=255)
                                         tgctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGc                                                                                                   >  5:213227/1‑149 (MQ=255)
                                          gctgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCt                                                                                                  >  8:30904/1‑149 (MQ=255)
                                           ccggCCGAGCGGCGGGTGCCGTCGGCCTCGCTGCCGGGCGTGGCTTGGGCAGCGCTGCCGGGCGGTTGTCCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTCGTTGTCCAGCCCGACCTGCAGGCCgcgc                                                                                                   <  4:25898/145‑1 (MQ=255)
                                            tgGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGa                                                                                                >  6:337946/1‑149 (MQ=255)
                                             ggCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGATGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGAc                                                                                               >  6:265211/1‑149 (MQ=255)
                                              gCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACg                                                                                              >  8:79392/1‑149 (MQ=255)
                                                      gctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGa                                                                                                >  1:171618/1‑139 (MQ=255)
                                                      gctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGa                                                                                                <  2:171618/139‑1 (MQ=255)
                                                       ctgTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGc                                                                                     <  7:154232/149‑1 (MQ=255)
                                                          ttGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCtt                                                                                   <  2:6097/148‑1 (MQ=255)
                                                            gCCTTCGTTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGGGGTTGTTCCTCGGCCGTGGCTTCTCGTTGTTGGAGCTGTTGCTGGACGAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGc                                                                                 <  7:120117/148‑1 (MQ=255)
                                                             ccTTCGGTTTCGCTGCCGGGGGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTc                                                                               <  8:141475/149‑1 (MQ=255)
                                                             ccTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCGGACGCTCCAGGGCTTGATc                                                                               >  6:191235/1‑149 (MQ=255)
                                                              cTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCg                                                                              >  3:14567/1‑149 (MQ=255)
                                                               ttCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCg                                                                              >  3:221418/1‑148 (MQ=255)
                                                                 cGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGt                                                                             <  5:337946/147‑1 (MQ=255)
                                                                 cGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTa                                                                            <  7:303627/148‑1 (MQ=255)
                                                                  ggTTTCGGTGCCTGGCGGGGCTTGGGCATCGCTGCCGGGCGGTTGTGCCTCGGCCTTGGCTTCAGGTTGTTGCAGCAGTTGCTGCAGCAGCGCCGGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAg                                                                           <  6:243110/148‑1 (MQ=255)
                                                                  ggTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAgg                                                                          >  6:101768/1‑149 (MQ=255)
                                                                  ggTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAgg                                                                          >  4:211199/1‑149 (MQ=255)
                                                                  ggTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAg                                                                           <  4:221418/148‑1 (MQ=255)
                                                                      tCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATc                                                                      >  4:152719/1‑149 (MQ=255)
                                                                       cGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGATCCTCGGCCTTGTCTTCACGTTGTTGCAGCACTTGATGCACCAGCGCCTGGATGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATc                                                                      >  7:250906/1‑148 (MQ=255)
                                                                               ggCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGggcggc                                                             <  6:213227/149‑1 (MQ=255)
                                                                                  gtgtCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTc                                                          >  3:266636/1‑149 (MQ=255)
                                                                                         gggCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGCTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCt                                                     <  5:265211/147‑1 (MQ=255)
                                                                                                  tGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCgg                                          <  4:14567/149‑1 (MQ=255)
                                                                                                        gCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAgg                                    <  6:137034/149‑1 (MQ=255)
                                                                                                         cGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAggg                                   >  3:23405/1‑149 (MQ=255)
                                                                                                           gttgttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCa                                 >  8:98347/1‑149 (MQ=255)
                                                                                                              gttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTg                              >  3:76359/1‑149 (MQ=255)
                                                                                                               ttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAg                                                       <  3:40549/123‑1 (MQ=255)
                                                                                                               ttCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAg                                                       >  4:40549/1‑123 (MQ=255)
                                                                                                                           ggCTTCACGTTGTGGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTa                   <  5:145231/147‑1 (MQ=255)
                                                                                                                            gCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGt                 <  7:30904/148‑1 (MQ=255)
                                                                                                                            gCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTg                <  2:208813/149‑1 (MQ=255)
                                                                                                                            gCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTg                <  3:357535/149‑1 (MQ=255)
                                                                                                                            gCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTg                <  3:166958/149‑1 (MQ=255)
                                                                                                                             cTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTgg               >  4:191271/1‑149 (MQ=255)
                                                                                                                              ttCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTggg              >  2:89955/1‑149 (MQ=255)
                                                                                                                                cACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGgc             <  4:76359/148‑1 (MQ=255)
                                                                                                                                 aCGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGgctg           >  3:127082/1‑149 (MQ=255)
                                                                                                                                   gttgttGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGgctgct         >  6:23385/1‑149 (MQ=255)
                                                                                                                                   gctggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGt                 >  8:114965/6‑141 (MQ=255)
                                                                                                                                   gctggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGt                 <  7:114965/136‑1 (MQ=255)
                                                                                                                                   gctggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGgctgct         >  2:273282/6‑149 (MQ=255)
                                                                                                                                     tggtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGctgctg        >  6:183956/4‑148 (MQ=255)
                                                                                                                                     tggtgCAGCAACTGCTGCAACAACGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGctgctg        >  6:65599/4‑148 (MQ=255)
                                                                                                                                      gttgCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCGTCGATTGTAGTGGGctgctg        >  2:90615/1‑147 (MQ=255)
                                                                                                                                       gtgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAg     >  6:135171/2‑149 (MQ=255)
                                                                                                                                        tgCAGCAACTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGGTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAgt    <  5:135171/149‑1 (MQ=255)
                                                                                                                                         gcagcaGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTcc                                                                                          <  7:92089/62‑1 (MQ=255)
                                                                                                                                         gcagcaGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTcc                                                                                          >  8:92089/1‑62 (MQ=255)
                                                                                                                                          cagcagTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAgtgt  >  3:366523/1‑149 (MQ=255)
                                                                                                                                          cagcagTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAgtgt  >  1:340663/1‑149 (MQ=255)
                                                                                                                                          cagcagTTGCTGCACAAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAgtgt  >  1:335997/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                              
GCTGGCCTGTTGGTCGTCGCCGGGTGTGCCTTGGGCCGGGCTGCTGGCCGAGCTGCTGTTGCCTTCGGTTTCGCTGCCGGGCGTGTCTTGGGCATCGCTGCCGGGCGGTTGTTCCTCGGCCTTGGCTTCACGTTGTTGCAGCAGTTGCTGCACCAGCGCCTGGTTGTCCAGCGCGGCCTGCAGGTCGGGCTGACGCTCCAGGGCTTGCTCGTAGGCATCCAGGGCGGCTTCCAGCTCGCCACTGCGGGCCAGGGCATTGCCTCGATTGTAGTGGGCTGCTGCAGTGT  >  NC_002947/2305069‑2305355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑