Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,885,730 C→A 10.7% R50L (CGC→CTC)  PP_2541 ← transcriptional factor‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,885,7300CA10.7% 100.6 / 2.6 36R50L (CGC→CTC) PP_2541transcriptional factor‑like protein
Reads supporting (aligned to +/- strand):  ref base C (19/12);  new base A (2/2);  total (22/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGTGTGTCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGCGGGTCGGGTGGCTG  >  NC_002947/2885588‑2885873
                                                                                                                                              |                                                                                                                                               
gacgTGTGTCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCGCGACCTGGTCGAGCTCgc                                                                                                                                           <  2:265929/149‑2 (MQ=255)
           gTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTg                                                                                                                                <  1:220587/149‑1 (MQ=255)
                  aaGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGTCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCtggtg                                                                                                                          <  4:16840/148‑1 (MQ=255)
                    gTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTa                                                                                                                       <  6:383154/149‑1 (MQ=255)
                                     tgctgcGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAg                                                                                                      >  3:169037/1‑149 (MQ=255)
                                                gCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGCCGAGCTCGACCAGGTCGCGCACGACGGTGCGTTTGCTGGTGGTATGGCCCCCGGTTGTCAGGGCAGCCGGc                                                                                            >  2:344826/1‑148 (MQ=255)
                                                 cccAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCaa                                                                                          >  4:224814/1‑149 (MQ=255)
                                                    aGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTg                                                                                       >  7:211877/1‑149 (MQ=255)
                                                      gCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTggg                                                                                     >  1:317684/1‑149 (MQ=255)
                                                        tCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTTAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTg                                                                                   >  5:358423/1‑149 (MQ=255)
                                                          aaGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTg                                                                                   >  6:69045/1‑147 (MQ=255)
                                                                    gCCAGTACCAGACATAAGGCATGCCTTTACTGTTGAACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGCCGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTccc                                                                       >  6:335429/1‑149 (MQ=255)
                                                                     ccAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCg                                                                      >  5:47827/1‑149 (MQ=255)
                                                                      cAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTg                                                                                             >  8:123571/1‑125 (MQ=255)
                                                                      cAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTg                                                                                             <  7:123571/125‑1 (MQ=255)
                                                                        gTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCTAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTTGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGt                                                                   <  7:259962/149‑1 (MQ=255)
                                                                                  tAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCggg                                                         <  5:181105/149‑1 (MQ=255)
                                                                                               tACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGa                                              <  3:224814/147‑1 (MQ=255)
                                                                                               tACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGa                                              <  4:169037/147‑1 (MQ=255)
                                                                                                    ttGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTg                                       >  8:71168/1‑149 (MQ=255)
                                                                                                    ttGCACTGAAGCGGGAACAGTGCGGCGAGCTAGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTg                                       >  2:360446/1‑149 (MQ=255)
                                                                                                       cACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCa                                    >  2:185212/1‑149 (MQ=255)
                                                                                                        aCTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCAc                                   <  2:317684/149‑1 (MQ=255)
                                                                                                              gCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGTGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTggcgg                             >  5:32642/1‑149 (MQ=255)
                                                                                                              gCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTggcgg                             >  8:110476/1‑149 (MQ=255)
                                                                                                                       gTGCGGCGAGCTCGACCAGGTCGCGCTCGACAGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGgtg                    >  1:227683/1‑149 (MQ=255)
                                                                                                                        accgtcgAGCGCGCCCTGGTCGAGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCGACTGGCGGGCGATGgtgt                   >  2:266134/6‑149 (MQ=255)
                                                                                                                           gtcgAGCGCGACCTGGTCGAGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGc                >  4:161377/3‑149 (MQ=255)
                                                                                                                             cgAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGcgg              <  5:69045/149‑1 (MQ=255)
                                                                                                                              gAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGcggg             >  8:139686/1‑149 (MQ=255)
                                                                                                                              gAGCGCGACCTGGTCGTGCTCGACAGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGcggg             >  2:331386/1‑149 (MQ=255)
                                                                                                                                 cTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGcgggtcg          >  4:73858/1‑149 (MQ=255)
                                                                                                                                   cGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGcgggtcggg        >  2:80127/1‑149 (MQ=255)
                                                                                                                                         ggTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGc                                      <  4:180061/113‑1 (MQ=255)
                                                                                                                                         ggTCGCGCTCGACGGGGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCTGCCGTGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGCGGGTCGGGTGGCTg  <  5:335429/149‑1 (MQ=255)
                                                                                                                                          gTCGAGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGCGGGTCGGGTGGCTg  <  3:161377/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
GACGTGTGTCGGTGGGGTAAGTGCGTCGGGTTGCAGCTGCTGCGCTTCGCCCAGGCTCAAGCCCGGTTGCCAGTACCAGCCATAAGGCATGCCTTTACTGTTGCACTGAAGCGGGAACAGTGCGGCGAGCTCGACCAGGTCGCGCTCGACGGTGCGTTTGCTGGTGGTATGGCCCACGGTTGTCAGGGCAGCCTGCAACTGGGTGGAGCTCATTCCCGGGTGGCGGCCGGGCAGCAACTTGAGCAGTTGCCACTGGCGGGCGATGGTGTGGCGGGTCGGGTGGCTG  >  NC_002947/2885588‑2885873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑