Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,650,094 T→G 13.4% intergenic (+33/+101) PP_2320 → / ← oprI hypothetical protein/major outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,650,0940TG13.4% 87.3 / 3.7 35intergenic (+33/+101)PP_2320/oprIhypothetical protein/major outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (15/15);  new base G (3/2);  total (18/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGT  >  NC_002947/2649946‑2650238
                                                                                                                                                    |                                                                                                                                                
aCATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGt                                                                                                                                                  >  4:362706/1‑149 (MQ=255)
aCATGGTGCGTGACGTGGTGGCTGCAGAAGATGCTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACGAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGt                                                                                                                                                  <  7:74051/149‑1 (MQ=255)
   tGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCa                                                                                                                                               <  8:226398/149‑1 (MQ=255)
         gTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCACTAAGGGCAGGCCtg                                                                                                                                         <  2:212072/149‑2 (MQ=255)
          tGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCttt                                                                                                                                        <  7:232914/149‑1 (MQ=255)
                 gtggCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTAc                                                                                                                                  <  3:362706/148‑1 (MQ=255)
                                    tGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCg                                                                                                               <  6:154954/148‑1 (MQ=255)
                                         gTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTc                                                                                                          >  6:133692/1‑148 (MQ=255)
                                             aaaTTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAgg                                                                                                     <  2:159819/149‑1 (MQ=255)
                                              aaTTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGc                                                                                                    <  4:353080/149‑1 (MQ=255)
                                                      tgccgGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATaaaa                                                                                             >  3:215802/1‑148 (MQ=255)
                                                       gccgGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGACCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATaaaa                                                                                             >  6:234316/1‑147 (MQ=255)
                                                        ccgGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATaaaaaa                                                                                           <  1:221916/148‑1 (MQ=255)
                                                              gAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGAcc                                                                                    >  6:379111/1‑149 (MQ=255)
                                                                gaaCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGc                                                                                                    >  4:238807/2‑131 (MQ=255)
                                                                caaCCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGc                                                                                                    <  3:238807/130‑1 (MQ=255)
                                                                      gggggCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCcac                                                                                 >  4:134310/1‑144 (MQ=255)
                                                                      gggggCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCcac                                                                                 <  3:134310/144‑1 (MQ=255)
                                                                                 cGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttct                                                                 <  5:133692/149‑1 (MQ=255)
                                                                                 cGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGcttct                                                                 <  5:379111/149‑1 (MQ=255)
                                                                                   aTGGTGACGAGCATTCCGCCCGAACTGCAGTAATGTAATTTCGGCGTACCCAGGACTGCCCTTAGTGCCGGCCTTTTCGTACCTGCTTACCGCGTTTGCCGGTTTCACAGGCAATAAAAAAGCCGACCAACAAGTGTGTCGGcttctt                                                                >  2:8610/1‑148 (MQ=255)
                                                                                                ttCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGa                                                    <  7:128055/147‑1 (MQ=255)
                                                                                                ttCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGa                                                    >  8:128055/1‑147 (MQ=255)
                                                                                                    gCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGatta                                              >  7:20455/1‑149 (MQ=255)
                                                                                                                    ggTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCggctggct                              <  8:20455/149‑1 (MQ=255)
                                                                                                                     gTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCtt                             >  4:188515/1‑149 (MQ=255)
                                                                                                                        aTTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTg                           <  4:215802/148‑1 (MQ=255)
                                                                                                                          ttCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTc                         >  7:331272/1‑148 (MQ=255)
                                                                                                                                   aaaaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGACGGTTTCAAAGTCAAT‑AAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGTATTATTACTTTCGGCTGGCTTTGTACAGCATACg                >  8:314698/4‑147 (MQ=255)
                                                                                                                                    ccAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACg                >  6:30684/1‑147 (MQ=255)
                                                                                                                                     aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg             >  8:233481/2‑149 (MQ=255)
                                                                                                                                     aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAg             >  8:295352/2‑149 (MQ=255)
                                                                                                                                     aaGGCCTGCACTAAGGGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAAT‑AAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAgg            <  7:314698/148‑1 (MQ=255)
                                                                                                                                      aGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAgg            <  3:188515/149‑1 (MQ=255)
                                                                                                                                         ccTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAAAAATCCGTGAGGATTATTAATTGCGGATGGCTTTGTACAGCATACGAATGgcg         >  5:216900/1‑149 (MQ=255)
                                                                                                                                            gCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGgcgcg       >  4:223001/1‑148 (MQ=255)
                                                                                                                                                ttAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGt  >  4:318599/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ACATGGTGCGTGACGTGGTGGCTGCAGAAGATGGTATGCCGGTAGAAATTGCCGTGCCGGTGGAAAACCAGGGGGCCGCACCGATGGTGACGAGCATTCCGCCCGAACTGCAGTAAGGTAATTTCGGCGTACCCAGGCCTGCCCTTAGTGCAGGCCTTTTCGTACCTGCTGACCGCGTTTGCCGGTTTCAAAGGCAATAAAAAAGCCGACCCACAAGTGGGTCGGCTTCTTAACAATCCGTGAGGATTATTACTTGCGGCTGGCTTTGTCCAGCATACGCAGGGCGCGCTCGT  >  NC_002947/2649946‑2650238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑