| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,467,909 | A→G | 7.8% | S267G (AGC→GGC) | queF → | NADPH‑dependent 7‑cyano‑7‑deazaguanine reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,467,909 | 0 | A | G | 7.8% | 143.9 / 3.0 | 51 | S267G (AGC→GGC) | queF | NADPH‑dependent 7‑cyano‑7‑deazaguanine reductase |
| Reads supporting (aligned to +/- strand): ref base A (25/22); new base G (2/2); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.81e-01 | |||||||||||
CCAGCATGCCGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCGAAGCGCT > NC_002947/2467761‑2468052 | ccAGCATGCCGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCa < 1:292541/149‑1 (MQ=255) tGCCGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCg > 6:334887/1‑148 (MQ=255) cGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑TGATCGGCCCggtgc < 7:15561/149‑4 (MQ=255) gACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAAGCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTAGGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATaa < 3:95726/149‑1 (MQ=255) cATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACg > 2:293497/1‑149 (MQ=255) aGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCt < 5:234405/148‑1 (MQ=255) gtgtgtGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCgg > 3:17559/1‑131 (MQ=255) gtgtgtGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCgg < 4:17559/131‑1 (MQ=255) gtgGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑AGATCAGCCCGGATAACAAACGGCTGGTCCGGCa > 7:224595/1‑149 (MQ=255) aGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTaa > 3:168891/1‑149 (MQ=255) gCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCACCCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTaa > 6:168823/1‑148 (MQ=255) tACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAggg < 8:42489/148‑1 (MQ=255) aacTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAgggg < 4:214003/146‑1 (MQ=255) ttGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCAATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCACCCCGGATAACAAACGGCTGGTCCGGAAGTAATACAGAGCGCCCCATTg > 4:280253/1‑149 (MQ=255) aagaaTCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCg < 3:280253/148‑1 (MQ=255) tCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGc > 1:119479/1‑110 (MQ=255) tCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGc < 2:119479/110‑1 (MQ=255) tgctgcAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGc > 7:147431/1‑149 (MQ=255) ctgcAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCAAAGCGGccccc < 5:334887/145‑1 (MQ=255) tgcAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTAt > 8:130272/1‑149 (MQ=255) aGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCTCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTc < 1:41387/149‑1 (MQ=255) aGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCAAAGCGGccccc < 4:326373/141‑1 (MQ=255) aGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCAAAGCGGccccc > 3:326373/1‑141 (MQ=255) gAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATc > 4:116532/1‑149 (MQ=255) ccTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACgg < 3:160898/91‑1 (MQ=255) ccTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACgg > 4:160898/1‑91 (MQ=255) tGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGc > 1:228071/1‑149 (MQ=255) gTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCtgtg > 8:68900/1‑148 (MQ=255) tCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCtgtg > 3:150745/1‑147 (MQ=255) tCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCtgtg > 2:310016/1‑147 (MQ=255) aTGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑Gc > 3:289216/1‑148 (MQ=255) tGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGCTCGTTCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATCCAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCg > 1:102735/1‑149 (MQ=255) tGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCg < 3:116532/148‑1 (MQ=255) cgcgcTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTAttt > 2:170924/1‑124 (MQ=255) cgcgcTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTAttt < 1:170924/124‑1 (MQ=255) cgcTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTg < 7:130272/149‑1 (MQ=255) gcgGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCa < 8:224595/149‑1 (MQ=255) gTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAggg < 2:228071/149‑1 (MQ=255) ggCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGGGTgg < 8:147431/149‑1 (MQ=255) ggCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCACCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGTGTgg > 2:365363/1‑149 (MQ=255) ggCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGGCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGGGTgg < 1:130993/149‑1 (MQ=255) ggACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCa > 5:358902/1‑118 (MQ=255) ggACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCa < 6:358902/118‑1 (MQ=255) aCATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGGGTGGCAGt > 3:78689/1‑147 (MQ=255) cAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGGGt > 5:63220/1‑137 (MQ=255) aaCCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCg > 7:155944/1‑149 (MQ=255) aaCCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCg < 8:204135/149‑1 (MQ=255) cagcaCCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGc < 1:310016/149‑1 (MQ=255) tccGGGC‑TGATCGGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTc > 1:189253/2‑148 (MQ=255) ccGGGC‑TGATCGGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCa < 1:78301/116‑1 (MQ=255) ccGGGC‑TGATCGGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCa > 2:78301/1‑116 (MQ=255) gATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCATCCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCGAAGCGCt < 1:293497/149‑1 (MQ=255) | CCAGCATGCCGACTTCCATGAGCAGTGTGTGGAGCGGATTTACCTGGACTTGAAGAATCTGCTGCAGCCGGAGCACCTGACGGTCTATGCGCGCTATGTGCGCCGCGGTGGGCTGGACATCAACCCGTATCGCAGCACCGGGC‑CGATCAGCCCGGATAACAAACGGCTGGTCCGGCAGTAATACAGGGGGCCGCTTTGCGGCCCCCGCTATTTCAGATCCCCATGCTGTGCA‑GCGAGTTGGCAATGCTGCGCAGGGTGGCAGTGAGGTCGGGGTGGTCGGCCTCGAAGCGCT > NC_002947/2467761‑2468052 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |