Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 220,619 T→C 7.5% intergenic (+76/‑52) PP_0168 → / → PP_5431 putative surface adhesion protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947220,6190TC 7.5% 154.7 / 3.1 54intergenic (+76/‑52)PP_0168/PP_5431putative surface adhesion protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (23/26);  new base C (2/2);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

GTCTAACCAGACGATCAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAGTTCACCGAAATG  >  NC_002947/220471‑220766
                                                                                                                                                    |                                                                                                                                                   
gTCTAACCAGACGATCAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCt                                                                                                                                                     >  5:156705/1‑149 (MQ=255)
   tAACCAGACGATCAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTgcg                                                                                                                                                  >  6:335845/1‑149 (MQ=255)
     aCCAGACGATCAACTCGTTGATCAGCGGCGCCTTTCCTACCTTCATCATCCACAACATGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTgcgc                                                                                                                                                 <  8:76904/148‑1 (MQ=255)
       cAGACGATCAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGcc                                                                                                                                              >  8:116440/1‑149 (MQ=255)
       cAGACGATCAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGATACCCGCCAACAGCGCATACACTGGCGCCGGGCCTGCGCGcc                                                                                                                                              >  7:211261/1‑149 (MQ=255)
        aGACGATCAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACGACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGccc                                                                                                                                             >  3:320742/1‑149 (MQ=255)
          aCGATCAACTGTTTTATCAGGGGCGCCTACCCGACCATCATCCTCCACAACATGGACCGCTGACACACCCCCGGGGCCGCACAGGGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGccc                                                                                                                                             <  2:312639/147‑1 (MQ=255)
                aaCTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCt                                                                                                                                     >  3:335261/1‑149 (MQ=255)
                    cGTTCATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGCCCACCGGGCCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGc                                                                                                                                 <  2:31037/149‑1 (MQ=255)
                         aTCAGCGGCGCCTATCCGACCAGCAGAATCCACAACAAGGACAGCTGCCAGACCACCGGGGCCGCACCGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTAtg                                                                                                                            <  8:278049/149‑1 (MQ=255)
                            aGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCCGGCCTGCGCGCCCCGCGCTGTTTGGCGAtgcgt                                                                                                                         >  6:32437/1‑147 (MQ=255)
                               ggcgCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGTCCTCACAGCGGCACCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACACTGGCGCCGGGCCTGCGCGCCCGGCGATCATTGCCTATGcttctg                                                                                                                      >  6:92331/1‑149 (MQ=255)
                                         cGACCATCATCATCCACAACATGGACTGCTGACAGACCACGGGGGCCGCAGTGGGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACcaaca                                                                                                            <  6:156705/149‑1 (MQ=255)
                                           aCCTTCATCATCCACAACAAGGACCGCTTACAGACCCCCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAg                                                                                                          <  4:314643/149‑1 (MQ=255)
                                                  tcatcCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAaacaa                                                                                                   >  4:151399/1‑149 (MQ=255)
                                                   aaaccACAACATGCCCCGCTCACCGACCCCGGGGCCCGAACCGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAaacaac                                                                                                  <  1:330342/146‑1 (MQ=255)
                                                    atcCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAaacaac                                                                                                  <  5:335845/148‑1 (MQ=255)
                                                    atcCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACg                                                                                                 <  5:130536/149‑1 (MQ=255)
                                                     tcCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGc                                                                                                                                 >  5:82702/1‑116 (MQ=255)
                                                     tcCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGt                                                                                                >  7:330304/1‑149 (MQ=255)
                                                      ccACAACAACCACCGCTCACCCCCCCCCGGGGCCGCACCGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTg                                                                                               <  5:32437/149‑1 (MQ=255)
                                                       caaaacaaGTACTGATTACAGACAACGGGTGCAGCAGTGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACATCGCATACTCTGGCGCCGGGCCTGCTCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTg                                                                                               <  5:92331/145‑1 (MQ=255)
                                                        acaacaAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCGGCGCGCCCGGCGATCTTTGCCTATGGTGCTGTCTACCACCCAGGAAACAACGTGAc                                                                                             >  8:148780/1‑149 (MQ=255)
                                                          aacaaGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACcaacaa                                                                                                           >  3:280039/1‑133 (MQ=255)
                                                           acaaGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAg                                                                                          <  5:374557/149‑1 (MQ=255)
                                                            caaGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAgg                                                                                         >  5:98185/1‑149 (MQ=255)
                                                                   aGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCg                                                                                  >  3:158500/1‑149 (MQ=255)
                                                                     cTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGcc                                                                                <  7:116440/149‑1 (MQ=255)
                                                                      tGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGc                                                                                 >  2:2421/1‑147 (MQ=255)
                                                                          aGACCCCCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGtt                                                                           <  2:78870/149‑1 (MQ=255)
                                                                           gACCACCGGTGCCGAACAGCGGCGCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACTACGTGTCGAGGGACATCGCCATGTTc                                                                          <  8:211261/149‑1 (MQ=255)
                                                                             ccaccGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTa                                                                        >  5:78663/1‑149 (MQ=255)
                                                                              caccGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTAc                                                                       <  1:81268/149‑1 (MQ=255)
                                                                                        gCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCCGCGATCTTTGCCTATGCTGCAGTCTACCAACAAGGAAACAACGTGACCAGGGTCATCGCCATGTTCTACGTgcaaacc                                                              >  3:170911/1‑145 (MQ=255)
                                                                                          acaGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTgcaacgcaac                                                           >  3:246319/1‑149 (MQ=255)
                                                                                                   ccGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTTCAAACAAGGAAACAACGTGACGAGGGACATCGCCATGTtataa                                                                       >  8:89438/1‑127 (MQ=255)
                                                                                                   ccGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGccgccgtc                                                  <  6:98185/149‑3 (MQ=255)
                                                                                                   ccGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCAGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTAc                                                                       <  7:89438/128‑1 (MQ=255)
                                                                                                          tttCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTtgc                                           >  7:210000/1‑149 (MQ=255)
                                                                                                             ccTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTtgctg                                         <  1:2421/148‑1 (MQ=255)
                                                                                                                 cTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCgg                                      <  4:246319/147‑1 (MQ=255)
                                                                                                                 cTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGt                                    <  4:158500/149‑1 (MQ=255)
                                                                                                                     ccGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGaa                                <  6:78663/149‑1 (MQ=255)
                                                                                                                      cGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGGGGCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAag                               <  4:170911/149‑1 (MQ=255)
                                                                                                                               cgcATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCgg                                      <  4:332993/133‑1 (MQ=255)
                                                                                                                               cgcATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCgg                                      >  3:332993/1‑133 (MQ=255)
                                                                                                                                 cATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGttt                    <  3:151399/149‑1 (MQ=255)
                                                                                                                                    caaagatcgcCGGGCGCGCAGGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGcc                          >  5:318279/8‑140 (MQ=255)
                                                                                                                                    caaagatcgcCGGGCGCGCAGGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGcc                          <  6:318279/133‑1 (MQ=255)
                                                                                                                                       ctGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAg              >  4:293077/1‑149 (MQ=255)
                                                                                                                                         atcgcCGGGCGCGCAGGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAg              <  4:189039/145‑1 (MQ=255)
                                                                                                                                         atcgcCGGGCGCGCAGGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAg              >  3:189039/3‑147 (MQ=255)
                                                                                                                                          gcgcCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAg              <  7:340380/146‑1 (MQ=255)
                                                                                                                                            gcCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAGTTCAc         >  5:318278/1‑149 (MQ=255)
                                                                                                                                                   cTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAGTTCACCGAAATg  >  8:104744/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTCTAACCAGACGATCAACTCGTTGATCAGCGGCGCCGATCCGACCATCATCATCCACAACAAGGACAGCTGACAGACCACCGGGGCCGCACAGCGGCCCCGGCTCTTTCCTGCTACCCGCCAACAGCGCATACTCTGGCGCCGGGCCTGCGCGCCCGGCGATCTTTGCCTATGCTGCTGTCTACCAACAAGGAAACAACGTGACGAGGGACATCGCCATGTTCTACGTGCAACGCAACGCCGCCGGCCAGTTGCTGCGGGTGGAAGCAGCCGCGTTTGATCAGTTCACCGAAATG  >  NC_002947/220471‑220766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑