Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,715,420	C→A	5.7%	A45A (GCC→GCA)	cti →	esterified fatty acid cis/trans isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,715,420	0	C	A	5.7%	223.9 / 2.6	70	A45A (GCC→GCA)	cti	esterified fatty acid cis/trans isomerase
Reads supporting (aligned to +/- strand):  ref base C (35/31);  new base A (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GACTTTCCGCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTACGACGCACACAGCG  >  NC_002947/2715274‑2715565                                                                                                                                                   |                                                                                                                                                      gACTTTCCGCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTg                                                                                                                                                     <  7:143722/149‑1 (MQ=255)        cGCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGc                                                                                                                                               <  8:221721/148‑1 (MQ=255)         gCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGTCAGGCCCACCAGTCGAGCCGGGGTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCAGGCCACGcac                                                                                                                                             <  7:159093/149‑3 (MQ=255)         gCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCt                                                                                                                                             >  2:170622/1‑149 (MQ=255)           acaTGGTGCATCGTATCCTTGCGGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGc                                                                                                                                           <  4:318574/149‑1 (MQ=255)                   cATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGAcgc                                                                                                                                   >  5:117167/1‑149 (MQ=255)                     tCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGAcgccg                                                                                                                                 >  1:90368/1‑149 (MQ=255)                         aTCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTg                                                                                                                             >  6:129727/1‑149 (MQ=255)                          tCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGc                                                                                                                            <  4:247338/149‑1 (MQ=255)                          tCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGc                                                                                                                            <  5:124468/149‑1 (MQ=255)                             ttGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAg                                                                                                                         >  8:147914/1‑149 (MQ=255)                             ttGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGCCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAg                                                                                                                         >  5:292180/1‑149 (MQ=255)                                  ggCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCaa                                                                                                                    <  6:193741/149‑1 (MQ=255)                                       cTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTg                                                                                                                >  8:132923/1‑148 (MQ=255)                                        ttCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTgg                                                                                                               >  6:169522/1‑148 (MQ=255)                                        ttCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCAGGCCACGcac                                                                                                                                             <  4:302796/118‑3 (MQ=255)                                        ttCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCAGGCCACGcac                                                                                                                                             >  3:302796/1‑116 (MQ=255)                                           gCCCTGCTCATCAGCGGCGCGGTATTCGGGCATGCCCCCCAGTCGAGCCCGGCTATTTCCTACACGCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAg                                                                                                           <  5:216303/149‑1 (MQ=255)                                             ccTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGcc                                                                                                         <  5:212690/149‑1 (MQ=255)                                             ccTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGcc                                                                                                         >  2:192261/1‑149 (MQ=255)                                              cTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGccc                                                                                                        >  7:72283/1‑149 (MQ=255)                                                        gcggcgCGGTATTCGGGCAGGCGCCCCAGTCGAGCCCGGCTATTTCCTACCCGCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGcgcg                                                                                            <  1:370578/149‑1 (MQ=255)                                                             gcgGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGcc                                                                                                         >  5:363025/1‑133 (MQ=255)                                                             gcgGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGcc                                                                                                         <  6:363025/133‑1 (MQ=255)                                                               ggTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTAcg‑‑cg                                                                                   <  1:170622/149‑1 (MQ=255)                                                                   ttCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGcgcg                                                                                            <  5:129831/138‑1 (MQ=255)                                                                   ttCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGcgcg                                                                                            >  6:129831/1‑138 (MQ=255)                                                                     cGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGc                                                                               >  1:148681/1‑147 (MQ=255)                                                                      gggCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAg                                                                            >  1:151026/1‑149 (MQ=255)                                                                         cAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCaa                                                                         <  6:117167/149‑1 (MQ=255)                                                                               ccccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGCCGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCATGGTccc                                                                   >  2:308728/1‑149 (MQ=255)                                                                               ccccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGc                                                                                                                            <  6:88566/96‑1 (MQ=255)                                                                               ccccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGc                                                                                                                            >  5:88566/1‑96 (MQ=255)                                                                                cccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCg                                                                  >  1:157755/1‑149 (MQ=255)                                                                                cccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCg                                                                  <  1:192261/149‑1 (MQ=255)                                                                                cccAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCg                                                                  >  7:76915/1‑149 (MQ=255)                                                                                  cAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGgt                                                                <  5:169522/149‑1 (MQ=255)                                                                                   aGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGgtg                                                               <  7:147914/149‑1 (MQ=255)                                                                                      cGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGACGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAACCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTAc                                                            <  2:303847/149‑1 (MQ=255)                                                                                           ccGGCTATTTCCTACACCCGTGACATTCAACCGATCTTAACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCAGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGAGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAggg                                                       >  4:232751/1‑149 (MQ=255)                                                                                            cGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGc                                                      >  7:179689/1‑149 (MQ=255)                                                                                              gCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGc                                                      >  5:244683/1‑147 (MQ=255)                                                                                                  tttCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCgg                                                >  5:194530/1‑149 (MQ=255)                                                                                                    tCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGa                                               <  2:157755/148‑1 (MQ=255)                                                                                                     ccTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCgg                                                <  8:304918/146‑1 (MQ=255)                                                                                                     ccTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCgg                                                >  7:304918/1‑146 (MQ=255)                                                                                                      cTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGcc                                                                                                         <  1:5713/92‑1 (MQ=255)                                                                                                      cTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGcc                                                                                                         >  2:5713/1‑92 (MQ=255)                                                                                                      cTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGc                                             >  5:119017/1‑148 (MQ=255)                                                                                                       tACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGc                                             >  4:115052/1‑147 (MQ=255)                                                                                                            ccGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCgg                                                                                           <  2:351370/98‑1 (MQ=255)                                                                                                            ccGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCgg                                                                                           >  1:351370/1‑98 (MQ=255)                                                                                                             cGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGt                                     <  3:115052/149‑1 (MQ=255)                                                                                                                     tCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCccacca                             >  7:112503/1‑149 (MQ=255)                                                                                                                     tCAACCGAACATCACCGAGAAGTGCGTGCCCTGCCACGCCTGCAACCACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCAGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCccacca                             >  5:106657/1‑149 (MQ=255)                                                                                                                         ccGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCTGGAGTAGCCGCGAGACGGCCGGGGCCCCCAAGGACCCGGTGTACCAGGGGGAGCGGCGCAAGCCAGACCccaccc                             >  1:116483/1‑148 (MQ=255)                                                                                                                          cGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCgg                        >  1:54599/1‑149 (MQ=255)                                                                                                                             tCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTg                     >  2:266651/1‑149 (MQ=255)                                                                                                                                tCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTc                  >  8:323298/1‑149 (MQ=255)                                                                                                                                 cACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCt                      <  6:119017/144‑1 (MQ=255)                                                                                                                                 cACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGtt                   <  4:159759/147‑1 (MQ=255)                                                                                                                                  aCCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTa                <  2:151026/149‑1 (MQ=255)                                                                                                                                   ccGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTac               <  8:72283/149‑1 (MQ=255)                                                                                                                                      agaAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAgg                                         <  7:6112/120‑1 (MQ=255)                                                                                                                                      agaAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAgg                                         >  8:6112/1‑120 (MQ=255)                                                                                                                                        aaGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTacgacg           >  3:38652/1‑148 (MQ=255)                                                                                                                                          aggCGTGGCAGGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTACGCCGca         >  3:143206/3‑148 (MQ=255)                                                                                                                                           tGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTACGACGcaca       <  6:194530/149‑1 (MQ=255)                                                                                                                                             cGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTACGACGcacac      <  8:179689/148‑1 (MQ=255)                                                                                                                                                 gCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTACGACGCACACAGCg  <  5:129727/148‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                      GACTTTCCGCACATGGTGCATCGTATCCTTGCCGGCGCCTTCGCCCTGCTCATCAGCGGCGCGGTATTCGGGCAGGCCCCCCAGTCGAGCCCGGCTATTTCCTACACCCGGGACATTCAACCGATCTTCACCGAGAAGTGCGTGGCCTGCCACGCCTGCAACGACGCCGCCTGCCAGCTCAAGCTGGAAAGCCCTGA‑‑AGGCGCGGTACG‑‑CGGGGCCAGCAAGGTCCCGGTGTACCAGGGCGAGCGGAGCAAGGCAGTCCCCACCACGCGGCTGTTCTACGACGCACACAGCG  >  NC_002947/2715274‑2715565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑