Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,804,792 T→C 5.5% M202T (ATG→ACG)  rbsR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,804,7920TC 5.5% 219.1 / 2.3 72M202T (ATG→ACG) rbsRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base T (40/28);  new base C (2/2);  total (42/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCTTTGGTGTG  >  NC_002947/2804646‑2804940
                                                                                                                                                  |                                                                                                                                                    
cGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATgg                                                                                                                                                    <  8:247593/149‑1 (MQ=255)
 gACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGc                                                                                                                                                   >  5:271133/1‑149 (MQ=255)
  aCCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGcc                                                                                                                                                  <  5:111412/149‑1 (MQ=255)
    cTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGa                                                                                                                                                <  5:314081/149‑1 (MQ=255)
       gTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGaa                                                                                                                                               <  8:306005/147‑1 (MQ=255)
            cATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTg                                                                                                                                        <  3:282796/149‑1 (MQ=255)
            cATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTg                                                                                                                                        >  3:315582/1‑149 (MQ=255)
              ttGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCGGCCATGGCACGGCGGAAGCCgctcag                                                                                                                                      <  6:151301/149‑6 (MQ=255)
                gACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGTTgcgc                                                                                                                                     <  4:280268/148‑1 (MQ=255)
                   cACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGt                                                                                                                                 >  3:212858/1‑149 (MQ=255)
                             gggCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGc                                                                                                                       <  5:11664/149‑1 (MQ=255)
                                cGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCgt                                                                                                                    >  7:271522/1‑149 (MQ=255)
                                 gTACCTGGCCACCCGGCATCTGCTGGAGCTTGTCCATCGCGACGTCGCCTATAGCTGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCgtg                                                                                                                   <  8:278698/149‑1 (MQ=255)
                                               ggCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGAc                                                                                                     >  2:164725/1‑149 (MQ=255)
                                                   tCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCa                                                                                                 >  1:369562/1‑149 (MQ=255)
                                                   tCTGCTGGAGCTTGGCCATCGAGACGTCGCCTATATCGGCGGCCCAGCAGAAACAGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTc                                                                                                  >  1:363894/1‑148 (MQ=255)
                                                          gAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGcc                                                                                           <  1:164725/148‑1 (MQ=255)
                                                           aGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCg                                                                                         >  7:249188/1‑149 (MQ=255)
                                                           aGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCg                                                                                         >  2:363235/1‑149 (MQ=255)
                                                              ttGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGt                                                                                      >  6:246605/1‑149 (MQ=255)
                                                                ggCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTg                                                                                     <  3:176094/148‑1 (MQ=255)
                                                                 gCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGc                                                                                   >  2:115240/1‑149 (MQ=255)
                                                                  ccATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGcc                                                                                  <  4:315582/149‑1 (MQ=255)
                                                                            gTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggc                                                                        <  8:271522/149‑1 (MQ=255)
                                                                               gCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcggc                                                                     <  1:115240/149‑1 (MQ=255)
                                                                                ccggtttCGGCGGGGCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcgg                                                                      <  7:253252/141‑1 (MQ=255)
                                                                                ccggtttCGGCGGGGCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcgg                                                                      >  8:253252/7‑147 (MQ=255)
                                                                                ccTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcggcg                                                                    >  1:151277/1‑149 (MQ=255)
                                                                                 cTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcggcg                                                                    >  2:111631/1‑148 (MQ=255)
                                                                                   atatCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAg                                                                 >  5:198737/1‑149 (MQ=255)
                                                                                      tCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTg                                                              >  6:85840/1‑149 (MQ=255)
                                                                                         gcggcCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTg                                                           >  8:2156/1‑149 (MQ=255)
                                                                                             ccccGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACGTCACCAGCCCGGGTGGCCAAGCAGCGGCGGCGCCGGTGCGGGAAg                                                       >  7:312713/1‑149 (MQ=255)
                                                                                              cccGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAgg                                                      >  3:99641/1‑149 (MQ=255)
                                                                                              cccGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCACCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAgg                                                      >  1:71073/1‑149 (MQ=255)
                                                                                               ccgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                                        <  2:187717/146‑1 (MQ=255)
                                                                                               ccgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                                        >  1:187717/1‑146 (MQ=255)
                                                                                               ccgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAgg                                                      >  7:248226/1‑148 (MQ=255)
                                                                                                cgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGc                                                     >  6:345586/1‑148 (MQ=255)
                                                                                                 gccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGc                                                     >  2:56085/1‑147 (MQ=255)
                                                                                                  ccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGc                                                             <  4:187421/138‑1 (MQ=255)
                                                                                                  ccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGc                                                             >  3:187421/1‑138 (MQ=255)
                                                                                                         cGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGACGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCt                                           >  1:76016/1‑149 (MQ=255)
                                                                                                           gggTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCg                                         >  4:229018/1‑149 (MQ=255)
                                                                                                           gggTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCg                                         <  5:246605/149‑1 (MQ=255)
                                                                                                            ggTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCgg                                        >  8:319091/1‑149 (MQ=255)
                                                                                                                  ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCACGCGGCCCTCGGCGAttt                                  >  4:33581/1‑149 (MQ=255)
                                                                                                                  ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGc                                                 <  6:88803/134‑1 (MQ=255)
                                                                                                                  ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGc                                                 >  5:88803/1‑134 (MQ=255)
                                                                                                                  ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCgg                                        >  5:345732/1‑143 (MQ=255)
                                                                                                                  ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCgg                                        <  6:345732/143‑1 (MQ=255)
                                                                                                                  ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGAttt                                  >  5:90398/1‑149 (MQ=255)
                                                                                                                              caccGGCTTCGGCCATGGCACGGCGGAAGCCGCTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                                        >  6:126644/4‑115 (MQ=37)
                                                                                                                              caccGGCTTCGGCCATGGCACGGCGGAAGCCGCTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                                        <  5:126644/112‑1 (MQ=37)
                                                                                                                               accGGCTTCGGCCATGGCACGGCGGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGa                     >  3:166814/3‑149 (MQ=255)
                                                                                                                                    cTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAg                                                       >  3:277587/1‑110 (MQ=255)
                                                                                                                                    cTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAg                                                       <  4:277587/110‑1 (MQ=255)
                                                                                                                                    cTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGa                >  5:194455/1‑149 (MQ=255)
                                                                                                                                     ttCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCgg                           >  4:32929/1‑137 (MQ=255)
                                                                                                                                     ttCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCgg                           <  3:32929/137‑1 (MQ=255)
                                                                                                                                     ttCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGAt               >  5:314577/1‑149 (MQ=255)
                                                                                                                                           cgTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCtt         <  4:212858/149‑1 (MQ=255)
                                                                                                                                           cgTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCtt         <  6:314577/149‑1 (MQ=255)
                                                                                                                                            gTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCttt        <  5:85840/149‑1 (MQ=255)
                                                                                                                                            gTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCttt        >  1:17847/1‑149 (MQ=255)
                                                                                                                                             tGCCATGGCCGAAGCCGGTGCGCCCGTGCCTGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCTTTg       <  6:271133/149‑1 (MQ=255)
                                                                                                                                               ccATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCTTTGgt     <  2:17847/149‑1 (MQ=255)
                                                                                                                                               ccATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCTTTGgt     <  6:194455/149‑1 (MQ=255)
                                                                                                                                               ccATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCTTTGgt     <  2:71073/149‑1 (MQ=255)
                                                                                                                                                 aTGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCTTTGgtgt   >  8:142827/1‑149 (MQ=255)
                                                                                                                                                 aTGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCTTTGgtgt   >  6:13103/1‑149 (MQ=255)
                                                                                                                                                 aTGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCTTTGgtgt   >  1:325456/1‑149 (MQ=255)
                                                                                                                                                  tGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCTTTGGtgtg  >  3:357119/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTTTTGCCGGCAACGACATGATCGGCTTTGGTGTG  >  NC_002947/2804646‑2804940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑