Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,804,782 C→G 6.1% R199G (CGC→GGC)  rbsR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,804,7820CG 6.1% 210.6 / 4.0 65R199G (CGC→GGC) rbsRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (37/24);  new base G (2/2);  total (39/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.13e-01

GGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGAT  >  NC_002947/2804635‑2804927
                                                                                                                                                   |                                                                                                                                                 
ggCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTccg                                                                                                                                                  <  3:19997/149‑1 (MQ=255)
    tCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGt                                                                                                                                              <  2:370912/149‑1 (MQ=255)
    tCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGt                                                                                                                                              <  5:355616/149‑1 (MQ=255)
       aTGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTccg                                                                                                                                                  >  1:227388/1‑142 (MQ=255)
       aTGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTccg                                                                                                                                                  <  2:227388/142‑1 (MQ=255)
          ccGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCTCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGt                                                                                                                                              <  6:270819/143‑1 (MQ=255)
          ccGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCACCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGt                                                                                                                                              >  5:270819/1‑143 (MQ=255)
           cGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATgg                                                                                                                                       <  8:247593/149‑1 (MQ=255)
            gACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGc                                                                                                                                      >  5:271133/1‑149 (MQ=255)
             aCCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGcc                                                                                                                                     <  5:111412/149‑1 (MQ=255)
               cTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGa                                                                                                                                   <  5:314081/149‑1 (MQ=255)
                  gTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGaa                                                                                                                                  <  8:306005/147‑1 (MQ=255)
                       cATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTg                                                                                                                           >  3:315582/1‑149 (MQ=255)
                       cATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTg                                                                                                                           <  3:282796/149‑1 (MQ=255)
                         ttGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCGGCCATGGCACGGCGGAAGCCgctcag                                                                                                                         <  6:151301/149‑6 (MQ=255)
                           gACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGTTgcgc                                                                                                                        <  4:280268/148‑1 (MQ=255)
                              cACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGt                                                                                                                    >  3:212858/1‑149 (MQ=255)
                                        gggCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGc                                                                                                          <  5:11664/149‑1 (MQ=255)
                                           cGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCgt                                                                                                       >  7:271522/1‑149 (MQ=255)
                                            gTACCTGGCCACCCGGCATCTGCTGGAGCTTGTCCATCGCGACGTCGCCTATAGCTGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCgtg                                                                                                      <  8:278698/149‑1 (MQ=255)
                                                          ggCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGAc                                                                                        >  2:164725/1‑149 (MQ=255)
                                                              tCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCa                                                                                    >  1:369562/1‑149 (MQ=255)
                                                              tCTGCTGGAGCTTGGCCATCGAGACGTCGCCTATATCGGCGGCCCAGCAGAAACAGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTc                                                                                     >  1:363894/1‑148 (MQ=255)
                                                                     gAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGcc                                                                              <  1:164725/148‑1 (MQ=255)
                                                                      aGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCg                                                                            >  7:249188/1‑149 (MQ=255)
                                                                      aGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCg                                                                            >  2:363235/1‑149 (MQ=255)
                                                                         ttGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGt                                                                         >  6:246605/1‑149 (MQ=255)
                                                                           ggCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTg                                                                        <  3:176094/148‑1 (MQ=255)
                                                                            gCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGc                                                                      >  2:115240/1‑149 (MQ=255)
                                                                             ccATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGcc                                                                     <  4:315582/149‑1 (MQ=255)
                                                                                       gTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggc                                                           <  8:271522/149‑1 (MQ=255)
                                                                                          gCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcggc                                                        <  1:115240/149‑1 (MQ=255)
                                                                                           ccggtttCGGCGGGGCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcgg                                                         <  7:253252/141‑1 (MQ=255)
                                                                                           ccggtttCGGCGGGGCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcgg                                                         >  8:253252/7‑147 (MQ=255)
                                                                                           ccTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcggcg                                                       >  1:151277/1‑149 (MQ=255)
                                                                                            cTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcggcg                                                       >  2:111631/1‑148 (MQ=255)
                                                                                              atatCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAg                                                    >  5:198737/1‑149 (MQ=255)
                                                                                                 tCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTg                                                 >  6:85840/1‑149 (MQ=255)
                                                                                                    gcggcCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTg                                              >  8:2156/1‑149 (MQ=255)
                                                                                                        ccccGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACGTCACCAGCCCGGGTGGCCAAGCAGCGGCGGCGCCGGTGCGGGAAg                                          >  7:312713/1‑149 (MQ=255)
                                                                                                         cccGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAgg                                         >  3:99641/1‑149 (MQ=255)
                                                                                                         cccGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCACCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAgg                                         >  1:71073/1‑149 (MQ=255)
                                                                                                          ccgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                           >  1:187717/1‑146 (MQ=255)
                                                                                                          ccgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                           <  2:187717/146‑1 (MQ=255)
                                                                                                          ccgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAgg                                         >  7:248226/1‑148 (MQ=255)
                                                                                                           cgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGc                                        >  6:345586/1‑148 (MQ=255)
                                                                                                            gccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGc                                        >  2:56085/1‑147 (MQ=255)
                                                                                                             ccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGc                                                <  4:187421/138‑1 (MQ=255)
                                                                                                             ccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGc                                                >  3:187421/1‑138 (MQ=255)
                                                                                                                    cGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGACGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCt                              >  1:76016/1‑149 (MQ=255)
                                                                                                                      gggTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCg                            >  4:229018/1‑149 (MQ=255)
                                                                                                                      gggTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCg                            <  5:246605/149‑1 (MQ=255)
                                                                                                                       ggTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCgg                           >  8:319091/1‑149 (MQ=255)
                                                                                                                             ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCACGCGGCCCTCGGCGAttt                     >  4:33581/1‑149 (MQ=255)
                                                                                                                             ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGc                                    <  6:88803/134‑1 (MQ=255)
                                                                                                                             ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGc                                    >  5:88803/1‑134 (MQ=255)
                                                                                                                             ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCgg                           <  6:345732/143‑1 (MQ=255)
                                                                                                                             ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCgg                           >  5:345732/1‑143 (MQ=255)
                                                                                                                             ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGAttt                     >  5:90398/1‑149 (MQ=255)
                                                                                                                                         caccGGCTTCGGCCATGGCACGGCGGAAGCCGCTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                           <  5:126644/112‑1 (MQ=37)
                                                                                                                                         caccGGCTTCGGCCATGGCACGGCGGAAGCCGCTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                           >  6:126644/4‑115 (MQ=37)
                                                                                                                                          accGGCTTCGGCCATGGCACGGCGGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGa        >  3:166814/3‑149 (MQ=255)
                                                                                                                                               cTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAg                                          >  3:277587/1‑110 (MQ=255)
                                                                                                                                               cTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAg                                          <  4:277587/110‑1 (MQ=255)
                                                                                                                                               cTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGa   >  5:194455/1‑149 (MQ=255)
                                                                                                                                                ttCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCgg              <  3:32929/137‑1 (MQ=255)
                                                                                                                                                ttCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCgg              >  4:32929/1‑137 (MQ=255)
                                                                                                                                                ttCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGAt  >  5:314577/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGCGTCGATGCCGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGAT  >  NC_002947/2804635‑2804927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑