Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,582,224 G→A 6.2% A192A (GCG→GCA)  PP_2263 → sugar ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,582,2240GA 6.2% 191.5 / 2.1 65A192A (GCG→GCA) PP_2263sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (33/27);  new base A (2/2);  total (36/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

CCGCTGGCGGTGTGGATCCTGGAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGCTGGCGGCGGCGGGGGTGCTGA  >  NC_002947/2582078‑2582372
                                                                                                                                                     |                                                                                                                                                     
ccGCTGGCGGTGTGGATCCTGGAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGtt                                                                                                                                                     >  2:215550/1‑149 (MQ=255)
 cGCTGGCGGTGTGGATCCTGGAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATAGACGGCTACCGTTTTCCGCGCTTCTTCGTGAAGATCTTCCTACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGgtt                                                                                                                                                    >  3:279419/1‑149 (MQ=255)
   cTGGCGGTGTGGATCCTGGAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGttttt                                                                                                                                                  >  2:247442/1‑149 (MQ=255)
      gCGGTGTGGATCCTGGAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCt                                                                                                                                                <  3:17502/148‑1 (MQ=255)
             ggATCCTGGAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATg                                                                                                                                        >  1:15051/1‑149 (MQ=255)
              gATCCTGGAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATg                                                                                                                                        <  1:36089/148‑1 (MQ=255)
                   tGGAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑tctc                                                                                                                                  >  1:8286/1‑148 (MQ=255)
                   tGGAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTAAAGTTTTCAGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGGTTCATTG‑tctca                                                                                                                                 >  5:268475/1‑148 (MQ=255)
                     gAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑tctc                                                                                                                                  <  2:8286/146‑1 (MQ=255)
                     gAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTg                                                                                                                               >  6:234722/1‑149 (MQ=255)
                     gAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTg                                                                                                                               <  5:205832/149‑1 (MQ=255)
                         gTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTc                                                                                                                           <  6:63387/149‑1 (MQ=255)
                          tttCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGt                                                                                                                            <  2:358094/147‑1 (MQ=255)
                          tttCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGt                                                                                                                            >  1:358094/1‑147 (MQ=255)
                           ttCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCg                                                                                                                          >  8:10040/1‑148 (MQ=255)
                              aTGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTAGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAAct                                                                                                                      >  2:347924/1‑149 (MQ=255)
                                 tCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAActgct                                                                                                                   <  4:368326/149‑1 (MQ=255)
                                 tCGGGCGTCCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAActgct                                                                                                                   >  1:193351/1‑149 (MQ=255)
                                    ggCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGTCGATTTTCAGTTTCATGT‑TCTACTAGGTCGAActgctgct                                                                                                                >  6:233533/1‑149 (MQ=255)
                                            aaGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCa                                                                                                        >  4:105343/1‑149 (MQ=255)
                                               ggAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCAccc                                                                                                     >  2:125902/1‑149 (MQ=255)
                                                    tCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTGAAAGTCCGGCATCCGCGAGCACGGCTTTTTTATGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGCCGCCCAcc                                                                                                      >  1:115812/1‑146 (MQ=255)
                                                     cgacgaAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCt                                                                                               >  7:238540/1‑149 (MQ=255)
                                                        gcgatctgCCTACATCGACGGCTAGAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCg                                                                                             <  7:68449/141‑1 (MQ=255)
                                                         gaAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGt                                                                                           <  3:105343/149‑1 (MQ=255)
                                                             ccGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAAt                                                                                       <  7:10040/149‑1 (MQ=255)
                                                                ccTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCAAGGCGTTTTTCTGTTTCATGT‑TCTCCTGCGTCGAACTGCTGCTGGAGCCCAACATGACCTCg                                                                                             >  8:68449/1‑140 (MQ=255)
                                                                  tACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAAt                                                                                       <  4:283974/144‑1 (MQ=255)
                                                                  tACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAAt                                                                                       >  3:283974/1‑144 (MQ=255)
                                                                    cATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGc                                                                                <  2:15051/149‑1 (MQ=255)
                                                                    cATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGc                                                                                <  1:347924/149‑1 (MQ=255)
                                                                         aCGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGaa                                                                                        >  1:150978/1‑136 (MQ=255)
                                                                         aCGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGaa                                                                                        <  2:150978/136‑1 (MQ=255)
                                                                              tACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCt                                                                                               <  7:69115/124‑1 (MQ=255)
                                                                              tACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCt                                                                                               >  8:69115/1‑124 (MQ=255)
                                                                              tACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCcgc                                                                      >  6:27864/1‑149 (MQ=255)
                                                                                 aGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCATTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGt                                                                   >  2:182271/1‑149 (MQ=255)
                                                                                 aGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCATTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGt                                                                   >  2:176573/1‑149 (MQ=255)
                                                                                   ttttCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGtga                                                                 <  1:247442/149‑1 (MQ=255)
                                                                                     ttCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCgatgat                                                                <  5:27864/148‑1 (MQ=255)
                                                                                     ttCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGtgat                                                                <  5:234722/148‑1 (MQ=255)
                                                                                        cgcgCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGt                                                                                           >  1:347207/1‑118 (MQ=255)
                                                                                        cgcgCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGt                                                                                           <  2:347207/118‑1 (MQ=255)
                                                                                               cttcGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAAt                                                                                       >  4:94670/1‑115 (MQ=255)
                                                                                               cttcGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAAt                                                                                       <  3:94670/115‑1 (MQ=255)
                                                                                               cttcGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCAc                                                       <  2:193351/147‑1 (MQ=255)
                                                                                                    tGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCg                                                <  1:241601/149‑1 (MQ=255)
                                                                                                    tGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCg                                                >  4:302984/1‑149 (MQ=255)
                                                                                                    tGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCg                                                >  4:245200/1‑149 (MQ=255)
                                                                                                    tGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCAACGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCATGGGTCGAACTGCTGCTGGCGCGCCCCCTGACGTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCAGCGTGTCg                                                >  4:74156/1‑149 (MQ=255)
                                                                                                     gAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCgg                                               >  2:270122/1‑149 (MQ=255)
                                                                                                     gAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGCCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCgg                                               >  4:189705/1‑149 (MQ=255)
                                                                                                       aGATCTTCATACCGCTGCTTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTCCCGCGGTGATGACCCGCACGGAGTCggc                                              <  5:233533/148‑1 (MQ=255)
                                                                                                        gATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGACGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCggcat                                            >  1:78988/1‑149 (MQ=255)
                                                                                                              cATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCggcatcgg                                         <  1:215550/146‑1 (MQ=255)
                                                                                                                        aTTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTgggg                            >  8:78521/1‑149 (MQ=255)
                                                                                                                              gCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCACGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGtgtg                        >  2:228096/1‑149 (MQ=255)
                                                                                                                               cTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGc                       >  6:94973/1‑149 (MQ=255)
                                                                                                                                 ccGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGGCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGCTg                     <  5:76383/149‑1 (MQ=255)
                                                                                                                                  cGGCATCGGCG‑TCACGGCATTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGCTgg                    <  1:182271/149‑1 (MQ=255)
                                                                                                                                  cGGCATCGGCG‑TCACGGCATTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGCTgg                    <  1:176573/149‑1 (MQ=255)
                                                                                                                                   ggaacgggcg‑gcCCGGGGTTTTTTTTTTTAATTTCTCCCCTAGTTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGCTgg                    <  1:125902/143‑1 (MQ=255)
                                                                                                                                     cATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGCTggcgg                 >  6:290791/1‑149 (MQ=255)
                                                                                                                                         ggCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGCTggcggcgg              <  5:290791/148‑1 (MQ=255)
                                                                                                                                                  ggCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGCTGGCGGCGGCGGGGGTGc     >  2:242192/1‑149 (MQ=255)
                                                                                                                                                     gTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGCTGGCGGCGGCGGGGGTGCTGa  >  8:115815/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGCTGGCGGTGTGGATCCTGGAGGGTTTCATGTCGGGCGTGCCAAGGGAAATCGACGAAACCGCCTACATCGACGGCTACAGTTTTCCGCGCTTCTTCGTGAAGATCTTCATACCGCTGATTG‑‑GCTCCGGCATCGGCG‑TCACGGCGTTTTTCTGTTTCATGT‑TCTCCTGGGTCGAACTGCTGCTGGCGCGCACCCTGACCTCGGTGAATGCCAAGCCGATTGCCGCGGTGATGACCCGCACGGTGTCGGCATCGGGCATCGACTGGGGTGTGCTGGCGGCGGCGGGGGTGCTGA  >  NC_002947/2582078‑2582372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑