Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,031,004 C→T 7.4% T104I (ACC→ATC)  mgtA → ATP‑dependent magnesium transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,031,0040CT 7.4% 159.6 / 2.3 54T104I (ACC→ATC) mgtAATP‑dependent magnesium transporter
Reads supporting (aligned to +/- strand):  ref base C (27/23);  new base T (2/2);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.53e-01

AGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTC  >  NC_002947/3030869‑3031148
                                                                                                                                       |                                                                                                                                                
aGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATc                                                                                                                                     <  3:176529/149‑1 (MQ=255)
      tgcccGAGCACGACGCCGCCTAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGATCCCGGCCAGGGTCAGcagt                                                                                                                               <  8:184216/146‑4 (MQ=255)
          cGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCa                                                                                                                           >  7:3704/1‑149 (MQ=255)
                 gAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTc                                                                                                                               >  1:85098/1‑138 (MQ=255)
                 gAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTc                                                                                                                               <  2:85098/138‑1 (MQ=255)
                  aaGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGactac                                                                                                                   >  3:273630/1‑149 (MQ=255)
                     ccgccAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTgg                                                                                                                <  8:175402/149‑1 (MQ=255)
                       gccAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCt                                                                                                              >  2:193565/1‑149 (MQ=255)
                               ccTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCa                                                                                                      <  2:108915/149‑1 (MQ=255)
                               ccTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGCCCATGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGCCTACTGGCTGCCGGTCa                                                                                                      >  4:212555/1‑149 (MQ=255)
                                   gTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAgc                                                                                                    <  6:352518/147‑1 (MQ=255)
                                      cgcgATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGACGGTCAGCGCAgg                                                                                               >  5:24330/1‑149 (MQ=255)
                                             gggCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCa                                                                                        <  3:236319/149‑1 (MQ=255)
                                               gCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAga                                                                                      >  4:334706/1‑149 (MQ=255)
                                                  aaCCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAgacga                                                                                   <  2:329910/149‑1 (MQ=255)
                                                    ccAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGacgacg                                                                                 >  7:11482/1‑149 (MQ=255)
                                                            cccACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCGGACGACGCCGACCt                                                                          >  1:73726/1‑148 (MQ=255)
                                                             ccACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGATCCCGGCCAGGGTCAGca                                                                                                                                 <  7:244765/92‑2 (MQ=255)
                                                             ccACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGATCCCGGCCAGGGTCAGca                                                                                                                                 >  8:244765/1‑91 (MQ=255)
                                                             ccACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCt                                                                          <  3:101629/147‑1 (MQ=255)
                                                             ccACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCt                                                                          >  4:101629/1‑147 (MQ=255)
                                                                        aaCCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCACGCGAAGCAGACGACGCCGACCTGACCAAGGtcatc                                                             <  8:11482/149‑1 (MQ=255)
                                                                          ccccACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAga                                                                                      <  6:281651/122‑1 (MQ=255)
                                                                          ccccACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAga                                                                                      >  5:281651/1‑122 (MQ=255)
                                                                          ccccACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGtcatcat                                                           <  4:273630/149‑1 (MQ=255)
                                                                            ccACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGtcatcatca                                                         <  1:1905/149‑1 (MQ=255)
                                                                                  ccTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACTGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGtcat                                                              <  4:186620/138‑1 (MQ=255)
                                                                                  ccTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCGGACGACGCCGACCTGACCAAGGTCATCATCATCATGa                                                   <  2:73726/149‑1 (MQ=255)
                                                                                  ccTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACAGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGtcat                                                              >  3:186620/1‑138 (MQ=255)
                                                                                        gCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAgac                                                                                     >  5:229105/1‑109 (MQ=255)
                                                                                        gCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAgac                                                                                     <  6:229105/109‑1 (MQ=255)
                                                                                        gCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATgg                                             >  3:30016/1‑149 (MQ=255)
                                                                                         cAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGt                                            >  5:346801/1‑149 (MQ=255)
                                                                                           aCTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCa                                          <  8:3704/149‑1 (MQ=255)
                                                                                              gCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGcc                                       >  6:235658/1‑149 (MQ=255)
                                                                                                  aaGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTcag                                   >  6:379540/1‑149 (MQ=255)
                                                                                                  aaGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTc                                     <  3:334706/147‑1 (MQ=255)
                                                                                                       ccTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGcc                              >  6:114431/1‑149 (MQ=255)
                                                                                                       ccTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGcc                              >  2:152035/1‑149 (MQ=255)
                                                                                                           aacaacCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCctgc                           <  4:30016/148‑1 (MQ=255)
                                                                                                              aacCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCtgctgc                        >  5:134135/1‑148 (MQ=255)
                                                                                                                cccGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGAt                     >  5:268787/1‑149 (MQ=255)
                                                                                                                     tCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTgg                >  8:214044/1‑149 (MQ=255)
                                                                                                                      cATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTgg                <  1:193565/148‑1 (MQ=255)
                                                                                                                       aTCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATgg                                             <  8:42735/118‑1 (MQ=255)
                                                                                                                       aTCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATgg                                             >  7:42735/1‑118 (MQ=255)
                                                                                                                       aTCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTgg                >  5:5622/1‑147 (MQ=255)
                                                                                                                            tGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAg             <  5:379540/145‑1 (MQ=255)
                                                                                                                            tGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAg          >  5:29002/1‑148 (MQ=255)
                                                                                                                               gaagctgATCCCGGCCAGGGTCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCCTGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTAcc      >  4:109129/4‑149 (MQ=255)
                                                                                                                                ctgctgACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGc                                                                                >  7:174299/1‑74 (MQ=255)
                                                                                                                                ctgctgACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGc                                                                                <  8:174299/74‑1 (MQ=255)
                                                                                                                                   ctgACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTc  <  5:235658/149‑1 (MQ=255)
                                                                                                                                   ctgACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTc  >  1:166078/1‑149 (MQ=255)
                                                                                                                                       |                                                                                                                                                
AGGGCTTGACCGAGCACGAAGCCGCCAAGCGCCTGGTGCGCGATGGGGCCAACCAGGTTGCCCACGACCCGCAACCCCACGCCCTGGTGCAACTGCTCAAGGCCCTGAACAACCCGTTCATCTATGTACTGCTGACCCTGGCCGGGATCAGCTTCGTCACCGACTACTGGCTGCCGGTCAGCGCAGGCGAAGCAGACGACGCCGACCTGACCAAGGTCATCATCATCATGACCATGGTCAGCCTCAGCAGCCTGCTGCGATTCTGGCAGGAGTACCGCTC  >  NC_002947/3030869‑3031148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑