Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,944,263 T→C 8.1% I231T (ATC→ACC)  PP_2577 → paraquat‑inducible protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,944,2630TC 8.1% 139.8 / 2.4 49I231T (ATC→ACC) PP_2577paraquat‑inducible protein B
Reads supporting (aligned to +/- strand):  ref base T (24/21);  new base C (2/2);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

GATCTACTACCGCAAGATCCCGGTGGGTGAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAGGCCCGCTTCCAGCTATTC  >  NC_002947/2944117‑2944405
                                                                                                                                                  |                                                                                                                                              
gATCTACTACCGCAAGATCCCGGTGGGTGAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATc                                                                                                                                               >  5:162634/1‑148 (MQ=255)
    tactacCGCAAGATCCCGGTGGGTGAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACAt                                                                                                                                          >  8:82703/1‑149 (MQ=255)
     actacCGCAAGATCCCGGTGGGTGAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCATTGGCACCGatctg                                                                                                                                         >  8:242440/1‑145 (MQ=255)
      ctacCGCAAGATCCCGGTGGGTGAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGa                                                                                                                                             <  2:158933/144‑1 (MQ=255)
      ctacCGCAAGATCCCGGTGGGTGAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGc                                                                                                                                        <  2:126473/149‑1 (MQ=255)
      ctacCGCAAGATCCCGGTGGGTGAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCGTCTGGAATGCCAGTGGCATCGACATg                                                                                                                                         >  6:319355/1‑148 (MQ=255)
         ccGCAAGATCCCGGTGGGTGAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGTTTCTGGAATGCCAGTGGCATCGACATg                                                                                                                                         >  3:307489/1‑145 (MQ=255)
                      gtgggtgAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCATTGGCACCGATCTGCATGTCGATGCCacttg                                                                                                                        <  7:242440/149‑5 (MQ=255)
                      gtgggtgAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCATTGGCACCGATCTGCATGTCGATGCCACTgg                                                                                                                        <  8:278752/149‑1 (MQ=38)
                       tgggtgAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGTTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGc                                                                                                                       <  4:307489/149‑1 (MQ=255)
                         ggtgAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCt                                                                                                                     <  1:301092/149‑1 (MQ=255)
                                    gtCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGCCACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATa                                                                                                          >  6:141526/1‑149 (MQ=255)
                                       gtttGCCCTCCTGTACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATAcc                                                                                                        <  5:10417/147‑1 (MQ=255)
                                        tttGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCg                                                                                                       >  4:140131/1‑148 (MQ=255)
                                        tttGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCg                                                                                                       >  2:201260/1‑148 (MQ=255)
                                           gCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATc                                                                                                   >  4:49796/1‑149 (MQ=255)
                                             ccTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGAACGGTGCCAATGGCCTGAAGGTCGATACCGAATCGc                                                                                                 >  7:297634/1‑149 (MQ=255)
                                                        aTGGCACGGGGGGAGAGCGTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGCTACCGAATCGCTGTCGTCGAtt                                                                                      <  6:69236/149‑1 (MQ=255)
                                                        aTGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGAGACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCTGATCGGTGCGAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGAtt                                                                                      <  6:74599/149‑1 (MQ=255)
                                                           gCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCtg                                                                                   >  5:33310/1‑149 (MQ=255)
                                                                 gggTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTgggc                                                                             >  7:243979/1‑149 (MQ=255)
                                                                        gaTTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTgg                                                                      <  7:275015/149‑1 (MQ=255)
                                                                           tGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCt                                                                   >  4:175198/1‑149 (MQ=255)
                                                                                  ttCGTGCAGTCGCCCTACGACACGTTCGTCACTGCTGACAAGCGCTTCTGGACTGCCAGTGGGATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTc                                                            <  7:149264/149‑1 (MQ=255)
                                                                                  ttCGTGCAGGCGCCCTACGACAAGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTc                                                            <  6:102689/149‑1 (MQ=255)
                                                                                      tGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGACTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGccc                                                        >  2:346274/1‑149 (MQ=255)
                                                                                             gcCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTc                                                            <  4:64810/138‑1 (MQ=255)
                                                                                             gcCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTc                                                            >  3:64810/1‑138 (MQ=255)
                                                                                                     acacGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTgtcgtc                                                                                          <  3:731/100‑1 (MQ=255)
                                                                                                     acacGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTgtcgtc                                                                                          >  4:731/1‑100 (MQ=255)
                                                                                                     acacGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCgcgc                                           >  7:171222/1‑147 (MQ=255)
                                                                                                         gTTCGTCACTGCTGCCACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCg                                     >  7:319147/1‑149 (MQ=255)
                                                                                                          ttCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGcc                                      >  7:266956/1‑147 (MQ=255)
                                                                                                          ttCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGa                                    <  6:33310/149‑1 (MQ=255)
                                                                                                                  tgctgACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCctgctg                            >  1:39431/1‑149 (MQ=255)
                                                                                                                    ctgACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATCCCGAAGCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGcc                          <  5:319355/149‑1 (MQ=255)
                                                                                                                    ctgACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCctgctg                            <  3:140131/147‑1 (MQ=255)
                                                                                                                           gcgcTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAgg                   <  3:175198/149‑1 (MQ=255)
                                                                                                                           gcgcTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAgg                   <  8:266956/149‑1 (MQ=255)
                                                                                                                              cTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAGGccc                <  7:82703/149‑1 (MQ=255)
                                                                                                                                    gAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGAc                       >  7:287642/1‑136 (MQ=255)
                                                                                                                                    gAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGAc                       <  8:287642/136‑1 (MQ=255)
                                                                                                                                     aaTGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCCGCTGCCGACCAGGCCCGCTTCCa         <  1:201260/149‑1 (MQ=255)
                                                                                                                                      aTGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAATGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAGGCCCGCTTCCAg        <  8:297634/149‑1 (MQ=255)
                                                                                                                                        gCCATTGGCACCGATCTGCATGTCGATGCCACTGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAGGCCCGCTTCCAGc       >  6:343681/1‑148 (MQ=38)
                                                                                                                                         ccAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGTTACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAGGCCCGCTTCCAGCTa     >  1:262548/1‑149 (MQ=255)
                                                                                                                                         ccAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAGGCCCGCTTCCAGCTa     >  5:46969/1‑149 (MQ=255)
                                                                                                                                           aGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAGGCCCGCTTCCAGCTAtt   >  7:94131/1‑149 (MQ=255)
                                                                                                                                            gTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGACTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAGGCCCGCTTCCAGCTATTc  >  2:346390/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
GATCTACTACCGCAAGATCCCGGTGGGTGAGGTGGTGTCCTTTGCCCTCCAGGACGATGGCAAGGGGGTAGAGATTGGCGTGTTCGTGCAGGCGCCCTACGACACGTTCGTCACTGCTGACACGCGCTTCTGGAATGCCAGTGGCATCGACATGCAGATCGGTGCCAATGGCCTGAAGGTCGATACCGAATCGCTGTCGTCGATTCTGGTGGGCGGGCTGGCCTTCGGCTCGCCCGATTTCGCCGCGCAAGCCGAGCCTGCTGCCGACCAGGCCCGCTTCCAGCTATTC  >  NC_002947/2944117‑2944405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑