Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,455,264 A→C 7.6% intergenic (‑146/‑22) mfd ← / → gapB transcription‑repair coupling factor/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,455,2640AC 7.6% 157.0 / 2.3 52intergenic (‑146/‑22)mfd/gapBtranscription‑repair coupling factor/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (22/26);  new base C (2/2);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

TGCACTCCAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGCCCTGGCTGAAGCGATGATCCCGCTTATCGGTCAGCTCTACCGCAATAACAATGTGGTGAG  >  NC_002947/2455126‑2455410
                                                                                                                                          |                                                                                                                                                  
tGCACTCCAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCttttt                                                                                                                                          >  2:332763/1‑149 (MQ=255)
 gCACTCCAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCTACATTATGACGccc                                                                                                                                                >  1:219044/1‑142 (MQ=255)
 gCACTCCAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCTACATTATGACGccc                                                                                                                                                <  2:219044/142‑1 (MQ=255)
  cACTCCAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCtttttt                                                                                                                                         <  1:332763/148‑1 (MQ=255)
  cACTCCAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTc                                                                                                                                        <  1:180500/149‑1 (MQ=255)
     tCCAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCTACATTATGACGccccaac                                                                                                                                            <  4:197569/142‑4 (MQ=255)
     tCCAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCTACATTATGACGccccaac                                                                                                                                            >  3:197569/1‑139 (MQ=255)
     tCCAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGt                                                                                                                                     >  4:241377/1‑149 (MQ=255)
      gcAAGCGTTGCGGCATAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATTAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCt                                                                                                                                       <  6:117404/145‑1 (MQ=255)
      ccAAGAGATGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGAGTCATAATGTAGCCCCTTTATTCt                                                                                                                                       >  5:117404/1‑146 (MQ=255)
       cAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGt                                                                                                                                     <  6:314397/147‑1 (MQ=255)
        aaGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTccc                                                                                                                                  >  5:232708/1‑149 (MQ=255)
        aaGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTccc                                                                                                                                  <  6:63198/149‑1 (MQ=255)
            gTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACa                                                                                                                              <  3:371500/149‑1 (MQ=255)
               gCGGCAACGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGACAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGGTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACAtgt                                                                                                                           <  2:222040/149‑1 (MQ=255)
                    aaaGTGACCGATTGTACCGGTGACAGTGCCGGCTATCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAg                                                                                                                      >  1:99096/1‑149 (MQ=255)
                      agagACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGt                                                                                                                    >  3:229404/1‑149 (MQ=255)
                            cGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTAACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTTTCCCTACATGTGGAAGGTTCACGt                                                                                                              >  7:266685/1‑149 (MQ=255)
                                  tACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCa                                                                                                        <  1:301349/149‑1 (MQ=255)
                                    ccGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAg                                                                                                       >  3:203399/1‑148 (MQ=255)
                                              tGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGAc                                                                                              >  6:336899/1‑147 (MQ=255)
                                              tGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCa                                                                                            <  3:241377/149‑1 (MQ=255)
                                                       tCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCtt                                                                                     <  5:189131/147‑1 (MQ=255)
                                                         aGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGcc                                                                                                  <  6:356876/132‑1 (MQ=255)
                                                         aGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGcc                                                                                                  >  5:356876/1‑132 (MQ=255)
                                                           gCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAg                                                                               >  6:308565/1‑149 (MQ=255)
                                                            cTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGt                                                                              >  3:196384/1‑149 (MQ=255)
                                                             tCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTg                                                                             >  7:252644/1‑149 (MQ=255)
                                                                aaaGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGAt                                                                          <  4:196384/149‑1 (MQ=255)
                                                                    ggCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGAt                                                                      <  6:232708/149‑1 (MQ=255)
                                                                     gCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATc                                                                     <  4:170275/149‑1 (MQ=255)
                                                                     gCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATc                                                                     >  3:170275/1‑149 (MQ=255)
                                                                        tGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTg                                                                  <  2:99096/149‑1 (MQ=255)
                                                                          aaCACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGaa                                                                <  5:336899/149‑1 (MQ=255)
                                                                            cacTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTc                                                                                                                  >  6:276728/1‑97 (MQ=255)
                                                                            cacTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTc                                                                                                                  <  5:276728/97‑1 (MQ=255)
                                                                             acTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGcc                                                             >  4:345830/1‑149 (MQ=255)
                                                                                      cGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGCCCTGGCTGaa                                                    <  1:215293/149‑1 (MQ=255)
                                                                                      cGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGCCCTGGCTGaa                                                    <  5:308565/149‑1 (MQ=255)
                                                                                               gACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGCCCTGGCTGAAGCGATGATc                                           <  4:229404/149‑1 (MQ=255)
                                                                                                    gCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGccc                                                            <  8:162540/127‑1 (MQ=255)
                                                                                                       cTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGCCCTGGCTGAAGCGATGATCCCGCTTAt                                   <  3:345830/149‑1 (MQ=255)
                                                                                                        tCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCg                                                                    <  5:319969/115‑1 (MQ=255)
                                                                                                        tCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCg                                                                    >  6:319969/1‑115 (MQ=255)
                                                                                                          aTTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGCCCTGGCTGAAGCGATGATCCCGCTTATCgg                                >  6:61625/1‑149 (MQ=255)
                                                                                                             ggcGGTTCCGCGTTGGTGCTTCATAATGTAGCGCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCAGGACCAGTGTGTTGGTGAGTGGATTTATCGTGAAGCCCTGGCTGAAGCGATGATCCCGCTTATCGGTc                              <  8:266685/146‑1 (MQ=255)
                                                                                                                      gcgTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGCCCTGGCTGAAGCGATGATCCCGCTTATCGGTCAGCTCTACCg                    >  5:169224/1‑149 (MQ=255)
                                                                                                                        gTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGccc                                                                                                 >  4:113044/1‑70 (MQ=255)
                                                                                                                        gTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGccc                                                                                                 <  3:113044/70‑1 (MQ=255)
                                                                                                                                   aTAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGCCCTGGCTGAAGCGATGATCCCGCTTATCGGTCAGCTCTACCGCAATAACAAtgtg       <  8:252644/149‑1 (MQ=255)
                                                                                                                                   aTAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCAGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGCCCTGGCTGAAGCGATGATCCCGCTTATCGGTCAGCTCTACCGCAATAACAAtgtg       >  1:72176/1‑149 (MQ=255)
                                                                                                                                        gTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATAGATCGTGAAGCCCTGGCTGAAGCGATGATCCCTCTTATCTGTAAGCTCTACCGCAATAACAATGTGGTGAg  >  3:205624/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                                  
TGCACTCCAAGCGTTGCGGCAAAGAGACCGATTGTACCGGTGACAGTGCCGGCTGTCAGGCTCGAAAGGGCATGAACACTGGCTTGCGTCACTGCGACAGGCACTCATTGCCGGTTCCGCGTTGGGGCGTCATAATGTAGCCCCTTTTTTCTGTCCCTACATGTGGAAGGTTCCCGTGACTCAGAAGCCCGACCAGTGTCTTGGTGAGTGGATTGATCGTGAAGCCCTGGCTGAAGCGATGATCCCGCTTATCGGTCAGCTCTACCGCAATAACAATGTGGTGAG  >  NC_002947/2455126‑2455410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑