Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,804,796 C→G 5.7% A203A (GCC→GCG)  rbsR → DNA‑binding transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,804,7960CG 5.7% 231.4 / 3.8 70A203A (GCC→GCG) rbsRDNA‑binding transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (40/26);  new base G (2/2);  total (42/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

ACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCTTTGGTGTGCT  >  NC_002947/2804648‑2804942
                                                                                                                                                    |                                                                                                                                                   
aCCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGcc                                                                                                                                                     <  5:111412/149‑1 (MQ=255)
  cTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGa                                                                                                                                                   <  5:314081/149‑1 (MQ=255)
     gTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGaa                                                                                                                                                  <  8:306005/147‑1 (MQ=255)
          cATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTg                                                                                                                                           >  3:315582/1‑149 (MQ=255)
          cATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTg                                                                                                                                           <  3:282796/149‑1 (MQ=255)
            ttGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCGGCCATGGCACGGCGGAAGCCgctcag                                                                                                                                         <  6:151301/149‑6 (MQ=255)
              gACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGTTgcgc                                                                                                                                        <  4:280268/148‑1 (MQ=255)
                 cACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGt                                                                                                                                    >  3:212858/1‑149 (MQ=255)
                           gggCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGc                                                                                                                          <  5:11664/149‑1 (MQ=255)
                              cGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCgt                                                                                                                       >  7:271522/1‑149 (MQ=255)
                               gTACCTGGCCACCCGGCATCTGCTGGAGCTTGTCCATCGCGACGTCGCCTATAGCTGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCgtg                                                                                                                      <  8:278698/149‑1 (MQ=255)
                                             ggCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGAc                                                                                                        >  2:164725/1‑149 (MQ=255)
                                                 tCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCa                                                                                                    >  1:369562/1‑149 (MQ=255)
                                                 tCTGCTGGAGCTTGGCCATCGAGACGTCGCCTATATCGGCGGCCCAGCAGAAACAGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTc                                                                                                     >  1:363894/1‑148 (MQ=255)
                                                        gAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGcc                                                                                              <  1:164725/148‑1 (MQ=255)
                                                         aGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCg                                                                                            >  2:363235/1‑149 (MQ=255)
                                                         aGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCg                                                                                            >  7:249188/1‑149 (MQ=255)
                                                            ttGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGt                                                                                         >  6:246605/1‑149 (MQ=255)
                                                              ggCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTg                                                                                        <  3:176094/148‑1 (MQ=255)
                                                               gCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGc                                                                                      >  2:115240/1‑149 (MQ=255)
                                                                ccATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGcc                                                                                     <  4:315582/149‑1 (MQ=255)
                                                                          gTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggc                                                                           <  8:271522/149‑1 (MQ=255)
                                                                             gCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcggc                                                                        <  1:115240/149‑1 (MQ=255)
                                                                              ccggtttCGGCGGGGCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcgg                                                                         >  8:253252/7‑147 (MQ=255)
                                                                              ccggtttCGGCGGGGCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcgg                                                                         <  7:253252/141‑1 (MQ=255)
                                                                              ccTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcggcg                                                                       >  1:151277/1‑149 (MQ=255)
                                                                               cTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAgcggcggcg                                                                       >  2:111631/1‑148 (MQ=255)
                                                                                 atatCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAg                                                                    >  5:198737/1‑149 (MQ=255)
                                                                                    tCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTg                                                                 >  6:85840/1‑149 (MQ=255)
                                                                                       gcggcCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTg                                                              >  8:2156/1‑149 (MQ=255)
                                                                                           ccccGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACGTCACCAGCCCGGGTGGCCAAGCAGCGGCGGCGCCGGTGCGGGAAg                                                          >  7:312713/1‑149 (MQ=255)
                                                                                            cccGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAgg                                                         >  3:99641/1‑149 (MQ=255)
                                                                                            cccGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCACCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAgg                                                         >  1:71073/1‑149 (MQ=255)
                                                                                             ccgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                                           >  1:187717/1‑146 (MQ=255)
                                                                                             ccgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                                           <  2:187717/146‑1 (MQ=255)
                                                                                             ccgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAgg                                                         >  7:248226/1‑148 (MQ=255)
                                                                                              cgccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGc                                                        >  6:345586/1‑148 (MQ=255)
                                                                                               gccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGc                                                        >  2:56085/1‑147 (MQ=255)
                                                                                                ccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGc                                                                >  3:187421/1‑138 (MQ=255)
                                                                                                ccgAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGc                                                                <  4:187421/138‑1 (MQ=255)
                                                                                                       cGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGACGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCt                                              >  1:76016/1‑149 (MQ=255)
                                                                                                         gggTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCg                                            >  4:229018/1‑149 (MQ=255)
                                                                                                         gggTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCg                                            <  5:246605/149‑1 (MQ=255)
                                                                                                          ggTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCgg                                           >  8:319091/1‑149 (MQ=255)
                                                                                                                ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCACGCGGCCCTCGGCGAtt‑t                                    >  4:33581/1‑149 (MQ=255)
                                                                                                                ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGc                                                    >  5:88803/1‑134 (MQ=255)
                                                                                                                ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGc                                                    <  6:88803/134‑1 (MQ=255)
                                                                                                                ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCgg                                           >  5:345732/1‑143 (MQ=255)
                                                                                                                ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCgg                                           <  6:345732/143‑1 (MQ=255)
                                                                                                                ccAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGAtt‑t                                    >  5:90398/1‑149 (MQ=255)
                                                                                                                            caccGGCTTCGGCCATGGCACGGCGGAAGCCGCTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                                           >  6:126644/4‑115 (MQ=37)
                                                                                                                            caccGGCTTCGGCCATGGCACGGCGGAAGCCGCTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGaa                                                           <  5:126644/112‑1 (MQ=37)
                                                                                                                             accGGCTTCGGCCATGGCACGGCGGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGa                       >  3:166814/3‑149 (MQ=255)
                                                                                                                                  cTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAg                                                          >  3:277587/1‑110 (MQ=255)
                                                                                                                                  cTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAg                                                          <  4:277587/110‑1 (MQ=255)
                                                                                                                                  cTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGa                  >  5:194455/1‑149 (MQ=255)
                                                                                                                                   ttCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCgg                             >  4:32929/1‑137 (MQ=255)
                                                                                                                                   ttCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCgg                             <  3:32929/137‑1 (MQ=255)
                                                                                                                                   ttCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGAt                 >  5:314577/1‑149 (MQ=255)
                                                                                                                                         cgTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCtt           <  6:314577/149‑1 (MQ=255)
                                                                                                                                         cgTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCtt           <  4:212858/149‑1 (MQ=255)
                                                                                                                                          gTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCttt          <  5:85840/149‑1 (MQ=255)
                                                                                                                                          gTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCttt          >  1:17847/1‑149 (MQ=255)
                                                                                                                                           tGCCATGGCCGAAGCCGGTGCGCCCGTGCCTGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCTTTg         <  6:271133/149‑1 (MQ=255)
                                                                                                                                             ccATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCTTTGgt       <  6:194455/149‑1 (MQ=255)
                                                                                                                                             ccATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCTTTGgt       <  2:71073/149‑1 (MQ=255)
                                                                                                                                             ccATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCTTTGgt       <  2:17847/149‑1 (MQ=255)
                                                                                                                                               aTGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCTTTGgtgt     >  6:13103/1‑149 (MQ=255)
                                                                                                                                               aTGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCTTTGgtgt     >  8:142827/1‑149 (MQ=255)
                                                                                                                                               aTGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCTTTGgtgt     >  1:325456/1‑149 (MQ=255)
                                                                                                                                                tGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCTTTGGtgtg    >  3:357119/1‑149 (MQ=255)
                                                                                                                                                   ccGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATTATTTGCCGGCAACGACATGATCGGCTTTGGTGtgct  <  1:56085/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ACCTGGTGCGCATTGACCACGAGCAGGGGGCGTACCTGGCCACCCGGCATCTGCTGGAGCTTGGCCATCGCGACGTCGCCTATATCGGCGGCCCCGCCGAAACCGGGGTTACCCAGCTGCGCCTGAGCGGCTTCCGCCGTGCCATGGCCGAAGCCGGTGCGCCCGTGCCGGGCAGCCGTGTACTGCACTGCGACTTCACCAGCCCGGGTGGCCATGCAGCGGCGGCGCAGGTGCTGGAAGGCAAGCGGCCCTCGGCGATT‑TTTGCCGGCAACGACATGATCGGCTTTGGTGTGCT  >  NC_002947/2804648‑2804942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑