Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,440,530 T→A 8.5% V7E (GTA→GAA)  PP_2138 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,440,5300TA 8.5% 131.6 / 3.1 47V7E (GTA→GAA) PP_2138hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/19);  new base A (2/2);  total (26/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

GGCCTGGGCCAAGGTATCACCACTGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTGGGTGCGTCCCCTTTCGATGTTCCAG  >  NC_002947/2440394‑2440666
                                                                                                                                         |                                                                                                                                        
ggCCTGGGCCAAGGTATCACCACTGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCAtt                                                                                                                               >  4:2984/1‑148 (MQ=255)
   cTGGGCCAAGGTATCACCACTGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGGATTACa                                                                                                                            >  8:305308/1‑148 (MQ=255)
          aaGGTATCACCACTGTCTTCGAACGCGGCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCt                                                                                                                    <  6:285706/149‑1 (MQ=255)
            ggTATCACCACTGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGa                                                                                                                  <  3:2984/149‑1 (MQ=255)
             gTATCACCACTGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCt                                                                                                                    >  8:104194/1‑146 (MQ=255)
             gTATCACCACTGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGa                                                                                                                  <  7:104194/148‑1 (MQ=255)
                 caccacTGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTAcc                                                                                                             >  3:10717/1‑149 (MQ=255)
                   ccacTGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCgt                                                                                                           <  2:226564/149‑1 (MQ=255)
                    cacTGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGAATACCGGCATTACAAAGGGCCTGAGTACCgtg                                                                                                          >  4:35405/1‑149 (MQ=255)
                       tGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTa                                                                                                               <  1:98753/141‑1 (MQ=255)
                       tGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTa                                                                                                               >  2:98753/1‑141 (MQ=255)
                       tGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCt                                                                                                       >  6:366186/1‑149 (MQ=255)
                                 cgcgTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTgc                                                                                             <  3:339611/149‑1 (MQ=255)
                                              gTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGa                                                                                 >  7:356121/1‑148 (MQ=255)
                                                 gCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTGTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAAcc                                                                             <  1:80470/149‑1 (MQ=255)
                                                  cGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTAAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAAcc                                                                             >  8:176787/1‑148 (MQ=255)
                                                    ggACAGGAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTATCATGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACATGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGaa                                                                          <  8:356121/149‑1 (MQ=255)
                                                    ggACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCg                                                                            >  8:47430/1‑147 (MQ=255)
                                                    ggACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCg                                                                            >  8:125333/1‑147 (MQ=255)
                                                        agcagcCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGAATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGt                                                                      >  1:196230/1‑149 (MQ=255)
                                                         gcagcCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTg                                                                     <  4:10717/149‑1 (MQ=255)
                                                          cagcCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTg                                                                     >  6:157551/1‑148 (MQ=255)
                                                           aaccGGGGCACTAG‑GCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGAACTCCGAAACCGAAGAGTGGa                                                                   >  3:230155/3‑149 (MQ=255)
                                                                gggC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTAAAAAGGGCCTGAGTACCGTGTCTTAAGTGTTGCGCGGCACTCCGAAACCGAAGAGTgagatcg                                                               >  7:310302/1‑142 (MQ=255)
                                                                gggC‑CCAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGt                                                                      <  8:310302/141‑1 (MQ=255)
                                                                      tAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCt                                                          >  2:361440/1‑148 (MQ=255)
                                                                      tAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACAGGCATTACAAAGGGCCTGAGTACCGTGTCTTAAGTGTTGAGAGGAACTCCGAAACCGAAGAGTGTATGGTGTTCt                                                          >  7:24792/1‑148 (MQ=255)
                                                                             cctgttttgttttTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGAATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGc                                                  <  3:35405/146‑1 (MQ=255)
                                                                              cGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGcc                                                 >  2:164483/1‑149 (MQ=255)
                                                                                     gttttTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATgg                                          >  7:115830/1‑149 (MQ=255)
                                                                                             aGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTAc                                   <  8:115830/148‑1 (MQ=255)
                                                                                              ggTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTgg                                                                    >  6:291783/1‑114 (MQ=255)
                                                                                              ggTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTgg                                                                    <  5:291783/114‑1 (MQ=255)
                                                                                               gTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGc                                >  1:227982/1‑149 (MQ=255)
                                                                                                   ttttCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTc                             <  1:164483/148‑1 (MQ=255)
                                                                                                      tCAGGAGGTGTGCAACATGCAGATACAACCAGGCGAATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTgg                          <  2:196230/148‑1 (MQ=255)
                                                                                                       cAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGt                                         >  2:250599/1‑132 (MQ=255)
                                                                                                       cAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGt                                         <  1:250599/132‑1 (MQ=255)
                                                                                                         ggaggTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTAGCACTGCGTGTAGGGTGATTACCGCTTCTGGGTGc                      >  8:297581/1‑149 (MQ=255)
                                                                                                           aggTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTGGGTGCGt                    <  7:176787/149‑1 (MQ=255)
                                                                                                                tgCAACATGCAGATACAACCAGGCGTATACCGGCATTACAACGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTGGGTGCGTCCCCt               <  2:227982/149‑1 (MQ=255)
                                                                                                                  cAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTGGGTGCGTCCCCttt             >  4:350067/1‑149 (MQ=255)
                                                                                                                    aCATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTGGGTGCGTCCCCTTTc            >  5:60601/1‑148 (MQ=255)
                                                                                                                     cATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTGGGTGCGTCCCCTTTCGa          <  4:179846/149‑1 (MQ=255)
                                                                                                                             tACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTGGGTGCGTCCCCTTTCGATGTTCCa   <  7:125333/148‑1 (MQ=255)
                                                                                                                             tACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTGGGTGCGTCCCCTTTCGATGTTCCAg  <  7:47430/149‑1 (MQ=255)
                                                                                                                             tACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTGGGTGCGTCCCCTTTCGATGTTCCAg  >  3:45774/1‑149 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GGCCTGGGCCAAGGTATCACCACTGTCTTCGAACGCGTCTGATCGCGTAGCGGGACAGCAGCCGGGGC‑CTAGTGCCCCGGTTTTGTTTTTTCAGGTTTTTTTCAGGAGGTGTGCAACATGCAGATACAACCAGGCGTATACCGGCATTACAAAGGGCCTGAGTACCGTGTCTTCAGTGTTGCGCGGCACTCCGAAACCGAAGAGTGGATGGTGTTCTACCAATGCCTGTATGGTGATTACAGCTTCTGGGTGCGTCCCCTTTCGATGTTCCAG  >  NC_002947/2440394‑2440666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑