Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,474,160 G→A 13.6% intergenic (‑65/+115) PP_2167 ← / ← tal PhoD‑like phosphatase/transaldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,474,1600GA13.6% 84.3 / 5.0 37intergenic (‑65/+115)PP_2167/talPhoD‑like phosphatase/transaldolase
Reads supporting (aligned to +/- strand):  ref base G (16/15);  new base A (3/2);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

AGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCT  >  NC_002947/2474017‑2474306
                                                                                                                                               |                                                                                                                                                  
agacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCGTACATAGATCAGTGTAGGAGCg                                                                                                                                               >  3:334218/1‑149 (MQ=255)
 gacggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCgg                                                                                                                                              >  3:298201/1‑149 (MQ=255)
  acggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCggg                                                                                                                                             <  5:207180/149‑1 (MQ=255)
    ggccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCGTACATCGATAAGTGTTGGGGCGGGCt                                                                                                                                           >  3:93175/1‑149 (MQ=255)
     gccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCtt                                                                                                                                          <  2:231051/149‑1 (MQ=37)
     gccagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCtt                                                                                                                                          <  8:50151/149‑1 (MQ=255)
       cagacgCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGGCCGTACCTCGCTGAGGGTAGGAGCGGGCTTgg                                                                                                                                        >  5:152586/1‑148 (MQ=255)
            gCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCCGGCAACCCCGCCCGGAAAAGGCTCAGGGTAGGCGCCGGCTTGGCcgcg                                                                                                                                   >  3:158370/1‑149 (MQ=255)
             cTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTGACATCGATCAGTGTAGGAGCGGGCTTGCCcgcg                                                                                                                                   >  5:267156/1‑148 (MQ=255)
                  gTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAgg                                                                                                                             <  2:102748/149‑1 (MQ=255)
                       aGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGCTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCga                                                                                                                        <  6:324075/149‑1 (MQ=255)
                              gcAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGc                                                                                                                                        <  4:71380/126‑1 (MQ=21)
                              gcAGTACCGGGCCGGCGAGAACCAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCTCCTACACTGATCGATGTAAGGGCGGGCTTGc                                                                                                                                        >  3:71380/1‑126 (MQ=21)
                                             cGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAg                                                                                                    >  1:78265/1‑147 (MQ=255)
                                             cGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAg                                                                                                    <  2:78265/147‑1 (MQ=255)
                                                    gAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTAGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGca                                                                                            >  5:188875/1‑148 (MQ=255)
                                                      ggggCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTTTTAGCCGGCAAACCCCCCCATACATAGATAAATTTATGAGCCGGCTTTACCCCGAACAGGCCCGAGAAATAAATACACAAAAAAAGCCTTGcaaa                                                                                          >  4:318103/1‑148 (MQ=255)
                                                                     gggAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGaaaa                                                                          <  6:188875/149‑1 (MQ=255)
                                                                          gtaaTCGACAGCTCGGGAGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCGCTTACATCGATCAGTGTAGGTGCGGGCTTGGCCGCTAACAGGCCAGAGAATTCATTACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTg                                                                     <  3:155314/146‑1 (MQ=38)
                                                                           tCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGa                                                                    <  3:158943/149‑1 (MQ=255)
                                                                            cATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGaa                                                                   <  6:217908/149‑1 (MQ=255)
                                                                                    gCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTccc                                                           >  5:150283/1‑149 (MQ=255)
                                                                                                      gcCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACg                                         <  6:152586/149‑1 (MQ=255)
                                                                                                         tGTTCGCGGGCAAACCCGCCCATACATCGAAAAATGTAAGAACAGGCTTGACCGCGAACAAGCCCGAGAAATAAATACACAACAAAGGCGTGGCAAACAAAACGGGCCGAAAATACTGAAAACTTCACCCCCCTCAGGCAAACACGa                                        >  8:55462/1‑147 (MQ=255)
                                                                                                           ttCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACt                                     <  1:210468/148‑1 (MQ=255)
                                                                                                             cgcgGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGt                                   >  2:306908/1‑148 (MQ=255)
                                                                                                                gggCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCa                               <  4:93175/149‑1 (MQ=255)
                                                                                                                      gcccgctcCTACACTGATCGATGTAAGGGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGAAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAACAACGAACTGTATCAGGCCtg                         >  8:170346/1‑148 (MQ=25)
                                                                                                                      gcccgcccTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCtt                         >  3:107802/1‑149 (MQ=255)
                                                                                                                         ctcccTTACATCTATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTc                      <  3:318103/147‑1 (MQ=255)
                                                                                                                              ttACATCGATCAGTGTAGGAGCGGGCTTGCCAGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAGTACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTa                 >  4:319178/1‑149 (MQ=255)
                                                                                                                                aCATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTAt                >  2:174934/1‑148 (MQ=255)
                                                                                                                                 cATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATc               <  4:298201/148‑1 (MQ=255)
                                                                                                                                  aTCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCa                               >  4:369570/1‑131 (MQ=255)
                                                                                                                                  aTCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCa                               <  3:369570/131‑1 (MQ=255)
                                                                                                                                          gtgtAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTtctc       >  2:86599/1‑147 (MQ=255)
                                                                                                                                             tAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCt  >  2:179188/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
AGACGGCCAGACGCTGGGGTTCCAGGCGGCGCAGTACCGGGCCGGCGAGAACGAGGGGCAACGGGTTAGGGGAGGTCATCGACAGCTCGGGTGGCTATGGCGGCCTGTTCGCGGGCAAGCCCGCCCTTACATCGATCAGTGTAGGAGCGGGCTTGCCCGCGAACAGGCCCGAGAATTCAATACACAACTAAGGCGTGGCAAACAAAACGGGCGGAAAATACTGAACACTTCCCGCCCGTCAGGCAAACACGAACTGTATCAGGCCTTTTCAGCTATCAACTTCTCAAGCT  >  NC_002947/2474017‑2474306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑