Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,367,411 G→T 9.2% V77L (GTG→TTG)  gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,367,4110GT 9.2% 221.6 / 7.7 74V77L (GTG→TTG) gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (31/35);  new base T (3/4);  total (35/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAAAC  >  NC_002947/2367266‑2367559
                                                                                                                                                 |                                                                                                                                                    
ccTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGTACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGc                                                                                                                                                   <  8:270529/149‑1 (MQ=255)
ccTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTg                                                                                                                                                    <  3:7398/148‑1 (MQ=255)
 cTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCg                                                                                                                                                  >  5:175441/1‑149 (MQ=255)
  tGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCgg                                                                                                                                                 >  2:204766/1‑149 (MQ=255)
    ccATAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCgg                                                                                                                                                 <  5:199211/147‑1 (MQ=255)
    ccACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCggg                                                                                                                                                <  5:222024/148‑1 (MQ=255)
    ccACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCggg                                                                                                                                                <  4:7243/148‑1 (MQ=255)
    ccACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCggg                                                                                                                                                <  8:321051/148‑1 (MQ=255)
    ccACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCgg                                                                                                                                                 <  6:175986/147‑1 (MQ=255)
    ccACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGgt                                                                                                                                               <  3:240295/149‑1 (MQ=255)
    ccACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGgt                                                                                                                                               <  4:264505/149‑1 (MQ=255)
    ccACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGgt                                                                                                                                               <  7:341618/149‑1 (MQ=255)
    ccACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGgt                                                                                                                                               <  2:111132/149‑1 (MQ=255)
     cacaAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgta                                                                                                                                              >  1:239217/1‑148 (MQ=255)
      acaAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCCACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtat                                                                                                                                             <  7:271230/149‑3 (MQ=255)
          gTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtattcgg                                                                                                                                         <  2:239217/149‑7 (MQ=255)
            ggCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCAGAATACCCGCAAGTGCGGGTGTACAAcc                                                                                                                                       >  2:114513/1‑149 (MQ=255)
             gcgcTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGACCTCACCCAGCGCAGGCTTTCCGACCTGCCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCAGAATACCCGCAAGTGCGGGTGTACAAccc                                                                                                                                      <  1:217889/149‑1 (MQ=255)
                   ttCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTa                                                                                                                                >  3:235149/1‑149 (MQ=255)
                        cGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAAc                                                                                                                           >  8:185272/1‑149 (MQ=255)
                             aGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGGCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAt                                                                                                                      <  4:73325/149‑1 (MQ=255)
                              gTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtg                                                                                                                     >  6:88329/1‑149 (MQ=255)
                              gTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtg                                                                                                                     >  4:127448/1‑149 (MQ=255)
                                     ggCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATAGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctgg                                                                                                                >  5:34460/1‑147 (MQ=255)
                                      gCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAg                                                                                                             >  8:48990/1‑149 (MQ=255)
                                        atcatcTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTc                                                                                                           >  8:110268/1‑149 (MQ=255)
                                           atcTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACATCCCTGATTACGAACGCAATGGCTGGCAGTCGAc                                                                                                        >  4:239826/1‑149 (MQ=255)
                                           atcTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGAc                                                                                                        <  4:235149/149‑1 (MQ=255)
                                                tctGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATa                                                                                                   >  3:344149/1‑149 (MQ=255)
                                                  tGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCAt                                                                                                    <  1:114513/146‑1 (MQ=255)
                                                   ggACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCg                                                                                                >  5:220813/1‑149 (MQ=255)
                                                    gACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGt                                                                                               <  3:255093/149‑1 (MQ=255)
                                                      cGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTgg                                                                                             <  7:48990/149‑1 (MQ=255)
                                                             aCCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGaa                                                                                        <  6:175441/147‑1 (MQ=255)
                                                             aCCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGaa                                                                                        <  6:34460/147‑1 (MQ=255)
                                                             aCCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGt                                                                                      >  8:91668/1‑149 (MQ=255)
                                                              cccAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGtg                                                                                     >  5:71421/1‑149 (MQ=255)
                                                               ccAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGtgc                                                                                    <  7:91668/149‑1 (MQ=255)
                                                                cAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGtgct                                                                                   <  3:127448/149‑1 (MQ=255)
                                                                      aGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAACTGCGGGTGTACAACCCTGATTACGAACGCAAAGGCTGGCAGTCGCCCCATACCGTGGTCGCAGTGCTGCACCa                                                                             >  2:125912/1‑149 (MQ=255)
                                                                         cTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGa                                                                          <  4:344149/149‑1 (MQ=255)
                                                                              cGACCTGGCCGGCTGCACCCTGTCAGGCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGGAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGc                                                                     <  6:41753/149‑1 (MQ=255)
                                                                              cGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACACATACCTTGGTCGAAGTGCTGCACCATGACCTGc                                                                     <  4:316308/149‑1 (MQ=255)
                                                                                   tGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTc                                                                >  8:328621/1‑149 (MQ=255)
                                                                                     gCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTc                                                                <  6:126193/147‑1 (MQ=255)
                                                                                     gCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTc                                                                <  6:71421/147‑1 (MQ=255)
                                                                                     gCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCt                                                              <  4:335630/149‑1 (MQ=255)
                                                                                     gCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCt                                                              >  8:167031/1‑149 (MQ=255)
                                                                                         gCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTc                                                          <  1:204766/149‑1 (MQ=255)
                                                                                              aCCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCa                                                                             >  7:185834/1‑125 (MQ=255)
                                                                                              aCCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCa                                                                             <  8:185834/125‑1 (MQ=255)
                                                                                                ccTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCgg                                                   >  7:228624/1‑149 (MQ=255)
                                                                                                 cTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGt                                                  <  7:185272/149‑1 (MQ=255)
                                                                                                          tGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCAcc                                           >  2:114586/1‑147 (MQ=255)
                                                                                                          tGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCAcc                                           >  5:262690/1‑147 (MQ=255)
                                                                                                            gcgcATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAg                                        <  3:249271/148‑1 (MQ=255)
                                                                                                              gcATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTc                                     <  7:167031/149‑1 (MQ=255)
                                                                                                                atcatcGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCaa                                   >  7:200034/1‑149 (MQ=255)
                                                                                                                      gAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAAccgccg                             >  6:270079/1‑149 (MQ=255)
                                                                                                                        gCGTTTCGACCCCGAATACCCGCAAGTGTGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTg                           >  5:176092/1‑149 (MQ=255)
                                                                                                                               gACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACa                     <  6:220813/148‑1 (MQ=255)
                                                                                                                               gACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAg                    >  4:119951/1‑149 (MQ=255)
                                                                                                                                 ccccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCCAGCTCAACCGCCGTGGGTACAGc                   >  7:49719/1‑148 (MQ=255)
                                                                                                                                   ccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATc                >  7:81889/1‑149 (MQ=255)
                                                                                                                                   ccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATc                <  7:328621/149‑1 (MQ=255)
                                                                                                                                    cGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGc                                                                     <  4:37356/95‑1 (MQ=255)
                                                                                                                                    cGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGc                                                                     >  3:37356/1‑95 (MQ=255)
                                                                                                                                    cGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATcc               >  5:240547/1‑149 (MQ=255)
                                                                                                                                      tacaCCCGCACTTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGc                   <  6:111146/140‑1 (MQ=255)
                                                                                                                                      tacaCCCGCACTTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGc                   >  5:111146/4‑143 (MQ=255)
                                                                                                                                         aCCCGCACTTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACAcc          >  3:283759/1‑149 (MQ=255)
                                                                                                                                           ccGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCt        >  5:13767/1‑149 (MQ=255)
                                                                                                                                            cGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTg       <  2:311664/149‑1 (MQ=255)
                                                                                                                                            cGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCAAGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCCCCGAGGCTACAGCAACCACAcccag       >  6:140873/1‑147 (MQ=255)
                                                                                                                                                 gTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAAac  <  3:239826/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAAAC  >  NC_002947/2367266‑2367559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑