Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,372,081 G→T 10.4% intergenic (+33/+79) gdhB → / ← PP_2081 NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,372,0810GT10.4% 131.6 / 3.5 47intergenic (+33/+79)gdhB/PP_2081NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein
Reads supporting (aligned to +/- strand):  ref base G (24/18);  new base T (2/3);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 6.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAA  >  NC_002947/2371942‑2372223
                                                                                                                                            |                                                                                                                                              
acgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCgg                                                                                                                                       >  3:310404/1‑149 (MQ=255)
  gacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCgggg                                                                                                                                     <  8:19163/149‑1 (MQ=255)
   cccTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCGGCATCACTGCCGGGGc                                                                                                                                    <  8:295871/148‑1 (MQ=255)
     cTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCtt                                                                                                                                  >  3:262701/1‑149 (MQ=255)
        cgcAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCATCACTGCCGGGGCttt                                                                                                                                 <  6:225350/147‑1 (MQ=255)
          cAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCttttttt                                                                                                                             >  2:289717/1‑149 (MQ=255)
             tGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTg                                                                                                                           >  5:178394/1‑148 (MQ=255)
                 aCGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGc                                                                                                                      >  7:150127/1‑149 (MQ=255)
                       aCTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggc                                                                                                                >  8:214118/1‑149 (MQ=255)
                            cTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggcgggc                                                                                                            >  3:155494/1‑148 (MQ=255)
                                  gATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcg                                                                                                     >  2:255763/1‑149 (MQ=255)
                                    tGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCGGCATCACTGCCgggg                                                                                                                                     >  8:67541/1‑115 (MQ=39)
                                    tGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAAGCCCCGGCATCACTGCCgggg                                                                                                                                     <  7:67541/115‑1 (MQ=39)
                                      tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCtttttttt                                                                                                                            >  6:144187/1‑122 (MQ=255)
                                      tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCGTGGGCGGGCCATTTcgctg                                                                                                  <  8:76160/148‑3 (MQ=255)
                                      tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCgg                                                                                                  <  3:292001/148‑1 (MQ=255)
                                      ctcgcgGTTGGCCACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCggg                                                                                                 >  5:281735/3‑149 (MQ=255)
                                        cgcgGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGt                                                                                                >  7:153716/1‑148 (MQ=255)
                                               gCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGc                                                                                        <  4:310404/149‑1 (MQ=255)
                                                 cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc                                                                                      >  5:197933/1‑149 (MQ=255)
                                                 cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc                                                                                      <  6:178394/149‑1 (MQ=255)
                                                     cgcgAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTa                                                                                   >  8:133535/1‑148 (MQ=255)
                                                      gcgAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACa                                                                                 <  5:93717/149‑1 (MQ=255)
                                                          gCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAgggg                                                                             >  8:294453/1‑149 (MQ=255)
                                                                cGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcgg                                                                       <  7:214118/149‑1 (MQ=255)
                                                                 gACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcggg                                                                      >  6:321614/1‑149 (MQ=255)
                                                                 gACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcggg                                                                      <  8:150127/149‑1 (MQ=255)
                                                                        ccATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGATCGTTTTAGTGTGGTAAATCAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTTTGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTa                                                               <  4:155494/149‑1 (MQ=39)
                                                                        ccATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGtt                                                                >  2:187534/1‑148 (MQ=255)
                                                                         cATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAc                                                              >  4:61162/1‑149 (MQ=255)
                                                                                  gcaggcagCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCg                                                      >  4:190203/1‑148 (MQ=255)
                                                                                    aggcagCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtct                                                   <  7:133535/149‑1 (MQ=255)
                                                                                     ggcagGGGTGGGGCCTTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGGGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCGGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctc                                                  <  6:28653/149‑1 (MQ=255)
                                                                                                tgCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCCCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCttt                                       >  7:18175/1‑149 (MQ=255)
                                                                                                       cgtaGCCTTGTAGTGTGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGAc                                 <  4:262701/145‑1 (MQ=255)
                                                                                                         gAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAg                              >  3:103256/1‑149 (MQ=255)
                                                                                                                   gTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGc                     <  8:153716/148‑1 (MQ=255)
                                                                                                                      atacaaaaaaaaGCCCCGGCATCACTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTGACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGa                  >  7:337172/5‑148 (MQ=38)
                                                                                                                        gTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctctc                                                >  7:304597/1‑116 (MQ=255)
                                                                                                                        gTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAAt               >  3:42012/1‑149 (MQ=255)
                                                                                                                           aaacaaacGCCCCGGCTGTCATGCGGGGGTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTCACCCCGCTCCACCAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAAtctc            <  3:286368/149‑1 (MQ=255)
                                                                                                                             aCAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCtt          <  1:187534/149‑1 (MQ=255)
                                                                                                                              cAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTc         <  7:294453/149‑1 (MQ=255)
                                                                                                                                   gCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc     <  8:18175/149‑1 (MQ=255)
                                                                                                                                   gCCCCGCCAGTGAGGCCGGGGCTTTTTTTTGTATGCCTCGGCGGGCCATTTCGCGGGTGAACCCGCGCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc     <  6:281735/149‑1 (MQ=255)
                                                                                                                                    ccccGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc     >  1:95171/1‑148 (MQ=255)
                                                                                                                                       cGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTGACTTGCACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCCaa  >  6:54222/1‑148 (MQ=255)
                                                                                                                                            |                                                                                                                                              
ACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAAGCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAA  >  NC_002947/2371942‑2372223

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: