Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,372,084 A→C 10.4% intergenic (+36/+76) gdhB → / ← PP_2081 NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,372,0840AC10.4% 122.0 / 4.9 48intergenic (+36/+76)gdhB/PP_2081NAD‑specific glutamate dehydrogenase/phosphoenolpyruvate synthase regulatory protein
Reads supporting (aligned to +/- strand):  ref base A (24/19);  new base C (2/3);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

ACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCGTG  >  NC_002947/2371942‑2372232
                                                                                                                                               |                                                                                                                                                    
acgacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCgg                                                                                                                                                >  3:310404/1‑149 (MQ=255)
  gacCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCgggg                                                                                                                                              <  8:19163/149‑1 (MQ=255)
   cccTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAA‑GCCCCGGCATCACTGCCGGGGc                                                                                                                                             <  8:295871/148‑1 (MQ=255)
     cTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCtt                                                                                                                                           >  3:262701/1‑149 (MQ=255)
        cgcAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCATCACTGCCGGGGCttt                                                                                                                                          <  6:225350/147‑1 (MQ=255)
          cAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCttttttt                                                                                                                                      >  2:289717/1‑149 (MQ=255)
             tGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTg                                                                                                                                    >  5:178394/1‑148 (MQ=255)
                 aCGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGc                                                                                                                               >  7:150127/1‑149 (MQ=255)
                       aCTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggc                                                                                                                         >  8:214118/1‑149 (MQ=255)
                            cTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTgggcgggc                                                                                                                     >  3:155494/1‑148 (MQ=255)
                                  gATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTcg                                                                                                              >  2:255763/1‑149 (MQ=255)
                                    tGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAA‑GCCCCGGCATCACTGCCgggg                                                                                                                                              >  8:67541/1‑115 (MQ=39)
                                    tGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAAAAAA‑GCCCCGGCATCACTGCCgggg                                                                                                                                              <  7:67541/115‑1 (MQ=39)
                                      tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCtttttttt                                                                                                                                     >  6:144187/1‑122 (MQ=255)
                                      tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCGTGGGCGGGCCATTTcgctg                                                                                                           <  8:76160/148‑3 (MQ=255)
                                      tACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCgg                                                                                                           <  3:292001/148‑1 (MQ=255)
                                      ctcgcgGTTGGCCACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCggg                                                                                                          >  5:281735/3‑149 (MQ=255)
                                        cgcgGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGt                                                                                                         >  7:153716/1‑148 (MQ=255)
                                               gCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGc                                                                                                 <  4:310404/149‑1 (MQ=255)
                                                 cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc                                                                                               >  5:197933/1‑149 (MQ=255)
                                                 cAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTc                                                                                               <  6:178394/149‑1 (MQ=255)
                                                     cgcgAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTa                                                                                            >  8:133535/1‑148 (MQ=255)
                                                      gcgAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACa                                                                                          <  5:93717/149‑1 (MQ=255)
                                                          gCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAgggg                                                                                      >  8:294453/1‑149 (MQ=255)
                                                                cGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcgg                                                                                <  7:214118/149‑1 (MQ=255)
                                                                 gACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcggg                                                                               <  8:150127/149‑1 (MQ=255)
                                                                 gACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGggggcggg                                                                               >  6:321614/1‑149 (MQ=255)
                                                                        ccATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGATCGTTTTAGTGTGGTAAATCAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTTTGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTa                                                                        <  4:155494/149‑1 (MQ=39)
                                                                        ccATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGtt                                                                         >  2:187534/1‑148 (MQ=255)
                                                                         cATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTAc                                                                       >  4:61162/1‑149 (MQ=255)
                                                                                  gcaggcagCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCg                                                               >  4:190203/1‑148 (MQ=255)
                                                                                    aggcagCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtct                                                            <  7:133535/149‑1 (MQ=255)
                                                                                     ggcagGGGTGGGGCCTTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGGGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCGGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctc                                                           <  6:28653/149‑1 (MQ=255)
                                                                                                tgCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCCCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCttt                                                >  7:18175/1‑149 (MQ=255)
                                                                                                       cgtaGCCTTGTAGTGTGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGAc                                          <  4:262701/145‑1 (MQ=255)
                                                                                                         gAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAg                                       >  3:103256/1‑149 (MQ=255)
                                                                                                                   gTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGc                              <  8:153716/148‑1 (MQ=255)
                                                                                                                      atacaaaaaaaa‑GCCCCGGCATCACTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTGACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGa                           >  7:337172/5‑148 (MQ=38)
                                                                                                                        gTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGtctctc                                                         >  7:304597/1‑116 (MQ=255)
                                                                                                                        gTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAAt                        >  3:42012/1‑149 (MQ=255)
                                                                                                                           aaacaaacGCCCCGGCTGTCATG‑CGGGGGTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTCACCCCGCTCCACCAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAAtctc                     <  3:286368/149‑1 (MQ=255)
                                                                                                                             aCAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCtt                   <  1:187534/149‑1 (MQ=255)
                                                                                                                              cAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTc                  <  7:294453/149‑1 (MQ=255)
                                                                                                                                   gCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc              <  8:18175/149‑1 (MQ=255)
                                                                                                                                   gCCCCGCCAGTGAGGCCGGGGCTTTTTTTTGTATGCCTCGGCGGGCCATTTCGCGGGTGAACCCGCGCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc              <  6:281735/149‑1 (MQ=255)
                                                                                                                                    ccccGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCAcc              >  1:95171/1‑148 (MQ=255)
                                                                                                                                       cGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTGACTTGCACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCCaa           >  6:54222/1‑148 (MQ=255)
                                                                                                                                              gATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCg    <  3:61162/148‑1 (MQ=255)
                                                                                                                                               aTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACtt                                                                     <  8:15489/82‑1 (MQ=255)
                                                                                                                                               aTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACtt                                                                     >  7:15489/1‑82 (MQ=255)
                                                                                                                                               aTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCGTg  <  4:103256/149‑1 (MQ=255)
                                                                                                                                               aTGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCGTg  <  5:321614/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
ACGACCTGCGCAATGCCACGGGCACTGACTATGCGATGTACGCGGTGGCCAACCGCGAGCTGGTCGACCTGGCCATGAGCGGGCAGGCAGCGGTGGTGCCGTCCTGAGCCTTGTAGTGAGGTAAAACAAA‑GCCCCGGCAGTGATGCCGGGGCTTTTTTTTGTATGCCTGGGCGGGCCATTTCGCGGGTGAACCCGCTCCTACAGGGGGGGCGGGGCTGTTACTTGAACCGTCTCTCCACGCCTTTTTCGACCAGGATCTTGGCGGAAATCTCTTCCACCGAAAAATGCGTG  >  NC_002947/2371942‑2372232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑