Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,474,148 T→C 10.9% intergenic (‑53/+127) PP_2167 ← / ← tal PhoD‑like phosphatase/transaldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,474,1480TC10.9% 95.0 / 2.8 38intergenic (‑53/+127)PP_2167/talPhoD‑like phosphatase/transaldolase
Reads supporting (aligned to +/- strand):  ref base T (15/18);  new base C (2/2);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GGTAGTGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATC  >  NC_002947/2474003‑2474293
                                                                                                                                                 |                                                                                                                                                 
ggTAGTGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGa                                                                                                                                                <  3:63502/149‑1 (MQ=255)
 gTAGTGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGAt                                                                                                                                               <  2:65722/149‑1 (MQ=255)
 gTAGTGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGAt                                                                                                                                               <  3:154710/149‑1 (MQ=255)
              agacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCGTACATAGATCAGTGTAGGAGCg                                                                                                                                  >  3:334218/1‑149 (MQ=255)
               gacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCgg                                                                                                                                 >  3:298201/1‑149 (MQ=255)
                acggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCggg                                                                                                                                <  5:207180/149‑1 (MQ=255)
                  ggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCGTACATCGATAAGTGTTGGGGCGGGCt                                                                                                                              >  3:93175/1‑149 (MQ=255)
                   gccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCtt                                                                                                                             <  2:231051/149‑1 (MQ=37)
                   gccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCtt                                                                                                                             <  8:50151/149‑1 (MQ=255)
                     cagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGGCCGTACCTCGCTGAGGGTAGGAGCGGGCTTgg                                                                                                                           >  5:152586/1‑148 (MQ=255)
                          gCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCCGGCAACCCCGCCCGGAAAAGGCTCAGGGTAGGCGCCGGCTTGGCcgcg                                                                                                                      >  3:158370/1‑149 (MQ=255)
                           cTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTGACATCGATCAGTGTAGGAGCGGGCTTGCCcgcg                                                                                                                      >  5:267156/1‑148 (MQ=255)
                                gTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAgg                                                                                                                <  2:102748/149‑1 (MQ=255)
                                     aGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGCTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCga                                                                                                           <  6:324075/149‑1 (MQ=255)
                                            gcAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGc                                                                                                                           <  4:71380/126‑1 (MQ=21)
                                            gcAGTACCGGGCCGGCGAGAACCAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGc                                                                                                                           >  3:71380/1‑126 (MQ=21)
                                                           cGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAg                                                                                       >  1:78265/1‑147 (MQ=255)
                                                           cGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAg                                                                                       <  2:78265/147‑1 (MQ=255)
                                                                  gAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGca                                                                               >  5:188875/1‑148 (MQ=255)
                                                                    ggggCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTTTTAGCCGGCAAACCCCCCCATACATAGATAAATTTATGAGCCGGCTTTACCCCGAACAGGCCCGAGAAATAAATACACAAAAAAAGCCTTGcaaa                                                                             >  4:318103/1‑148 (MQ=255)
                                                                                   gggAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGaaaa                                                             <  6:188875/149‑1 (MQ=255)
                                                                                        gtaaTCGACAGCTCGGGAGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCGCTTACATCGATCAGTGTAGGTGCGGGCTTGGCCGCTAACAGGCCAGAGAATTCATTACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTg                                                        <  3:155314/146‑1 (MQ=38)
                                                                                         tCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGa                                                       <  3:158943/149‑1 (MQ=255)
                                                                                          cATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGaa                                                      <  6:217908/149‑1 (MQ=255)
                                                                                                  gCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTccc                                              >  5:150283/1‑149 (MQ=255)
                                                                                                                    gcCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACg                            <  6:152586/149‑1 (MQ=255)
                                                                                                                       tGTTCGCGGGCAAACCCGCCCATACATCGAAAAATGTAAGAACAGGCTTGACCGCGAACAAGCCCGAGAAATAAATACACAACAAAGGCGTGGCAAACAAAACGGGCCGAAAATACTGAAAACTTCACCCCCCTCAGGCAAACACGa                           >  8:55462/1‑147 (MQ=255)
                                                                                                                         ttCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACt                        <  1:210468/148‑1 (MQ=255)
                                                                                                                           cgcgGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGt                      >  2:306908/1‑148 (MQ=255)
                                                                                                                              gggCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCa                  <  4:93175/149‑1 (MQ=255)
                                                                                                                                    gcccgctcCTACACTGATCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGAAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAACAACGAACTGTATCAGGCCtg            >  8:170346/1‑148 (MQ=25)
                                                                                                                                    gcccgcccTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtt            >  3:107802/1‑149 (MQ=255)
                                                                                                                                       ctcccTTACATCTATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTc         <  3:318103/147‑1 (MQ=255)
                                                                                                                                            ttACATCGATCAGTGTAGGAGCGGGCTTGCCAGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAGTACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTa    >  4:319178/1‑149 (MQ=255)
                                                                                                                                              aCATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTAt   >  2:174934/1‑148 (MQ=255)
                                                                                                                                               cATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATc  <  4:298201/148‑1 (MQ=255)
                                                                                                                                                aTCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCa                  <  3:369570/131‑1 (MQ=255)
                                                                                                                                                aTCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCa                  >  4:369570/1‑131 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGTAGTGACGAGCCAGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATC  >  NC_002947/2474003‑2474293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑