| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,372,083 | G→A | 11.0% | intergenic (+35/+77) | gdhB → / ← PP_2081 | NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,372,083 | 0 | G | A | 11.0% | 121.7 / 2.9 | 47 | intergenic (+35/+77) | gdhB/PP_2081 | NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein |
| Reads supporting (aligned to +/- strand): ref base G (24/17); new base A (2/3); total (26/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
ACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCG > NC_002947/2371942‑2372230 | acgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCgg > 3:310404/1‑149 (MQ=255) gacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCgggg < 8:19163/149‑1 (MQ=255) cccTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAA‑GCCCCGGCATCACTGCCGGGGc < 8:295871/148‑1 (MQ=255) cTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCtt > 3:262701/1‑149 (MQ=255) cgcAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCATCACTGCCGGGGCttt < 6:225350/147‑1 (MQ=255) cAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCttttttt > 2:289717/1‑149 (MQ=255) tGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTg > 5:178394/1‑148 (MQ=255) aCGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGc > 7:150127/1‑149 (MQ=255) aCTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggc > 8:214118/1‑149 (MQ=255) cTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggcgggc > 3:155494/1‑148 (MQ=255) gATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcg > 2:255763/1‑149 (MQ=255) tGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAA‑GCCCCGGCATCACTGCCgggg > 8:67541/1‑115 (MQ=39) tGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAA‑GCCCCGGCATCACTGCCgggg < 7:67541/115‑1 (MQ=39) tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCtttttttt > 6:144187/1‑122 (MQ=255) tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCGTGGGCGGGCCATTTcgctg < 8:76160/148‑3 (MQ=255) tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCgg < 3:292001/148‑1 (MQ=255) ctcgcgGTTGGCCACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCggg > 5:281735/3‑149 (MQ=255) cgcgGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGt > 7:153716/1‑148 (MQ=255) gCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGc < 4:310404/149‑1 (MQ=255) cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc > 5:197933/1‑149 (MQ=255) cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc < 6:178394/149‑1 (MQ=255) cgcgAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTa > 8:133535/1‑148 (MQ=255) gcgAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACa < 5:93717/149‑1 (MQ=255) gCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAgggg > 8:294453/1‑149 (MQ=255) cGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcgg < 7:214118/149‑1 (MQ=255) gACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcggg < 8:150127/149‑1 (MQ=255) gACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcggg > 6:321614/1‑149 (MQ=255) ccATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGATCGTTTTAGTGTGGTAAATCAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTTTGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTa < 4:155494/149‑1 (MQ=39) ccATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGtt > 2:187534/1‑148 (MQ=255) cATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAc > 4:61162/1‑149 (MQ=255) gcaggcagCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCg > 4:190203/1‑148 (MQ=255) aggcagCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtct < 7:133535/149‑1 (MQ=255) ggcagGGGTGGGGCCTTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGGGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCGGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctc < 6:28653/149‑1 (MQ=255) tgCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCCCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCttt > 7:18175/1‑149 (MQ=255) cgtaGCCTTGTAGTGTGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGAc < 4:262701/145‑1 (MQ=255) gAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAg > 3:103256/1‑149 (MQ=255) gTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGc < 8:153716/148‑1 (MQ=255) atacaaaaaaaa‑GCCCCGGCATCACTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTGACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGa > 7:337172/5‑148 (MQ=38) gTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctctc > 7:304597/1‑116 (MQ=255) gTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAAt > 3:42012/1‑149 (MQ=255) aaacaaacGCCCCGGCTGTCATG‑CGGGGGTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTCACCCCGCTCCACCAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAAtctc < 3:286368/149‑1 (MQ=255) aCAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCtt < 1:187534/149‑1 (MQ=255) cAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTc < 7:294453/149‑1 (MQ=255) gCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc < 8:18175/149‑1 (MQ=255) gCCCCGCCAGTGAGGCCGGGGCTTTTTTTTGTATGCCTCGGCGGGCCATTTCGCGGGTGAACCCGCGCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc < 6:281735/149‑1 (MQ=255) ccccGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc > 1:95171/1‑148 (MQ=255) cGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTGACTTGCACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCCaa > 6:54222/1‑148 (MQ=255) gATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCg < 3:61162/148‑1 (MQ=255) | ACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCG > NC_002947/2371942‑2372230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |