Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,868,491 C→G 5.7% intergenic (+30/‑901) PP_2520 → / → PP_2522 hypothetical protein/transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,868,4910CG 5.7% 218.9 / 3.7 70intergenic (+30/‑901)PP_2520/PP_2522hypothetical protein/transposase
Reads supporting (aligned to +/- strand):  ref base C (34/32);  new base G (2/2);  total (36/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.83e-01

ATGTCGCGTCAAGCTGTGCCGCTCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACATTCCCGCGCTGGCTGACG  >  NC_002947/2868345‑2868630
                                                                                                                                                  |                                                                                                                                           
aTGTCGCGTCAAGCTGTGCCGCTCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTc                                                                                                                                             >  2:369795/1‑147 (MQ=255)
  gTCGCGTCAAGCTGTGCCGCTCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCaag                                                                                                                                          >  2:75676/1‑148 (MQ=255)
   tCGCGTCAAGCTGTGCCGCTCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCaagaa                                                                                                                                        <  8:40863/149‑1 (MQ=255)
     gcgTCAAGCTGTGCCGCTCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAagaaga                                                                                                                                      <  7:152054/149‑1 (MQ=255)
     gcgTCAAGCTGTGCCGATCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGATTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCATCTCAGTCTTCGTCAataaga                                                                                                                                      >  6:147021/1‑149 (MQ=255)
          aaGCTGTGCCGCTCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGcc                                                                                                                                 >  7:168511/1‑149 (MQ=255)
          aaGCTGTGCCGCTCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAAAGTGTGATCTCTCCCGCTCAGTCTTCTTGACGAAGACTga                                                                                                                                  <  5:306897/148‑2 (MQ=255)
           aGCTGTGCCGCTCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCTTGACGAAGACTgagc                                                                                                                                <  5:289197/149‑4 (MQ=255)
             cTGTGCCGCTCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCt                                                                                                                              >  5:215585/1‑149 (MQ=255)
                         gcagcaAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTg                                                                                                                  >  3:75536/1‑149 (MQ=255)
                           agcaAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTggg                                                                                                                <  8:168511/149‑1 (MQ=255)
                               aGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGAtt                                                                                                            <  5:50041/149‑1 (MQ=255)
                                  tGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAagaaga                                                                                                                                      <  6:198438/120‑1 (MQ=255)
                                  tGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAagaaga                                                                                                                                      >  5:198438/1‑120 (MQ=255)
                                  tGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTggg                                                                                                                <  5:64892/142‑1 (MQ=255)
                                  tGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTggg                                                                                                                >  6:64892/1‑142 (MQ=255)
                                    ttGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATAc                                                                                                       <  3:126179/149‑1 (MQ=255)
                                     tGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCACTCGCCGTCGTCTGGTGATTGATACa                                                                                                      >  5:70596/1‑149 (MQ=255)
                                       gCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAgg                                                                                                    >  4:209750/1‑149 (MQ=255)
                                       gCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAg                                                                                                     <  7:305081/148‑1 (MQ=255)
                                          tGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGcc                                                                                                 >  1:109256/1‑149 (MQ=255)
                                           gTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATAc                                                                                                       <  1:78181/142‑1 (MQ=255)
                                           gTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATAc                                                                                                       >  2:78181/1‑142 (MQ=255)
                                            tAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGcc                                                                                                 <  1:162741/147‑1 (MQ=255)
                                            tAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCtt                                                                                               >  1:274088/1‑149 (MQ=255)
                                             aaaaGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTc                                                                                              >  7:71719/1‑149 (MQ=255)
                                                aGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCAt                                                                                           >  1:276235/1‑149 (MQ=255)
                                                aGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCAt                                                                                           <  3:209750/149‑1 (MQ=255)
                                                aGACCTCATGCGATGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATTCAGGGCCTTCCAt                                                                                           <  5:147021/149‑1 (MQ=255)
                                                    cTCATGCGCTGCTGGCGTTGTCAGGCGTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCa                                                                                        >  1:10195/1‑148 (MQ=255)
                                                          cgcTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCACTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTg                                                                                 >  6:315320/1‑149 (MQ=255)
                                                            ctgctgGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGGCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGtt                                                                               <  1:75676/149‑1 (MQ=255)
                                                             tgctgGCGTTGGCAGGCTGCCTGGTGCTGATGCCGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGGCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTg                                                                              <  6:215585/149‑1 (MQ=255)
                                                                 ggCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAAc                                                                           >  6:378007/1‑148 (MQ=255)
                                                                  gCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAAc                                                                           >  2:249893/1‑147 (MQ=255)
                                                                    gTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGTGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAAc                                                                           >  7:170566/1‑145 (MQ=255)
                                                                    gTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAAc                                                                           >  6:161855/1‑145 (MQ=255)
                                                                    gTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACgaga                                                                       <  1:369795/149‑1 (MQ=255)
                                                                       gTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATtc                                                                    >  4:167782/1‑149 (MQ=255)
                                                                        tCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATtc                                                                    >  2:336298/1‑148 (MQ=255)
                                                                        tCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATtc                                                                    <  1:336298/148‑1 (MQ=255)
                                                                          aGGCTTCCTGGTGATGATGACGCTTATGGCGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATtctc                                                                  <  2:106583/148‑1 (MQ=255)
                                                                            gCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCTCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAAAGCTGTTGAACGAGATTCTCgaa                                                               >  4:128/1‑149 (MQ=255)
                                                                            gCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCgaa                                                               >  3:135858/1‑149 (MQ=255)
                                                                            gCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCg                                                                 >  3:130383/1‑147 (MQ=255)
                                                                             cTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAGCGAGATTCTCgaag                                                              <  5:312356/149‑1 (MQ=255)
                                                                              ttCCTGGTGATGATGACGCTTTTGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGATCAGTCTTCGTCATGAAGAGTGCCCCTCGCCGTCGTCTGTGGATTGATACAGGGCCTTCCATGCAAACGGTGTTGAACGAGATTCTCgaaga                                                             <  5:385817/149‑1 (MQ=255)
                                                                                ccTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGTGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCgaagaa                                                            <  4:75536/148‑1 (MQ=255)
                                                                                        tgatgaCGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTccc                                                   >  1:105646/1‑149 (MQ=255)
                                                                                        tgatgaCGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTccc                                                   <  2:10195/149‑1 (MQ=255)
                                                                                             aCGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGAt                                              <  8:71719/149‑1 (MQ=255)
                                                                                              cGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATc                                             >  7:127157/1‑149 (MQ=255)
                                                                                                 ttATGGTGATTAGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGGCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCgg                                           >  4:196497/1‑148 (MQ=255)
                                                                                                    tggagctttGTTTCTCAATGTCTGATCTCTCCCGCTCATTCTACGTCAACCAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACTGGGCCTTCCATGCAATCTCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAg                                       <  8:89973/143‑1 (MQ=255)
                                                                                                    tGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAg                                       <  5:161855/149‑1 (MQ=255)
                                                                                                              tttCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCg                             >  7:85524/1‑149 (MQ=255)
                                                                                                               ttCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCAATCCATGCAAACGCTGATGAACCAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCg                             >  3:14243/1‑148 (MQ=255)
                                                                                                                 cTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTctct                           <  6:70596/148‑1 (MQ=255)
                                                                                                                 cTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCt                           >  1:376117/1‑148 (MQ=255)
                                                                                                                  tGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGa                         >  6:281486/1‑149 (MQ=255)
                                                                                                                    aaTGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGa                         <  5:378007/147‑1 (MQ=255)
                                                                                                                    aaTGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGAc                        <  3:167782/148‑1 (MQ=255)
                                                                                                                          tgATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACAtt                  <  1:249893/148‑1 (MQ=255)
                                                                                                                            aTCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACATTccc               <  8:85524/149‑1 (MQ=255)
                                                                                                                              ctctcCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACATTccccc             >  7:296485/1‑147 (MQ=255)
                                                                                                                                 tcCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACATTCCcgc             <  2:274088/146‑1 (MQ=255)
                                                                                                                                  cccGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACATTCCCGCgctg         >  1:359625/1‑149 (MQ=255)
                                                                                                                                   ccGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACATTCCCGCgct          <  2:105646/147‑1 (MQ=255)
                                                                                                                                     gCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACATTCCCGCgctggct      <  8:127157/149‑1 (MQ=255)
                                                                                                                                        cAGTCTTCTTGACGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACATTCCCGCGCTGGCTGAc   >  7:60977/1‑149 (MQ=255)
                                                                                                                                         aGTCTTCTTGACGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACATTCCCGCGCTGGCTGACg  >  4:78028/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
ATGTCGCGTCAAGCTGTGCCGCTCGGCAGCAAGCTGTTGGCCTGTAAAAGACCTCATGCGCTGCTGGCGTTGTCAGGCTTCCTGGTGATGATGACGCTTATGGTGATTTGTTTCTGAATGTGTGATCTCTCCCGCTCAGTCTTCGTCAAGAAGACTGCCCCTCGCCGTCGTCTGGGGATTGATACAGGGCCTTCCATGCAAACGCTGTTGAACGAGATTCTCGAAGAAGTACGTCCCTTGATCGGCAAGGGCAAAGTCGCTGACTACATTCCCGCGCTGGCTGACG  >  NC_002947/2868345‑2868630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑