Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,008,052 A→G 7.3% M348T (ATG→ACG)  PP_2628 ← ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,008,0520AG 7.3% 160.3 / 2.9 55M348T (ATG→ACG) PP_2628ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (26/25);  new base G (2/2);  total (28/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTACCAGCTCTGCAGCACCCGGCACATCCTGCACCTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGTTGACGATTTCGAACTGGCTCACCAGGCGCATCTGG  >  NC_002947/3007906‑3008192
                                                                                                                                                  |                                                                                                                                            
ttACCAGCTCTGCAGCACCCGGCACATCCTGCACCTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATg                                                                                                                                            <  6:171323/149‑1 (MQ=255)
        tctGCAGCACCCGGCACATCCTGCACCTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCGTGGCggcaat                                                                                                                                    >  4:368533/1‑145 (MQ=255)
          tGCAGCACCCGGCACATCCTGCACCTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTg                                                                                                                                  >  6:333058/1‑149 (MQ=255)
            cagcaCCCGGCACATCCTGCACCTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTgcc                                                                                                                                >  3:192082/1‑149 (MQ=255)
            cagcaCCCGGCACATCCTGCACCTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTgcc                                                                                                                                >  6:364872/1‑149 (MQ=255)
                aCCCGGCACATCCTGCACCTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCa                                                                                                                            <  2:10453/149‑1 (MQ=255)
                    ggCACATCCTGCACCTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCGTGGCGGCAATCACCGCCATGGc                                                                                                                        <  3:368533/149‑1 (MQ=255)
                     gCACATCCTGCACCTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCGTGGCGGCAATCACCGCCATGGcc                                                                                                                       <  1:155803/149‑1 (MQ=255)
                                 ccTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTg                                                                                                                                      >  4:322966/1‑122 (MQ=255)
                                 ccTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTg                                                                                                                                      <  3:322966/122‑1 (MQ=255)
                                  cTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCt                                                                                                          <  6:201252/149‑1 (MQ=255)
                                           gcgGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGt                                                                                                 >  7:267505/1‑149 (MQ=255)
                                             gtgTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAAGAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTgc                                                                                               <  5:355039/147‑1 (MQ=255)
                                                 gAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTgcca                                                                                             >  8:148247/1‑147 (MQ=255)
                                                    ggTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTgccacagc                                                                                         >  6:12181/1‑148 (MQ=255)
                                                      tGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTgccacagccac                                                                                      <  4:269490/149‑1 (MQ=255)
                                                         ccATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGt                                                                                   <  4:192082/149‑1 (MQ=255)
                                                          cATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTg                                                                                  >  1:225008/1‑149 (MQ=255)
                                                             aCCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCg                                                                                >  7:341788/1‑148 (MQ=255)
                                                              ccGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTa                                                                              <  1:297094/149‑1 (MQ=255)
                                                                 tCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGTCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGcc                                                                           >  6:67749/1‑149 (MQ=255)
                                                                    tGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCAcc                                                                        >  2:21577/1‑149 (MQ=255)
                                                                     gCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTa                                                                              >  7:345985/1‑142 (MQ=255)
                                                                     gCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTa                                                                              <  8:345985/142‑1 (MQ=255)
                                                                      cACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCACCCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCgg                                                                      >  1:29421/1‑149 (MQ=255)
                                                                      cACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCg                                                                       <  5:225460/148‑1 (MQ=255)
                                                                       aCGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCgg                                                                      >  8:116666/1‑148 (MQ=255)
                                                                         ggTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCAc                                                                   <  7:116666/149‑1 (MQ=255)
                                                                              cGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCa                                                                 >  6:269767/1‑146 (MQ=255)
                                                                               gATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCa                                                                 >  6:90574/1‑145 (MQ=255)
                                                                                 tGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATc                                                           <  5:364872/149‑1 (MQ=255)
                                                                                   ttttCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCaa                                                         <  8:267505/149‑1 (MQ=255)
                                                                                     ttCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAg                                                        <  5:269767/148‑1 (MQ=255)
                                                                                     ttCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAg                                                        <  2:225008/148‑1 (MQ=255)
                                                                                       cGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGcc                                                      >  3:305982/1‑148 (MQ=255)
                                                                                              cGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCccaccacca                                              <  7:148247/149‑1 (MQ=255)
                                                                                               gAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCaa                                             >  3:181888/1‑149 (MQ=255)
                                                                                                  gTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCa                                                                 <  2:249900/126‑1 (MQ=255)
                                                                                                  gTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCa                                                                 >  1:249900/1‑126 (MQ=255)
                                                                                                     aTTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCACCCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATAAAGCCAAACACCAACGCCTg                                       >  6:263483/1‑149 (MQ=255)
                                                                                                     aTTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTg                                       >  2:365764/1‑149 (MQ=255)
                                                                                                        tCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGt                                     <  4:181888/148‑1 (MQ=255)
                                                                                                         cccACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGtt                                    <  2:29421/148‑1 (MQ=255)
                                                                                                          ccACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGtgag                                  >  2:136306/1‑146 (MQ=255)
                                                                                                          ccACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGTTGa                                  <  2:9320/149‑1 (MQ=255)
                                                                                                            acaTGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGTTGAc                                 >  1:77368/1‑148 (MQ=255)
                                                                                                                  tCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGTTGACGATTTc                           >  4:207754/1‑148 (MQ=255)
                                                                                                                  tCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGTTGACGATTTc                           >  8:252691/1‑148 (MQ=255)
                                                                                                                         ctccATACCGTTGCAGCTCAAGGGCATGGCGGTGATTGCCGCCACGGCCCCGGGGCCCACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGTTGAAGCCCACCACCAACGCCTGGTTGACGATTTCGAACTgg                    <  8:341788/145‑1 (MQ=255)
                                                                                                                             aTACCGTTGACGCGCAAGGCCATGGCGGTGATTGTCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGTTGACGATTTCGAACTGGCTCAc               <  5:67749/149‑1 (MQ=255)
                                                                                                                               aCCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCAGCACCAAAGATCAAGCCCCCCACCAACGCCTGGTTGACGATTTCGAACTGGCTCGccc             >  4:91271/1‑148 (MQ=255)
                                                                                                                                 cGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGTTGACGATTTCGAACTGGCTCACCAgg           <  5:90574/149‑1 (MQ=255)
                                                                                                                                  gTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGTTGACGATTTCGAACTGGCTCACCAGgc          <  7:252691/149‑1 (MQ=255)
                                                                                                                                          gcAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGTTGACGATTTCGAACTGGCTCACCAGGCGCATCTgg  <  5:333058/149‑1 (MQ=255)
                                                                                                                                          ccggggCCGTGGCGGCAATCACCGCCATGGCCCCGGTGCCAACCTGGCCCTGGTGCCCCAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCGGGTTGACGATTTCGAACTGGCTCACCAGGCGCATCTgg  >  5:56662/5‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                            
TTACCAGCTCTGCAGCACCCGGCACATCCTGCACCTTGGGGCCGCGGGTGAGGGTGGCCATACCGTCCTGCACGGTGCCGATGTTTTCGAACAGCGAGGTAATTTCCCACATGATCCAGTGCGACATACCGTTGACGCGCAAGGCCATGGCGGTGATTGCCGCCACGGCCCCGGTGCCAACCTGGCCCTGGTGCCACAGCCACAGTGCGTAGCCACCGGCACCAAAGATCAAGCCCACCACCAACGCCTGGTTGACGATTTCGAACTGGCTCACCAGGCGCATCTGG  >  NC_002947/3007906‑3008192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑