Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,921,460 G→A 100% G3494R (GGG→AGG)  PP_2561 → hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,921,4600GA100.0% 95.2 / NA 29G3494R (GGG→AGG) PP_2561hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/10);  total (19/10)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC  >  NC_002947/2921316‑2921600
                                                                                                                                                |                                                                                                                                             
acGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagggg                                                                                                                                           <  4:172019/149‑1 (MQ=255)
                   gTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTcat                                                                                                                        >  4:19850/1‑146 (MQ=255)
                      aCGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTGCCTCAAc                                                                                                                    >  8:253009/1‑149 (MQ=255)
                        gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACgt                                                                                                                  >  4:64347/1‑148 (MQ=255)
                        gACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACgg                                                                                                                  >  4:256061/1‑149 (MQ=255)
                           aaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGc                                                                                                               >  5:164790/1‑149 (MQ=255)
                             ccTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCgg                                                                                                             >  3:109955/1‑149 (MQ=255)
                                 cccGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCCAACGACTTCCTCAACGGTGCGGCAg                                                                                                          <  1:193347/147‑1 (MQ=255)
                                   cGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTa                                                                                                       <  3:256061/149‑1 (MQ=255)
                                        tcgcTGGCGAGGACAGCCTGGATGGCGGCCACGGCAATGACACCCTGCAGGGCGGCGATGGTACCGACACATTGGTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGAt                                                                                                  <  2:376367/148‑1 (MQ=255)
                                                gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACAAATTGTTAGGCGACGTGGGTAACGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTTCGGCATGTAACGATAAggtggt                                                                                          >  1:285020/1‑149 (MQ=255)
                                                     cAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggtg                                                                                     <  4:109955/149‑1 (MQ=255)
                                                     cAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATAGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTggtgggtg                                                                                     >  5:194138/1‑149 (MQ=255)
                                                                        ggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTc                                                                                                                          <  2:263168/93‑1 (MQ=255)
                                                                        ggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTc                                                                                                                          >  1:263168/1‑93 (MQ=255)
                                                                         gcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACAcc                                                                    >  4:39722/1‑146 (MQ=255)
                                                                           aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGAt                                                               >  3:320345/1‑149 (MQ=255)
                                                                                 acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtg                                                                                            <  8:32686/114‑1 (MQ=255)
                                                                                 acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACggtg                                                                                            >  7:32686/1‑114 (MQ=255)
                                                                                    cTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGa                                                      >  4:175237/1‑149 (MQ=255)
                                                                                    cTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGa                                                      >  1:168243/1‑149 (MQ=255)
                                                                                        aGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATgg                                                   >  5:52945/1‑148 (MQ=255)
                                                                                           gcggcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAAc                                               <  2:168243/149‑1 (MQ=255)
                                                                                              gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGTCGGGTAACGACACCATGATCGCGACCGATGGAAACGAt                                            <  6:164790/149‑1 (MQ=255)
                                                                                              gcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGAt                                            <  6:52945/149‑1 (MQ=255)
                                                                                                         gACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGtt                                 >  3:178531/1‑149 (MQ=255)
                                                                                                                                       acCATGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat   <  3:39722/149‑1 (MQ=255)
                                                                                                                                         ccTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc  >  7:18017/1‑148 (MQ=255)
                                                                                                                                         ccTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc  >  2:314076/1‑148 (MQ=255)
                                                                                                                                         ccTGATCAGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCAGatcatc  >  1:358939/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                             
ACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGG‑CAACGACTTCCTCAACGGTGCGGCAGGTAACGATACGGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATC  >  NC_002947/2921316‑2921600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑