Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,338,785 A→G 8.1% D193G (GAC→GGC)  PP_2055 → isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,338,7850AG 8.1% 133.7 / 3.1 49D193G (GAC→GGC) PP_2055isomerase
Reads supporting (aligned to +/- strand):  ref base A (21/24);  new base G (2/2);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

GAAGGCCGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGAT  >  NC_002947/2338637‑2338933
                                                                                                                                                    |                                                                                                                                                    
gAAGGCCGCACGGCGATCGTTGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGa                                                                                                                                                      >  1:244959/1‑149 (MQ=255)
   ggCCGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACAt                                                                                                                                                   >  4:208724/1‑149 (MQ=255)
    gCCGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATg                                                                                                                                                  <  1:19516/149‑1 (MQ=255)
    gCCGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATg                                                                                                                                                  <  3:208724/149‑1 (MQ=255)
    gCCGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATg                                                                                                                                                  <  6:25255/149‑1 (MQ=255)
     ccGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATgg                                                                                                                                                 <  3:111445/149‑1 (MQ=255)
          cGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATg                                                                                                                                            <  3:11853/149‑1 (MQ=255)
           ggCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGc                                                                                                                                           >  1:152309/1‑149 (MQ=255)
              gatcgatGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCga                                                                                                                                        <  7:269998/149‑1 (MQ=255)
                  gatGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGGCATGGCCATGTCgatgc                                                                                                                                     <  5:250246/148‑2 (MQ=255)
                    tGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATgg                                                                                                                                  >  7:204691/1‑149 (MQ=255)
                      gggTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGGCATGGCCATGTCgatgcag                                                                                                                                   <  3:2139/146‑4 (MQ=255)
                      gggTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGGCATGGCCATGTCgatgcag                                                                                                                                   >  4:2139/1‑143 (MQ=255)
                           ccGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTgg                                                                                                                            <  5:69467/148‑1 (MQ=255)
                            cGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAg                                                                                                                          <  8:252478/149‑1 (MQ=255)
                              ggCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGgc                                                                                                                        >  1:41353/1‑149 (MQ=255)
                                        ccccGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTg                                                                                                              <  3:329215/149‑1 (MQ=255)
                                           cGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGgt                                                                                                           <  2:152309/149‑1 (MQ=255)
                                                             tCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAAcc                                                                                         >  3:142091/1‑149 (MQ=255)
                                                                aCGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCg                                                                                      <  2:322851/149‑1 (MQ=255)
                                                                 cgcgGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACAAGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCgg                                                                                     <  6:329966/149‑1 (MQ=255)
                                                                         gcagcaAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTg                                                                             >  4:213558/1‑149 (MQ=255)
                                                                                 aCCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCaa                                                                     <  3:213558/149‑1 (MQ=255)
                                                                                   cGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCaa                                                                     >  3:31896/1‑147 (MQ=255)
                                                                                   cGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCaa                                                                     >  1:224795/1‑147 (MQ=255)
                                                                                   cGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACa                                                                   <  5:201311/149‑1 (MQ=255)
                                                                                     gCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAgc                                                                 <  5:239628/149‑1 (MQ=255)
                                                                                                 ccACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGCCGCCCACAGCGCGCTGCTCGaa                                                     >  7:115374/1‑149 (MQ=255)
                                                                                                 ccACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                     >  8:215116/1‑149 (MQ=255)
                                                                                                  cACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAAc                                                    <  8:204691/149‑1 (MQ=255)
                                                                                                  cACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAAc                                                    >  8:202893/1‑149 (MQ=255)
                                                                                                    cGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGc                                                  <  2:244959/149‑1 (MQ=255)
                                                                                                       gCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGACACCCCGGCTCAGCTGGCCGCCACCCGCGCGCTGCTCGAACGCCTg                                               >  2:314458/1‑149 (MQ=255)
                                                                                                       gCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGc                                                                        >  8:99941/1‑124 (MQ=255)
                                                                                                       gCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGc                                                                        <  7:99941/124‑1 (MQ=255)
                                                                                                         aaGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTgg                                              >  4:18302/1‑148 (MQ=255)
                                                                                                              ccAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                     >  6:96597/1‑136 (MQ=255)
                                                                                                              ccAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGaa                                                     <  5:96597/136‑1 (MQ=255)
                                                                                                                     gTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCt                                 >  1:300081/1‑149 (MQ=255)
                                                                                                                             tGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGc                                          >  4:255515/1‑132 (MQ=255)
                                                                                                                             tGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGc                                          <  3:255515/132‑1 (MQ=255)
                                                                                                                                   gCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAgg                   >  6:270878/1‑149 (MQ=255)
                                                                                                                                     cGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGcc                 <  4:142091/149‑1 (MQ=255)
                                                                                                                                          aCCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCTGAAACCCCGGCTCAGCTGGACGCCAACAGAGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATgg             <  4:31896/148‑1 (MQ=255)
                                                                                                                                            cTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGcc          >  3:219405/1‑149 (MQ=255)
                                                                                                                                              tcATCGGCATGGCCATGTCGATGATGGTTGTGGAGGCCAGCCAGTTGGGAGTCAGCCGCTCGGAACCCCAGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGACCGCCTGGAAGCGTTGCGCCTGAAGCCCGGAAAGGCCATGGGCCTg        >  7:268367/2‑149 (MQ=255)
                                                                                                                                               cATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTgg       >  4:246360/1‑149 (MQ=255)
                                                                                                                                                aTCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTggg      <  3:305057/149‑1 (MQ=255)
                                                                                                                                                 tCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGc     >  8:163457/1‑149 (MQ=255)
                                                                                                                                                   gACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGa   <  2:41353/149‑1 (MQ=255)
                                                                                                                                                    aCATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGAt  >  8:42352/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAGGCCGCACGGCGATCGATGGGGTACCGGGCACTGCCGCCCCGGTGCATCTGACCTTCCTCGACGCGGTCGGCAGCAAGACCGGCAAGCTGTTCCCCACGGGCAAGGCCCAGGACGTGATTGATGGCGTGCCGGTGACCTGCATCGACATGGCCATGCCGATGATGGTTGTGGAGGCCAGCCAGTTGGGTGTCAGCGGCTCGGAAACCCCGGCTCAGCTGGACGCCAACAGCGCGCTGCTCGAACGCCTGGAAGCGTTGCGGCTGAAGGCCGGAAAGGCCATGGGCCTGGGCGAT  >  NC_002947/2338637‑2338933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑