Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,444,424 G→T 6.6% R156S (CGC→AGC)  sulA ← cell division inhibitor SulA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,444,4240GT 6.6% 194.1 / 3.8 61R156S (CGC→AGC) sulAcell division inhibitor SulA
Reads supporting (aligned to +/- strand):  ref base G (33/24);  new base T (2/2);  total (35/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.36e-01

CCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTT  >  NC_002947/2444277‑2444555
                                                                                                                                                   |                                                                                                                                   
ccATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCg                                                                                                                                     >  5:319647/1‑148 (MQ=255)
  aTGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGAt                                                                                                                                  <  4:229931/149‑1 (MQ=255)
    gCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGt                                                                                                                                >  1:60259/1‑149 (MQ=255)
       cAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCCGCCTCGGCTGATGTTCa                                                                                                                             <  7:87921/149‑1 (MQ=255)
        aGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTc                                                                                                                              <  2:210627/147‑1 (MQ=255)
        aGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAg                                                                                                                            <  2:269194/149‑1 (MQ=255)
        aGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGACAAAGCCTGAACATCCGCCTCGGCTGATGTTCAg                                                                                                                            <  3:354939/149‑1 (MQ=39)
          tgtgGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGc                                                                                                                          <  3:69110/149‑1 (MQ=255)
              ggCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTtg                                                                                                                      >  3:355830/1‑149 (MQ=255)
               gCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTtgt                                                                                                                     <  8:10734/149‑1 (MQ=255)
               gCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTtgt                                                                                                                     <  4:341311/149‑1 (MQ=255)
                cGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTtgtg                                                                                                                    <  6:192770/149‑1 (MQ=255)
                    cAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCAGAGGCGTATGTTCAGGCTTTGTGCAtt                                                                                                                >  6:268329/1‑149 (MQ=255)
                        tGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCg                                                                                                            >  7:77665/1‑149 (MQ=255)
                          gCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCgg                                                                                                           <  3:240476/148‑1 (MQ=255)
                          gCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCgg                                                                                                           >  4:321045/1‑148 (MQ=255)
                              gTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTg                                                                                                      >  3:56404/1‑149 (MQ=255)
                                     gatggatgGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCAc                                                                                               >  4:158098/1‑149 (MQ=255)
                                         gatggCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAg                                                                                           <  6:319647/149‑1 (MQ=255)
                                              cGAGCTCGATCGTTATGAAGGGCCGCTTGTGCTGCACTGAGTGTTCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAgtt                                                                                      <  4:280823/149‑1 (MQ=255)
                                              cGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTcc                                                                                                             >  8:90644/1‑126 (MQ=255)
                                              cGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTcc                                                                                                             <  7:90644/126‑1 (MQ=255)
                                              cGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAgtt                                                                                      <  2:60259/149‑1 (MQ=255)
                                                     gAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGc                                                                                <  3:321045/148‑1 (MQ=255)
                                                              gaAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCgg                                                                       >  4:241184/1‑148 (MQ=255)
                                                               aaGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCa                                                                     >  8:289205/1‑149 (MQ=255)
                                                                aGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCAt                                                                    >  3:177705/1‑149 (MQ=255)
                                                                 gggCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTAGCAGGCTATTTGAAGTGACAGGCAAAGCCTGATCATCAGGCGAGGCGGATGTTCAGGCTTTGTGCAGTTCCGGCTGTGGGTGCCGGGAGCTGTTGTTGGCGGGGAGGGGctgt                                                                   >  4:295842/1‑146 (MQ=255)
                                                                      gcgTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGt                                                              >  3:47322/1‑149 (MQ=255)
                                                                          gtgCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGcc                                                          >  2:135896/1‑149 (MQ=255)
                                                                             ctgcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCcca                                                        <  4:355830/148‑1 (MQ=255)
                                                                              tgcACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCccagc                                                      >  2:54152/1‑149 (MQ=255)
                                                                                   tGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCGGCCAGCt                                                 >  8:318690/1‑149 (MQ=255)
                                                                                   tGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCt                                                 >  4:347448/1‑149 (MQ=255)
                                                                                      gtgtGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTcac                                              >  5:73750/1‑149 (MQ=255)
                                                                                      gtgtGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTcac                                              <  4:56404/149‑1 (MQ=255)
                                                                                        gtgCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTcacca                                            >  4:194447/1‑149 (MQ=255)
                                                                                           cagcagCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCACTTCCGGCACTTGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACgg                                         <  5:268329/149‑1 (MQ=255)
                                                                                            agcagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgt                                        >  4:59180/1‑149 (MQ=255)
                                                                                            agcagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgt                                        <  1:135896/149‑1 (MQ=255)
                                                                                              cagcGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGgtgt                                      <  4:177705/149‑1 (MQ=255)
                                                                                                    aTCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCt                                  >  2:297673/1‑147 (MQ=255)
                                                                                                    aTCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCt                                  >  1:332184/1‑147 (MQ=255)
                                                                                                     tCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAg                               >  6:141912/1‑149 (MQ=255)
                                                                                                        ggCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGcc                            >  6:171651/1‑149 (MQ=255)
                                                                                                         gCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGcc                            >  5:6706/1‑148 (MQ=255)
                                                                                                            aTTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAgg                        >  2:22201/1‑149 (MQ=255)
                                                                                                              ttGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTg                      >  5:46208/1‑149 (MQ=255)
                                                                                                                  aGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCaaa                  <  3:158098/149‑1 (MQ=255)
                                                                                                                   gTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAg                         >  6:153731/1‑141 (MQ=255)
                                                                                                                   gTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAg                         <  5:153731/141‑1 (MQ=255)
                                                                                                                    tGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGc                <  2:332184/149‑1 (MQ=255)
                                                                                                                    tGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGc                <  3:194447/149‑1 (MQ=255)
                                                                                                                        aGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTca            <  1:54152/149‑1 (MQ=255)
                                                                                                                          gCAAAGCCTGAACATCAGCCGAGGCGGATGTTCTGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTca            >  2:30155/1‑147 (MQ=255)
                                                                                                                           cAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAg         >  1:239058/1‑149 (MQ=255)
                                                                                                                             aaGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGc       >  4:91484/1‑149 (MQ=255)
                                                                                                                                 cTGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGt   >  5:60314/1‑149 (MQ=255)
                                                                                                                                  tGAACATCCGCCTCGGCTGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGtt  >  4:275406/1‑149 (MQ=255)
                                                                                                                                  tGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGtt  >  6:65919/1‑149 (MQ=255)
                                                                                                                                         cAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGt                       >  7:133316/1‑121 (MQ=255)
                                                                                                                                         cAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGt                       <  8:133316/121‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                   
CCATGCTCAGTGTGGGCGTGCAGATGGCCGGTCGCCTGATGGATGGCGAGCTCGAGCGTGATGAAGGGCCGCGTGTGCTGCACTGAGTGTGCAGCAGCGTATCAGGCTATTTGAAGTGACAGGCAAAGCCTGAACATCAGCCGAGGCGGATGTTCAGGCTTTGTGCATTTCCGGCACTGGCTGCACGTAGCAGTTGTTGGCGTGCAGCGGCATTGACGTTGCCCAGCCAGCTCACCACGGTGTGGCTGAGGCCCAGGTGCAAAGCCTCACAGGCCAGTT  >  NC_002947/2444277‑2444555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑