Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,405,232 G→T 10.9% V77L (GTG→TTG)  PP_2108 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,405,2320GT10.9% 154.9 / 8.2 55V77L (GTG→TTG) PP_2108hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/26);  new base T (2/4);  total (25/30)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

GAAGCCAGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAC  >  NC_002947/2405091‑2405374
                                                                                                                                             |                                                                                                                                              
gAAGCCAGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAg                                                                                                                                          <  7:132154/148‑1 (MQ=255)
    ccAGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCCCTTGCACGTGCTg                                                                                                                                     <  4:39837/149‑1 (MQ=255)
     cAGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCCCTTGCACGTGCTgg                                                                                                                                    <  5:189260/149‑1 (MQ=255)
      aGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTggc                                                                                                                                   >  4:258906/1‑149 (MQ=255)
      aGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTggc                                                                                                                                   <  1:317435/149‑1 (MQ=255)
      aGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGGCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTggc                                                                                                                                   <  2:190959/149‑1 (MQ=255)
        ggCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTggcgg                                                                                                                                 >  8:262491/1‑149 (MQ=255)
          ccAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGgcggcg                                                                                                                               >  8:220479/1‑149 (MQ=255)
            aaCCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGa                                                                                                                             >  2:64314/1‑149 (MQ=255)
             aCCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGAc                                                                                                                            <  5:3504/149‑1 (MQ=255)
              ccTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACt                                                                                                                           <  2:183170/149‑1 (MQ=255)
               cTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACtt                                                                                                                          >  6:303472/1‑149 (MQ=255)
                tGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCCCTTGCACGTGCt                                                                                                                                      >  6:355122/1‑136 (MQ=255)
                tGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCCCTTGCACGTGCt                                                                                                                                      <  5:355122/136‑1 (MQ=255)
                   aaTTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACg                                                                                                                      <  7:220479/149‑1 (MQ=255)
                   aaTTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACg                                                                                                                      <  4:320002/149‑1 (MQ=255)
                    aTTACTGTGTGGTGCGCTGGAACAGGTAGGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACgg                                                                                                                     >  1:208314/1‑149 (MQ=255)
                           tgtgGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACg                                                                                                                      >  3:227330/1‑141 (MQ=255)
                           tgtgGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACg                                                                                                                      <  4:227330/141‑1 (MQ=255)
                             tggtgCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAg                                                                                                             <  4:184057/148‑1 (MQ=255)
                                  cgcctcaaCAGGTACGTGCGTTGCTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGGCAATCGGTTGATCTGTCGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGc                                                                                                       <  8:140087/143‑1 (MQ=255)
                                   gcTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGTAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCCCTTGCACGTGCTggctatc                                                                                                                               <  1:112594/124‑5 (MQ=255)
                                   gcTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCCCTTGCACGTGCTggctatc                                                                                                                               >  2:112594/1‑120 (MQ=255)
                                       gAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCcgcg                                                                                                  >  4:187730/1‑149 (MQ=255)
                                          cAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCga                                                                                               <  3:187730/149‑1 (MQ=255)
                                              tACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGAcc                                                                                           <  8:98220/149‑1 (MQ=255)
                                                 gTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTc                                                                                        >  3:342185/1‑149 (MQ=255)
                                                     gttgttTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGc                                                                                    >  8:223749/1‑149 (MQ=255)
                                                      ttgttTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATc                                                                                                                >  4:153992/1‑120 (MQ=255)
                                                      ttgttTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATc                                                                                                                <  3:153992/120‑1 (MQ=255)
                                                            gACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAAt                                                                                <  1:65245/146‑1 (MQ=255)
                                                            gACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAAt                                                                                >  2:65245/1‑146 (MQ=255)
                                                                 gcCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCgaga                                                                                             <  8:272516/128‑1 (MQ=255)
                                                                 gcCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCgaga                                                                                             >  7:272516/1‑128 (MQ=255)
                                                                    catcatcatCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTg                                                                     <  8:112907/149‑1 (MQ=255)
                                                                           atcaGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGcc                                                              <  2:208314/149‑1 (MQ=255)
                                                                            tcaGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCACCAAGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCAGGCGAATCAGCAAAAGTGCGGTGccc                                                             >  6:379677/1‑149 (MQ=255)
                                                                               gcagcGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTc                                                          >  5:76136/1‑149 (MQ=255)
                                                                                   cGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGATGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATc                                                      <  7:262491/149‑1 (MQ=255)
                                                                                      gTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGc                                                   <  1:64314/149‑1 (MQ=255)
                                                                                             aGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACg                                                                                                                      >  8:265054/1‑75 (MQ=255)
                                                                                             aGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACg                                                                                                                      <  7:265054/75‑1 (MQ=255)
                                                                                                 aaaGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGaa                                        <  5:303472/149‑1 (MQ=255)
                                                                                                 aaaGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGaa                                        >  6:61652/1‑149 (MQ=255)
                                                                                                      gTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGa                                   <  3:258906/149‑1 (MQ=255)
                                                                                                         aaTCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACa                                 <  4:337717/148‑1 (MQ=255)
                                                                                                          aTCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGa                                   >  3:2109/1‑145 (MQ=255)
                                                                                                           tCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGAt                              >  2:124238/1‑149 (MQ=255)
                                                                                                              gTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATggg                           >  6:54967/1‑149 (MQ=255)
                                                                                                               ttGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGt                          <  7:223749/149‑1 (MQ=255)
                                                                                                                    cGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCAt                      <  4:2109/148‑1 (MQ=255)
                                                                                                                         gcgcCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGCACCCATCTGTc                 <  4:342185/148‑1 (MQ=255)
                                                                                                                           gcCTCCGATAGCCAGCACGTGCAAGGGCTGNCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGt              >  1:34153/1‑149 (MQ=255)
                                                                                                                                cGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGAcgc         >  2:196551/1‑149 (MQ=255)
                                                                                                                                       cAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAc  >  4:347684/1‑149 (MQ=255)
                                                                                                                                             |                                                                                                                                              
GAAGCCAGGGCCAACCTGCAATTACTGTGTGGTGCGCTGGAACAGGTACGTGCGTTGTTTGACGCGCCCATCATCATCAGCAGCGGGTATCGCAGCGAAAGGGTCAATCGGTTGATCGGTGGCGCCTCCGATAGCCAGCACGTGCAAGGGCTGGCGGCGGACTTCACGGTGATCGAGGTCAGCCCGCGCGAGACCGTCCGGCGAATCAGCAAAAGTGCGGTGCCCTTCGATCAGCTGATCCTGGAATTCGACAGATGGGTGCATCTGTCGGTGACGCGCGGCAC  >  NC_002947/2405091‑2405374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑