Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,021,984 A→G 7.7% E841G (GAG→GGG) ‡ PP_2638 → cellulose synthase operon protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,021,9840AG 7.7% 149.8 / 2.7 52E841G (GAG→GGG) ‡PP_2638cellulose synthase operon protein C
Reads supporting (aligned to +/- strand):  ref base A (24/24);  new base G (2/2);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01

CATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCGTTGGGTTGGCAGGG  >  NC_002947/3021836‑3022125
                                                                                                                                                    |                                                                                                                                             
cATGACTTCGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGGTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGa                                                                                                                                               <  3:145499/149‑1 (MQ=255)
cATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGa                                                                                                                                               <  8:69326/149‑1 (MQ=255)
      tACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGaa                                                                                                                                         >  4:86036/1‑149 (MQ=255)
          gCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGGCTCGAACGac                                                                                                                                     <  2:255718/149‑2 (MQ=255)
           cTGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGAt                                                                                                                                     >  3:344744/1‑148 (MQ=255)
              gcgcGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGGCTCGAACGaccc                                                                                                                                   <  8:167363/147‑4 (MQ=255)
               cgcgAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCATTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAgg                                                                                                                                 <  7:355060/148‑1 (MQ=255)
                gcgAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAggg                                                                                                                                >  3:127818/1‑148 (MQ=255)
                gcgAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATCCGGGGTCGTTCGAGCCGAACGATCAgg                                                                                                                                 >  8:355060/1‑147 (MQ=255)
                  gAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGCTCAGGGAAg                                                                                                                             >  8:254679/1‑149 (MQ=255)
                      aTGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTac                                                                                                                         <  3:367185/149‑1 (MQ=255)
                         aCGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACAcc                                                                                                                      <  6:332788/149‑1 (MQ=255)
                                 cccGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCTAGCCGAACGATCAGGGAAGCTACACCCCCGACtt                                                                                                              <  5:27373/149‑1 (MQ=255)
                                  ccGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGTCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTc                                                                                                             <  2:120881/149‑1 (MQ=255)
                                   cGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCg                                                                                                            <  4:127818/149‑1 (MQ=255)
                                   cGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCg                                                                                                            <  4:344744/149‑1 (MQ=255)
                                             gAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGc                                                                                                    >  2:79093/1‑147 (MQ=255)
                                             gAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCa                                                                                                  <  4:360823/149‑1 (MQ=255)
                                              aaCTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAg                                                                                                 >  8:241865/1‑149 (MQ=255)
                                                   aGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTttgt                                                                                            >  7:337806/1‑149 (MQ=255)
                                                    gTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTttgtt                                                                                           <  5:251560/149‑1 (MQ=255)
                                                     tACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCt                                                                                                      <  4:243031/137‑1 (MQ=255)
                                                     tACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCt                                                                                                      >  3:243031/1‑137 (MQ=255)
                                                     tACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTttgtt                                                                                           <  3:123850/148‑1 (MQ=255)
                                                         cAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCa                                                                                      <  3:86036/149‑1 (MQ=255)
                                                              gACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCg                                                                                 >  5:303431/1‑149 (MQ=255)
                                                                ccTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCt                                                                               <  6:303431/149‑1 (MQ=255)
                                                                     ttgCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACgg                                                                          <  4:177474/149‑1 (MQ=255)
                                                                         aTGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCt                                                                                                                           <  5:288456/96‑1 (MQ=255)
                                                                         aTGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCt                                                                                                                           >  6:288456/1‑96 (MQ=255)
                                                                          tGTCGACACACCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCAGACCGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCGAGTTTGTTGGCCAGACCGCGGCCGGGaaaa                                                                     >  6:216622/1‑149 (MQ=255)
                                                                                 acCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGcc                                                              >  6:72432/1‑149 (MQ=255)
                                                                                   cccTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCcg                                                            >  1:201402/1‑149 (MQ=255)
                                                                                        ggCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGt                                                         <  7:241865/147‑1 (MQ=255)
                                                                                          cAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCAc                                                     >  7:159942/1‑149 (MQ=255)
                                                                                           aggaggGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACg                                                    >  5:228952/1‑149 (MQ=255)
                                                                                              aggGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACgggg                                                 <  8:337806/149‑1 (MQ=255)
                                                                                                               cgAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCaaa                                <  1:79093/149‑1 (MQ=255)
                                                                                                                     gagCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAg                          >  5:269151/1‑149 (MQ=255)
                                                                                                                     gagCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAg                          >  7:2929/1‑149 (MQ=255)
                                                                                                                          cATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGt                     >  8:171337/1‑149 (MQ=255)
                                                                                                                            ttGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTg                   >  3:51648/1‑149 (MQ=255)
                                                                                                                               gCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACggg                                                  >  5:214081/1‑115 (MQ=255)
                                                                                                                               gCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACggg                                                  <  6:214081/115‑1 (MQ=255)
                                                                                                                                  tGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCgttg             <  4:51648/149‑1 (MQ=255)
                                                                                                                                       gCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCgttgggttg        >  3:357796/1‑149 (MQ=255)
                                                                                                                                        cGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCgttgggttgg       <  1:262619/149‑1 (MQ=255)
                                                                                                                                         ggggTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCgttgggttgg       >  3:308798/1‑148 (MQ=255)
                                                                                                                                         ggggTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCGTTGGGTTggc      <  2:321845/149‑1 (MQ=255)
                                                                                                                                         ctgatCGTTCGGCTCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCgttgggttgg       >  2:71972/5‑148 (MQ=255)
                                                                                                                                          tgatCGTTCGGCTCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCgttgggttgg       >  1:252604/4‑147 (MQ=255)
                                                                                                                                          gggTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCGTTGGGTTggca     <  6:269151/149‑1 (MQ=255)
                                                                                                                                             tCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCGTTGGGTTggcaggg  <  7:171337/149‑1 (MQ=255)
                                                                                                                                               gTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCGTTGGGTTggcaggg  >  4:286909/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CATGACTACGGCTGGCGCGAGGATGACGGTACGCCCGGTACTTCGGAACTGAGTACCCAGTCGACCTTGTTGCATGTCGACACCCCTTGGCAGGAGGGCACCGCGTTTGCGCGAGTAGAGCGCATTGGCATGGATGCGGGGTCGTTCGAGCCGAACGATCAGGGAAGCTACACCCCCGACTTCGGCAGCTGCCAGTTTGTTGGCCAGACCGCTGACGGCAAAACACTGCCCGCGTGCACGGGGGGATCGCAGACTGCAAACGGCAGCCTGTTGGCGTTGGGTTGGCAGGG  >  NC_002947/3021836‑3022125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑