Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,650,091 T→A 13.5% intergenic (+30/+104) PP_2320 → / ← oprI hypothetical protein/major outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,650,0910TA13.5% 87.8 / 5.7 37intergenic (+30/+104)PP_2320/oprIhypothetical protein/major outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (15/17);  new base A (3/2);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

GGACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGT  >  NC_002947/2649944‑2650238
                                                                                                                                                   |                                                                                                                                                   
ggACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTa                                                                                                                                                    <  4:257546/149‑1 (MQ=255)
  aCATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGt                                                                                                                                                  >  4:362706/1‑149 (MQ=255)
  aCATGGTGCGTGACGTGGTGGCTGCAGAAGATGCTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACGAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGt                                                                                                                                                  <  7:74051/149‑1 (MQ=255)
     tGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCa                                                                                                                                               <  8:226398/149‑1 (MQ=255)
           gTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCACTAAGGGCAGGCCtg                                                                                                                                         <  2:212072/149‑2 (MQ=255)
            tGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCttt                                                                                                                                        <  7:232914/149‑1 (MQ=255)
                   gtggCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTAc                                                                                                                                  <  3:362706/148‑1 (MQ=255)
                                      tGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCg                                                                                                               <  6:154954/148‑1 (MQ=255)
                                           gTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTc                                                                                                          >  6:133692/1‑148 (MQ=255)
                                               aaaTTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAgg                                                                                                     <  2:159819/149‑1 (MQ=255)
                                                aaTTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGc                                                                                                    <  4:353080/149‑1 (MQ=255)
                                                        tgccgGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATaaaa                                                                                             >  3:215802/1‑148 (MQ=255)
                                                         gccgGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGACCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATaaaa                                                                                             >  6:234316/1‑147 (MQ=255)
                                                          ccgGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATaaaaaa                                                                                           <  1:221916/148‑1 (MQ=255)
                                                                gAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGAcc                                                                                    >  6:379111/1‑149 (MQ=255)
                                                                  gaaCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGc                                                                                                    >  4:238807/2‑131 (MQ=255)
                                                                  caaCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGc                                                                                                    <  3:238807/130‑1 (MQ=255)
                                                                        gggggCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCcac                                                                                 >  4:134310/1‑144 (MQ=255)
                                                                        gggggCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCcac                                                                                 <  3:134310/144‑1 (MQ=255)
                                                                                   cGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttct                                                                 <  5:133692/149‑1 (MQ=255)
                                                                                   cGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttct                                                                 <  5:379111/149‑1 (MQ=255)
                                                                                     aTGGTGACGAGCATTCCGCCCGAACTGCAGTAATGTAATTTCGGCGTACCCAGGACTGCCCTTAGTGCCGGCCTTTTCGTACCTGCTTACCGCGTTTGCCGGTTTCACAGGCAATAAAAAAGCCGACCAACAAGTGTGTCGGcttctt                                                                >  2:8610/1‑148 (MQ=255)
                                                                                                  ttCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGa                                                    <  7:128055/147‑1 (MQ=255)
                                                                                                  ttCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGa                                                    >  8:128055/1‑147 (MQ=255)
                                                                                                      gCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGatta                                              >  7:20455/1‑149 (MQ=255)
                                                                                                                      ggTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctggct                              <  8:20455/149‑1 (MQ=255)
                                                                                                                       gTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCtt                             >  4:188515/1‑149 (MQ=255)
                                                                                                                          aTTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTg                           <  4:215802/148‑1 (MQ=255)
                                                                                                                            ttCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTc                         >  7:331272/1‑148 (MQ=255)
                                                                                                                                     aaaaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGACGGTTTCAAAGTCAAT‑AAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGTATTATTACTTTCGGCTGGCTTTGTACAGCATACg                >  8:314698/4‑147 (MQ=255)
                                                                                                                                      ccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACg                >  6:30684/1‑147 (MQ=255)
                                                                                                                                       aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg             >  8:233481/2‑149 (MQ=255)
                                                                                                                                       aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg             >  8:295352/2‑149 (MQ=255)
                                                                                                                                       aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAAT‑AAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAgg            <  7:314698/148‑1 (MQ=255)
                                                                                                                                        aGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAgg            <  3:188515/149‑1 (MQ=255)
                                                                                                                                           ccTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAAAAATCCGTGAGGATTATTAATTGCGGATGGCTTTGTACAGCATACGAATGgcg         >  5:216900/1‑149 (MQ=255)
                                                                                                                                              gCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGgcgcg       >  4:223001/1‑148 (MQ=255)
                                                                                                                                                  ttAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGt  >  4:318599/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GGACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGT  >  NC_002947/2649944‑2650238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑