Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,475,283 C→A 9.2% intergenic (‑82/+75) tal ← / ← dusA transaldolase/tRNA dihydrouridine synthase DusA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,475,2830CA 9.2% 188.9 / 7.2 62intergenic (‑82/+75)tal/dusAtransaldolase/tRNA dihydrouridine synthase DusA
Reads supporting (aligned to +/- strand):  ref base C (27/29);  new base A (2/4);  total (29/33)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCCAGGTCCCCGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAATGTCTGCC  >  NC_002947/2475136‑2475430
                                                                                                                                                   |                                                                                                                                                   
gTCCAGGTCCCCGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCg                                                                                                                                                    >  5:237929/1‑149 (MQ=255)
   cAGGTCCCCGGTGTCGGCGACCACGGTGGTTAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTg                                                                                                                                                 <  7:1741/149‑1 (MQ=255)
    aGGTCCCCGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTgg                                                                                                                                                >  3:13559/1‑149 (MQ=255)
     ggTCCCCGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGc                                                                                                                                               <  1:6242/149‑1 (MQ=255)
           cGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGca                                                                                                                                          >  7:96559/1‑148 (MQ=255)
            ggTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGGCCAGCGGGTCCGcagg                                                                                                                                        <  7:278992/149‑3 (MQ=255)
            ggTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGGCCAGCGGGTCCGcagg                                                                                                                                        <  3:61561/149‑3 (MQ=255)
             gtgtCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAg                                                                                                                                       <  1:333181/149‑1 (MQ=255)
                          cGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAg                                                                                                                          <  8:96559/149‑1 (MQ=255)
                          cGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAg                                                                                                                          <  1:211660/149‑1 (MQ=255)
                                   aCTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGAtt                                                                                                                 <  2:69296/149‑1 (MQ=255)
                                     tGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTc                                                                                                                >  8:183105/1‑148 (MQ=255)
                                       cTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGGCCAGCGGGTCCGcagg                                                                                                                                        >  8:159535/1‑120 (MQ=255)
                                       cTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGGCCAGCGGGTCCGcagg                                                                                                                                        <  7:159535/122‑3 (MQ=255)
                                       cTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAg                                                                                                             >  2:216111/1‑149 (MQ=255)
                                              tgttCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGCGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCgcaa                                                                                                       <  8:223029/148‑1 (MQ=255)
                                              tgttCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACACGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCgcaa                                                                                                       <  4:275268/148‑1 (MQ=255)
                                                  ccAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCgcaagcaa                                                                                                   <  1:40824/148‑1 (MQ=255)
                                                    aGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACACGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTCCGGACCCGCTGCCCCGCACAGGGCTGTACGAAAGTTGAAGATTAGAGACgcaagcaacac                                                                                                >  4:67529/1‑146 (MQ=255)
                                                             gTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTtcc                                                                                       >  4:115524/1‑149 (MQ=255)
                                                              tCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTtccc                                                                                      <  1:216111/149‑1 (MQ=255)
                                                                aTGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGTAAGCCCGAGGTtccct                                                                                     <  7:121955/148‑1 (MQ=255)
                                                                       tGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGa                                                                             <  2:91756/149‑1 (MQ=255)
                                                                       tGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGa                                                                             <  6:237929/149‑1 (MQ=255)
                                                                         ctctGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAg                                                                            <  7:95611/148‑1 (MQ=255)
                                                                         ctctGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAg                                                                            <  7:183105/148‑1 (MQ=255)
                                                                                   tgCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGccc                                                                  <  3:324347/148‑1 (MQ=255)
                                                                                   tgCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGccc                                                                  <  4:13559/148‑1 (MQ=255)
                                                                                      aTTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTg                                                               >  8:214243/1‑148 (MQ=255)
                                                                                       tttACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGc                                                              <  4:87097/148‑1 (MQ=255)
                                                                                       tttACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGc                                                              >  3:87097/1‑148 (MQ=255)
                                                                                           cTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTc                                                                                                                >  1:122058/1‑94 (MQ=255)
                                                                                           cTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTc                                                                                                                <  2:122058/94‑1 (MQ=255)
                                                                                            tCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCaacaac                                                        >  5:41844/1‑149 (MQ=255)
                                                                                              gACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAg                                                                                            <  8:155589/111‑1 (MQ=255)
                                                                                              gACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAg                                                                                            >  7:155589/1‑111 (MQ=255)
                                                                                              gACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCaacaac                                                        <  8:178218/147‑1 (MQ=255)
                                                                                              gACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTc                                                      >  6:343541/1‑149 (MQ=255)
                                                                                                cATTACACGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCa                                                    >  6:262872/1‑149 (MQ=255)
                                                                                                     cccGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGcc                                               <  1:351482/149‑1 (MQ=255)
                                                                                                      ccGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCt                                              >  3:83785/1‑149 (MQ=255)
                                                                                                      ccGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCt                                              >  8:77603/1‑149 (MQ=255)
                                                                                                      ccGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCt                                              >  5:212639/1‑149 (MQ=255)
                                                                                                       cGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTg                                             <  7:216641/149‑1 (MQ=255)
                                                                                                                       cTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAg                             >  3:72378/1‑149 (MQ=255)
                                                                                                                        tCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAgg                            >  8:117921/1‑149 (MQ=255)
                                                                                                                             ggCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCa                       <  7:117921/149‑1 (MQ=255)
                                                                                                                             ggCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCa                       <  3:115524/149‑1 (MQ=255)
                                                                                                                              gccgccGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAg                      >  1:210755/1‑149 (MQ=255)
                                                                                                                              gccgccGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAg                      >  1:204943/1‑149 (MQ=255)
                                                                                                                                       aCCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCCACCCCCGCCAAGGGGTCCGCCGCCTtgt             >  2:244261/1‑149 (MQ=255)
                                                                                                                                        ctgtGCGGGCCAGCGGGTCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTtgt             >  4:262917/4‑148 (MQ=255)
                                                                                                                                        cccTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTtgt             >  6:74077/1‑148 (MQ=255)
                                                                                                                                         ccTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTtgtg            >  6:173751/1‑148 (MQ=255)
                                                                                                                                          cTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGcaacaa                                                         <  7:171621/102‑1 (MQ=255)
                                                                                                                                          cTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGcaacaa                                                         >  8:171621/1‑102 (MQ=255)
                                                                                                                                          cTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGaa          >  4:331493/1‑149 (MQ=255)
                                                                                                                                          cTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGaa          >  4:326203/1‑149 (MQ=255)
                                                                                                                                             cGGGCCAGCGGGTCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAATGt       <  3:262917/149‑1 (MQ=255)
                                                                                                                                             cGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTTTGAAt         <  5:262872/147‑1 (MQ=255)
                                                                                                                                             cGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAAt         <  3:67529/147‑1 (MQ=255)
                                                                                                                                             cGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAATGt       <  7:214243/149‑1 (MQ=255)
                                                                                                                                                  ccGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAATGTCTGcc  >  4:24732/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTCCAGGTCCCCGGTGTCGGCGACCACGGTGGTGAACTGCTTGAGTTGTTCCAGCTTGGAGGTCATGGGCGTGCTCTGTCCTGTGCATTTACTCGACATTACCCGAGCCCCGGCAGCCGCTCAAGGGCCGCCGAAACCCTGCGGACCCGCTGGCCCGCACAGGGCTGTACGAAAGGTGAAGATTCGAGTCGCAAGCAAGCCCGAGGTTCCCTCCCTCTGAGGTCACCGCCCCTGCAACAACTCCACGGCCTGGTCAAACACCGCCAAGGGTTCAGCCGCCTTGTGAATGTCTGCC  >  NC_002947/2475136‑2475430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑