Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,886,149	A→T	7.7%	M282K (ATG→AAG)	PP_5536 ←	conserved protein of unknown function with RHS repeat‑associated core domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,886,149	0	A	T	7.7%	177.8 / 3.2	62	M282K (ATG→AAG)	PP_5536	conserved protein of unknown function with RHS repeat‑associated core domain
Reads supporting (aligned to +/- strand):  ref base A (29/28);  new base T (3/2);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGGCCTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTGTTCGG  >  NC_002947/2886003‑2886295                                                                                                                                                    |                                                                                                                                                   gAGGCCTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATa                                                                                                                                                  >  4:325947/1‑149 (MQ=255)  aGGCATTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAg                                                                                                                                                 <  8:354576/149‑1 (MQ=255)     ccTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGtt                                                                                                                                              <  1:242433/149‑1 (MQ=255)     ccTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGtt                                                                                                                                              <  2:66607/149‑1 (MQ=255)      cTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGtt                                                                                                                                              >  6:78803/1‑148 (MQ=255)       tttGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTg                                                                                                                                             >  1:267146/1‑148 (MQ=255)       tttGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTg                                                                                                                                             >  2:360512/1‑148 (MQ=255)           tgGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAAc                                                                                                                                         <  6:136836/148‑1 (MQ=255)             ggAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCt                                                                                                                                       <  8:183486/148‑1 (MQ=255)                    gCCGAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAggg                                                                                                                                <  5:3918/148‑1 (MQ=255)                    gCCCAGCGCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCTACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGGTGAAACCTGACAggg                                                                                                                                <  8:268261/148‑1 (MQ=255)                    gCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAggg                                                                                                                                <  7:21145/148‑1 (MQ=255)                      ccAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGgtg                                                                                                                             >  5:193865/1‑149 (MQ=255)                            ccTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAAtt                                                                                                                        >  4:293619/1‑148 (MQ=255)                            ccTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTg                                                                                                                       <  4:150328/149‑1 (MQ=255)                                tGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGaa                                                                                                                     <  1:139077/147‑1 (MQ=255)                                      cgcCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTg                                                                                                              <  3:325947/148‑1 (MQ=255)                                             gcgcAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTtata                                                                                                      <  3:293619/149‑1 (MQ=255)                                                           gCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCa                                                                                        <  2:267146/149‑1 (MQ=255)                                                           gCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCa                                                                                        <  6:119833/149‑1 (MQ=255)                                                                   gCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAgattttg                                                                                <  5:78803/149‑1 (MQ=255)                                                                   gCGGGCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAgattttg                                                                                <  1:341671/149‑1 (MQ=255)                                                                    cGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAgattttga                                                                               >  3:137012/1‑149 (MQ=255)                                                                      gTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTtata                                                                                                      >  7:58984/1‑124 (MQ=255)                                                                      gTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTtata                                                                                                      <  8:58984/124‑1 (MQ=255)                                                                          gCGTACCCGGACAAAAAGTGCTTCCTTGGAGATGTATTACGTGCGACTCCGAACGCTTTCGG‑CCTGTTCAACCTTATGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTtg                                                                         <  3:208217/149‑1 (MQ=255)                                                                                  ggACAAACAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGACCGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTAttgt                                                                 <  8:50832/149‑1 (MQ=255)                                                                                    aCAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGGACCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTAttgtt                                                                >  1:280422/1‑149 (MQ=255)                                                                                      aaaaaGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTAttgtttgt                                                             >  8:302267/1‑149 (MQ=255)                                                                                        aaaGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCt                                                           >  5:73742/1‑149 (MQ=255)                                                                                        aaaGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCt                                                           <  2:125782/149‑1 (MQ=255)                                                                                        aaaGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCATGCGG‑CCTGTTCAACCATAGGTTGAAACCTGAAAGGGTTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTCTATTGTTTGTCt                                                           >  7:333902/1‑149 (MQ=255)                                                                                                gCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTgg                                                   >  8:277179/1‑149 (MQ=255)                                                                                                 ccTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGc                                                  >  4:269756/1‑149 (MQ=255)                                                                                                   ttGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCtt                                                >  8:227917/1‑149 (MQ=255)                                                                                                    tGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTg                                               >  7:73404/1‑149 (MQ=255)                                                                                                     gCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACa                                                                                                                                   >  8:242223/1‑64 (MQ=255)                                                                                                       aGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTtgg                                             >  3:203601/1‑148 (MQ=255)                                                                                                           gTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCg                                        >  7:167824/1‑149 (MQ=255)                                                                                                            tATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTAttgtttgt                                                             <  5:257338/127‑1 (MQ=255)                                                                                                            tATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTAttgtttgt                                                             >  6:257338/1‑127 (MQ=255)                                                                                                            tATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGt                                       >  8:81993/1‑149 (MQ=255)                                                                                                             aTTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTAtt                                                                   <  1:375867/120‑1 (MQ=255)                                                                                                             aTTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTAtt                                                                   >  2:375867/1‑120 (MQ=255)                                                                                                              ttACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTtat                                                                                                       >  1:20600/1‑83 (MQ=255)                                                                                                              ttACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTtat                                                                                                       <  2:20600/83‑1 (MQ=255)                                                                                                              ttACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGGCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGc                                     <  2:112024/149‑1 (MQ=255)                                                                                                                   tGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACg                                >  8:240470/1‑149 (MQ=255)                                                                                                                         tCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACATGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTGTTGTTGTGGCTTGGGTATCGTGATGACGGTTCCg                          >  7:234580/1‑149 (MQ=255)                                                                                                                                      gg‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTa             <  6:193865/149‑1 (MQ=255)                                                                                                                                          cTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGa          <  7:277179/149‑1 (MQ=255)                                                                                                                                           tGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGAt         >  5:275942/1‑149 (MQ=255)                                                                                                                                           gtttCAACCTATGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGAt         >  7:122527/3‑149 (MQ=255)                                                                                                                                           gtttCAACCTATGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGAt         >  5:94985/3‑149 (MQ=255)                                                                                                                                           gtttCAACCTATGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGAt         >  5:264262/3‑149 (MQ=255)                                                                                                                                             ttCAACCTATGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGAtt        <  8:122527/148‑1 (MQ=255)                                                                                                                                             ttCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGAtt        <  3:269756/148‑1 (MQ=255)                                                                                                                                               cAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTGtt     >  8:13984/1‑149 (MQ=255)                                                                                                                                               cAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTGt      <  8:167824/148‑1 (MQ=255)                                                                                                                                                aaCCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTGTTc    >  5:77077/1‑149 (MQ=255)                                                                                                                                                aaCCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTGTTc    <  7:227917/149‑1 (MQ=255)                                                                                                                                                  ccATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGCTATTAAAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGATTGGGTATCGTGCTGACGGTTCAGCTATACGCTTTTAAGATTGTTAgg  >  5:274701/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                   GAGGCCTTTGTGGGAGATCGCCCAGCCCCTTTGACTGCGCCGTCGCGCAGAGAACTCAGCGTGCAAGCGGTCAGCGTACCCGGACAAAAAGGGCTGCCTTGCAGATGTATTACGTGCGACTCCGAACGCTTGCGG‑CCTGTTCAACCATAGGTTGAAACCTGACAGGGGTGTAATTGAAGTCTTTGGTTTTATAGATGTTTTTTTGCAAGATTTTGATTTTTGTGTATTGTTTGTCTTGTTGTGGCTTGGGTATCGTGCTGACGGTTCCGCTATACGCTTTTACGATTGTTCGG  >  NC_002947/2886003‑2886295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑