Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,686,628 C→T 5.5% G365D (GGC→GAC)  PP_2354 ← two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,686,6280CT 5.5% 221.2 / 2.4 73G365D (GGC→GAC) PP_2354two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (34/34);  new base T (2/2);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

CGCTCGCGCTCGTTGATCTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGTTCGCGTGAGAAGCCCAG  >  NC_002947/2686481‑2686776
                                                                                                                                                   |                                                                                                                                                     
cgctcgcgctcgTTGATCTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGgc                                                                                                                                                       <  5:24259/148‑1 (MQ=255)
   tcgcgctcgTTGATCTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGgccgcc                                                                                                                                                   >  3:169245/1‑149 (MQ=255)
     gcgctcgTTGATCTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑gg                                                                                                                                                <  4:272251/149‑1 (MQ=255)
     gcgctcgTTGATCTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑gg                                                                                                                                                >  8:31987/1‑149 (MQ=255)
     gcgctcgTTGATCTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑gg                                                                                                                                                >  4:47449/1‑149 (MQ=255)
         tcgTTGATCTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATg                                                                                                                                            >  7:129432/1‑149 (MQ=255)
           gTTGATCTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATg                                                                                                                                            >  2:353376/1‑147 (MQ=255)
           gTTGATCTGTTCGCGCAGCAGCGCGTTGGCGTCTGACAGGCTCAGGGCGGATTCGCGCTCGCTGGTGATGTCGCGTGACACCACGTAAAGAAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCGGTTGTACGCC‑GGCATg                                                                                                                                            >  2:358963/1‑147 (MQ=255)
                 cTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGTCGCC‑GGCATGCAGGATGc                                                                                                                                    >  3:234440/1‑149 (MQ=255)
                 cTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGTCGCC‑GGCATGCAGGATGc                                                                                                                                    >  3:239149/1‑149 (MQ=255)
                  tGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCg                                                                                                                                   >  7:25948/1‑149 (MQ=255)
                   gTTCGCGCAGCCGCGCGTTGGCGGCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCgg                                                                                                                                  <  8:330976/149‑1 (MQ=255)
                    ttCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCg                                                                                                                                   <  7:31987/147‑1 (MQ=255)
                    ttCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGGGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCgg                                                                                                                                  <  2:325668/148‑1 (MQ=255)
                      agcgcAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGcc                                                                                                                                <  6:315301/147‑1 (MQ=255)
                           aGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGACGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACaaa                                                                                                                          <  2:98013/149‑1 (MQ=255)
                               gcgcgTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGGGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAgcgc                                                                                                                      <  6:5190/149‑1 (MQ=255)
                                       gCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGGGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGt                                                                                                              <  4:254466/149‑1 (MQ=255)
                                         gTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGACCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGGC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGc                                                                                                            <  2:216448/149‑1 (MQ=255)
                                              ccAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCgg                                                                                                          <  1:314517/146‑1 (MQ=255)
                                                aGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATg                                                                                                     >  2:242484/1‑149 (MQ=255)
                                                    tCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGc                                                                                                  >  4:205243/1‑148 (MQ=255)
                                                       gggCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAgg                                                                                              <  3:170432/149‑1 (MQ=255)
                                                           ggcTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGggcgg                                                                                          <  3:205243/149‑1 (MQ=255)
                                                            gcTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGggcggc                                                                                         >  4:273121/1‑149 (MQ=255)
                                                                cgcgCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAg                                                                                      <  3:47449/148‑1 (MQ=255)
                                                                    ctcgctGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCCCCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGGGGCCAGTTCgg                                                                                 >  6:313535/1‑149 (MQ=255)
                                                                     tcgctGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGt                                                                                <  8:129432/149‑1 (MQ=255)
                                                                     tcgctGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGt                                                                                <  4:202544/149‑1 (MQ=255)
                                                                       gctGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGt                                                                                <  5:173210/147‑1 (MQ=255)
                                                                       gctGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGt                                                                                <  3:273121/147‑1 (MQ=255)
                                                                       gctGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTg                                                                               <  3:14592/148‑1 (MQ=255)
                                                                           gTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGt                                                                            <  8:197306/147‑1 (MQ=255)
                                                                           gTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTaa                                                                          >  2:372447/1‑149 (MQ=255)
                                                                           gTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTaa                                                                          >  6:160658/1‑149 (MQ=255)
                                                                                    cgTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGTCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTg                                                                 <  4:239149/149‑1 (MQ=255)
                                                                                    cgTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGTCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTg                                                                 <  4:234440/149‑1 (MQ=255)
                                                                                       gCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCACGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTc                                                              <  1:289955/149‑1 (MQ=255)
                                                                                           ccacGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAACGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGc                                                          >  2:346294/1‑149 (MQ=255)
                                                                                               gTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAg                                                      <  1:372447/149‑1 (MQ=255)
                                                                                               gTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAg                                                      >  1:254228/1‑149 (MQ=255)
                                                                                               gTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATACGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGGGAGGGCGGCCAGTTCGGTGAGTCACTGTTCCTGGTCGTGCTGAg                                                      >  8:263287/1‑149 (MQ=255)
                                                                                                tACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAgg                                                     >  1:215292/1‑149 (MQ=255)
                                                                                                tACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGCTCCTGGTCGTGCTCAgg                                                     <  4:46770/149‑1 (MQ=255)
                                                                                                   aGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCAGGTGCGGCCATGGGCGAGGGCGGCCAGGTCGGTGAGTAACTGATCCAGGTCGTGCTCAGgcag                                                  >  4:364885/1‑149 (MQ=255)
                                                                                                    gCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGgcag                                                  >  2:309216/1‑148 (MQ=255)
                                                                                                     cAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGgcag                                                  >  1:36193/1‑147 (MQ=255)
                                                                                                     cAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGgcag                                                  <  2:36193/147‑1 (MQ=255)
                                                                                                         gtgtCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCCGGGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGt                                             >  1:275485/1‑149 (MQ=255)
                                                                                                           gtCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTg                                            >  6:287491/1‑147 (MQ=255)
                                                                                                              ctccGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTgcagc                                        >  6:172302/1‑148 (MQ=255)
                                                                                                                ccGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAag                                     >  8:306237/1‑149 (MQ=255)
                                                                                                                 cGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAag                                     >  5:14858/1‑148 (MQ=255)
                                                                                                                  ggCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAagga                                   <  4:169245/149‑1 (MQ=255)
                                                                                                                  ggCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAagga                                   <  6:218104/149‑1 (MQ=255)
                                                                                                                  ggCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAag                                     >  8:190427/1‑147 (MQ=255)
                                                                                                                   gCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAaggag                                  <  2:254228/149‑1 (MQ=255)
                                                                                                                   gCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAaggag                                  <  1:353376/149‑1 (MQ=255)
                                                                                                                   gCACCACCAACCACGACAGACAGCGCTGTTGGCCGCC‑TGCATGCAGTATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAaggag                                  <  1:358963/149‑1 (MQ=255)
                                                                                                                      ccaccaccCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGAc                               >  1:191485/1‑149 (MQ=255)
                                                                                                                        accaccCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGTATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTg                             <  1:309216/149‑1 (MQ=255)
                                                                                                                          cacccacGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGc                           >  4:205162/1‑149 (MQ=255)
                                                                                                                             ccacGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCt                          >  2:154232/1‑147 (MQ=255)
                                                                                                                             ccacGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCt                          >  6:83808/1‑147 (MQ=255)
                                                                                                                              cacGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAg                       <  5:160658/149‑1 (MQ=255)
                                                                                                                                cGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGc                     <  6:176609/149‑1 (MQ=255)
                                                                                                                                  aCAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGt                   >  8:314716/1‑149 (MQ=255)
                                                                                                                                     gccagcGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGttag                >  4:59768/1‑147 (MQ=255)
                                                                                                                                      ccagcGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTaa                                                                          >  6:96966/1‑90 (MQ=255)
                                                                                                                                      ccagcGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTaa                                                                          <  5:96966/90‑1 (MQ=255)
                                                                                                                                      ccagcGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGTTcgc               <  7:348031/149‑1 (MQ=255)
                                                                                                                                      ccagcGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGTTcgc               >  5:171850/1‑149 (MQ=255)
                                                                                                                                        agcGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGTTCGCGt             >  2:24246/1‑149 (MQ=255)
                                                                                                                                          cgcTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGTTCGCGTga           <  5:83808/149‑1 (MQ=255)
                                                                                                                                               ttGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGTTCGCGTGAGAAGc      >  2:277679/1‑149 (MQ=255)
                                                                                                                                                  gccgcc‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGTTCGCGTGAGAAGccc    <  1:277679/148‑1 (MQ=255)
                                                                                                                                                   ccgcc‑gGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGTTCGCGTGAGAAGCCCAg  <  6:14858/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGCTCGCGCTCGTTGATCTGTTCGCGCAGCCGCGCGTTGGCGTCTGCCAGGCTCAGGGCGGCTTCGCGCTCGCTGGTGATGTCGCGTGCCACCACGTACAGCAAGGTGTCCTCCGGCACCACCACCCACGACAGCCAGCGCTGTTGGCCGCC‑GGCATGCAGGATGCGGCCGACAAAGCGCGCGCTGGTGCGGCCATGGGCGAGGGCGGCCAGTTCGGTGAGTAACTGTTCCTGGTCGTGCTCAGGCAGCAGGTGCAGCAAGGAGGACTGGCTCAGGCGTTCGCGTGAGAAGCCCAG  >  NC_002947/2686481‑2686776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑