Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,915,588 G→A 6.0% A1536A (GCG→GCA)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,915,5880GA 6.0% 198.1 / 2.0 66A1536A (GCG→GCA) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (31/31);  new base A (2/2);  total (33/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.22e-01

TGCCAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCGGTTTCTTCGGCCCAGGTTCC  >  NC_002947/2915441‑2915735
                                                                                                                                                   |                                                                                                                                                   
tGCCAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCgg                                                                                                                                                    <  2:92205/149‑1 (MQ=255)
  ccAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGt                                                                                                                                                   <  3:361889/148‑1 (MQ=255)
   cAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTgg                                                                                                                                                 <  6:26887/149‑1 (MQ=255)
   cAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTg                                                                                                                                                  >  8:5919/1‑148 (MQ=255)
   cAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTg                                                                                                                                                  >  4:186448/1‑148 (MQ=255)
   cAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTg                                                                                                                                                  >  7:208867/1‑148 (MQ=255)
    aGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGa                                                                                                                                                >  6:120863/1‑149 (MQ=255)
     gTGACGGCGGTGCCGGCCTGCAGATGGTGCGCTCCGACCTTAACTTCATCCTCGCGCTAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGAt                                                                                                                                               <  6:87407/149‑1 (MQ=255)
     gTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGAt                                                                                                                                               <  3:179120/149‑1 (MQ=255)
        aCGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGAt                                                                                                                                               >  1:23458/1‑146 (MQ=255)
        aCGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGAt                                                                                                                                               <  2:23458/146‑1 (MQ=255)
        aCGGCGGTGCCGGCCTACACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGc                                                                                                                                            >  5:31866/1‑149 (MQ=255)
          ggcggTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCgg                                                                                                                                                    >  5:155335/1‑139 (MQ=255)
          ggcggTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCgg                                                                                                                                                    <  6:155335/139‑1 (MQ=255)
             ggTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGaa                                                                                                                                       <  4:362898/149‑1 (MQ=255)
              gTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGaac                                                                                                                                      <  8:208867/149‑1 (MQ=255)
              gTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGaa                                                                                                                                       >  8:48899/1‑148 (MQ=255)
                gCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGaacaa                                                                                                                                    <  2:104612/149‑1 (MQ=255)
                gCCGGCCTACACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGaacaa                                                                                                                                    <  6:31866/149‑1 (MQ=255)
                         cacaTGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCtga                                                                                                                            <  1:234551/148‑1 (MQ=255)
                                   gcTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAAc                                                                                                                 <  2:338735/149‑1 (MQ=255)
                                        gACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCGGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGcaa                                                                                                            <  5:319918/149‑1 (MQ=255)
                                         aCCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAAc                                                                                                                       >  2:283930/1‑137 (MQ=255)
                                         aCCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAAc                                                                                                                       <  1:283930/137‑1 (MQ=255)
                                             gAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGcaacaac                                                                                                        >  8:33290/1‑148 (MQ=255)
                                             gAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGcaacaac                                                                                                        >  6:75384/1‑148 (MQ=255)
                                                 ttCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGaacaa                                                                                                                                    <  1:324559/116‑1 (MQ=255)
                                                 ttCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGaacaa                                                                                                                                    >  2:324559/1‑116 (MQ=255)
                                                 ttCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAacac                                                                                                   >  4:91855/1‑149 (MQ=255)
                                                      ccTCGCTCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCGGATCCCCTACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAAt                                                                                              <  8:270352/149‑1 (MQ=255)
                                                        tCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCAGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATAc                                                                                            >  3:150072/1‑149 (MQ=255)
                                                        tCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCAGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATAc                                                                                            >  3:144563/1‑149 (MQ=255)
                                                              aaaTCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTgcc                                                                                      <  5:120863/149‑1 (MQ=255)
                                                                aTCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTgccgc                                                                                    >  2:103805/1‑149 (MQ=255)
                                                                    aGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGa                                                                                 >  3:166345/1‑148 (MQ=255)
                                                                    aGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGa                                                                                 >  7:106120/1‑148 (MQ=255)
                                                                     gATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGAc                                                                                >  4:352124/1‑148 (MQ=255)
                                                                       ttGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAAc                                                                             >  6:351678/1‑149 (MQ=255)
                                                                           cGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCt                                                                         >  2:20006/1‑149 (MQ=255)
                                                                             aGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTc                                                                       <  7:48899/149‑1 (MQ=255)
                                                                                 cGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCcgc                                                                   >  5:95934/1‑149 (MQ=255)
                                                                                   aTGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCcgcgc                                                                 <  1:103805/149‑1 (MQ=255)
                                                                                       cgccgTGCAGGACATCCTCTAGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATTGCTCGAACAACAACCTGATGAAGCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTga                                                             <  6:305154/149‑1 (MQ=255)
                                                                                        gccgGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTgac                                                            >  4:366518/1‑149 (MQ=255)
                                                                                             gCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGCGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTga                                                             <  7:1757/143‑1 (MQ=255)
                                                                                             gCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGCGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTga                                                             >  8:1757/1‑143 (MQ=255)
                                                                                              cAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGAccc                                                      <  2:267010/149‑1 (MQ=255)
                                                                                               aGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCg                                                     >  7:261430/1‑149 (MQ=255)
                                                                                                  aCATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGCCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCCGTa                                                  >  1:55717/1‑149 (MQ=255)
                                                                                                     tCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTc                                                                          >  1:136185/1‑122 (MQ=255)
                                                                                                     tCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTc                                                                          <  2:136185/122‑1 (MQ=255)
                                                                                                      ccTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTc                                               >  6:379030/1‑148 (MQ=255)
                                                                                                            gCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCg                                         <  6:321650/148‑1 (MQ=255)
                                                                                                            gCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCAGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCTGACAACGTCTTCCCGCGCGTGACCGACCAGGTATTCAAccc                                          <  4:144563/147‑1 (MQ=255)
                                                                                                            gCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCAGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCTGACAACGTCTTCCCGCGCGTGACCGACCAGGTATTCAAccc                                          <  4:150072/147‑1 (MQ=255)
                                                                                                                 tCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGaa                                   <  3:366518/149‑1 (MQ=255)
                                                                                                                  ccccAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAg                                  >  1:10132/1‑149 (MQ=255)
                                                                                                                      aaCGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTgc                              >  5:208047/1‑149 (MQ=255)
                                                                                                                      aaCGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTgc                              >  4:215528/1‑149 (MQ=255)
                                                                                                                      aaCGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTgc                              <  5:75384/149‑1 (MQ=255)
                                                                                                                        cGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTgcgc                            <  6:95934/149‑1 (MQ=255)
                                                                                                                            cGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGc                        <  5:379030/149‑1 (MQ=255)
                                                                                                                              tGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCg                      <  3:352124/149‑1 (MQ=255)
                                                                                                                                cAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCGGt                    <  7:33290/149‑1 (MQ=255)
                                                                                                                                 aGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCGGtt                   >  5:32875/1‑149 (MQ=255)
                                                                                                                                   ccATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCGGTttc                 >  1:366202/1‑149 (MQ=255)
                                                                                                                                     aTGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACACCAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCGGTttctt               >  8:170348/1‑149 (MQ=255)
                                                                                                                                        ggCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCGGTTTCTTCg             <  7:170348/148‑1 (MQ=255)
                                                                                                                                           cTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCGGTTTCTTCGGccc         <  7:5919/149‑1 (MQ=255)
                                                                                                                                              cgtgcgGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGACGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCGGTTTCTTCGGCCCAgg      >  1:5588/1‑149 (MQ=255)
                                                                                                                                               gtgcgGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTAt                                                 >  3:347479/1‑105 (MQ=255)
                                                                                                                                               gtgcgGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTAt                                                 <  4:347479/105‑1 (MQ=255)
                                                                                                                                                  cgGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCGGTTTCTTCGGCCCAGGTTcc  >  2:361427/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGCCAGTGACGGCGGTGCCGGCCTGCACATGGTGCGCTCCGACCTGAACTTCATCCTCGCGCAAATCAAGATTGCCGAGGCCGATGCCGCCGGGCAGGACATCCTCTCGCTGATCCCCAACGTCCGTGCAGCCATGGGCCTGCGTGCGGTGGATGGCTCGAACAACAACCTGATGAACCTGAACGGCAACAACAACACCCAATACGGTGCCGCCGACAACGTCTTCCCGCGCGTGACCGACCCGGTATTCAACCCGGCCGAAGGTGCGCCAGCCGGTTTCTTCGGCCCAGGTTCC  >  NC_002947/2915441‑2915735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑