Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,722,068 C→T 100% Q197* (CAG→TAG)  PP_2382 → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,722,0680CT100.0% 136.1 / NA 40Q197* (CAG→TAG) PP_2382putative transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/17);  total (23/17)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCTCGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAACAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTC  >  NC_002947/2721927‑2722212
                                                                                                                                             |                                                                                                                                                
gccTCGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCAtt                                                                                                                                           <  4:272293/149‑1 (MQ=255)
    cGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGAt                                                                                                                                       >  8:7061/1‑149 (MQ=255)
    cGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGAt                                                                                                                                       >  8:1593/1‑149 (MQ=255)
         cTCGGGCCGCTGGTCGTTGCGGCCTGTGTGTTACTGCTGTGTCGCGACGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCACTATGCATTCGCTGGGt                                                                                                                                   <  2:256014/148‑1 (MQ=255)
                     gTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATc                                                                                                                        <  8:338311/147‑1 (MQ=255)
                      tCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAgg                                                                                                                     <  7:1593/149‑1 (MQ=255)
                      tCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAgg                                                                                                                     >  5:303519/1‑149 (MQ=255)
                      tCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAgg                                                                                                                     <  6:303519/149‑1 (MQ=255)
                      tCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAgg                                                                                                                     <  7:7061/149‑1 (MQ=255)
                      tCTTTTCGGCCTGTGTGATACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGGCTTATGGTGTCGGCCATTTGTATAGCTACCTTGACGCCTGCCAGGTGTAGTGTCAAATAGGCATTAGATGGGTGCGCAATAATCAgg                                                                                                                     >  3:233386/1‑149 (MQ=255)
                        ttttCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAggg                                                                                                                    <  8:207915/148‑1 (MQ=255)
                         tttCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCa                                                                                                                                             >  4:38438/1‑122 (MQ=255)
                         tttCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCa                                                                                                                                             <  3:38438/122‑1 (MQ=255)
                         tttCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGAt                                                                                                                  >  1:243835/1‑149 (MQ=255)
                                   tgtgtTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCggg                                                                                                          <  8:177397/147‑1 (MQ=255)
                                       ttACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAgtgt                                                                                                    >  3:292308/1‑149 (MQ=255)
                                                  tcGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGa                                                                                         >  8:223511/1‑149 (MQ=255)
                                                    gagaAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAgg                                                                                       >  4:269215/1‑149 (MQ=255)
                                                        aGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCg                                                                                   <  5:380641/149‑1 (MQ=255)
                                                                     ttttttGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCgtt                                                                      <  4:68135/149‑1 (MQ=255)
                                                                           gCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGc                                                                >  5:370603/1‑149 (MQ=255)
                                                                            cAATGTGCCTTATCGTGGCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCt                                                               <  4:292308/149‑1 (MQ=255)
                                                                             aaTGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTg                                                              >  5:50697/1‑149 (MQ=255)
                                                                                            gtCGGCCATTTGTATCGCTACCTTGACGCCTGCGAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGGCGGTGTTCGCTGTCATTGGCTGcacca                                               >  2:100229/1‑149 (MQ=255)
                                                                                               ggCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACcaca                                             >  2:142327/1‑148 (MQ=255)
                                                                                               ggCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCacac                                            >  2:145223/1‑149 (MQ=255)
                                                                                               ggCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCacac                                            >  2:205220/1‑149 (MQ=255)
                                                                                                gCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACAct                                           >  2:342483/1‑149 (MQ=255)
                                                                                                        tATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGa                                   >  4:204769/1‑149 (MQ=255)
                                                                                                          tCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATg                                 >  1:240376/1‑149 (MQ=255)
                                                                                                           cGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGt                                >  1:88919/1‑149 (MQ=255)
                                                                                                             cTACCTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGTGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTc                              <  1:100229/149‑1 (MQ=255)
                                                                                                                ccTTGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGcc                           <  6:50697/149‑1 (MQ=255)
                                                                                                                   tGACGCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACg                        >  8:67861/1‑149 (MQ=255)
                                                                                                                       gCCTGCCAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCgg                    <  3:269215/149‑1 (MQ=255)
                                                                                                                             cAGGTGTAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTc              <  1:205220/149‑1 (MQ=255)
                                                                                                                                gtgtAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCt           >  1:147322/1‑149 (MQ=255)
                                                                                                                                   tAGTGTCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTa        >  3:83455/1‑149 (MQ=255)
                                                                                                                                     gtgtCCAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATc      >  3:182681/1‑149 (MQ=255)
                                                                                                                                          cAATAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTc  <  2:240376/148‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                
GCCTCGATCCTCGGGCCGCTGGTCTTTTCGGCCTGTGTGTTACTGCTGTCTCGAGAAGCCACTTTCTTGTTTTTTGCAATGTGCCTTATCGTGTCGGCCATTTGTATCGCTACCTTGACGCCTGCCAGGTGTAGTGTCCAACAGGCATTCGATGGGTGCGCCATAATCAGGGATGCGGCGGGCAGTGTGAGTCAGTTGAGGGCCGGCAATGCGCCGTTGTTCGCTGTCATTGGCTGCACCACACTCTGTGCGATGTTCGCCACGTCGGTGATTCGCTTTATCCTTC  >  NC_002947/2721927‑2722212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑