Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,034,904	C→G	7.2%	L95L (CTG→CTC) ‡	PP_2647 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,034,904	0	C	G	7.2%	221.2 / 4.8	69	L95L (CTG→CTC) ‡	PP_2647	MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (34/30);  new base G (3/2);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

CGTCAGGTGCCAGGCGACCGCTGAGGGCGATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTGA  >  NC_002947/3034759‑3035049                                                                                                                                                   |                                                                                                                                                  cGTCAGGTGCCAGGCGACCGCTGAGGGCGATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAgg                                                                                                                                                <  3:335046/149‑1 (MQ=255) cGTCAGGTGCCAGGCGACCGCTGAGGGCGATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAgg                                                                                                                                                <  3:329701/149‑1 (MQ=255)        tGCCAGGCGACCGCTGAGGGCGATGGCTGTCGCCCAGAAGCCACCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGAt                                                                                                                                           >  3:198286/1‑147 (MQ=255)                           gCGATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAg                                                                                                                      <  4:198531/149‑1 (MQ=255)                             gATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATcagca                                                                                                                                      >  5:149622/1‑131 (MQ=255)                             gATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATcagca                                                                                                                                      <  6:149622/131‑1 (MQ=255)                                      cGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATc                                                                                                                                          <  5:153545/118‑1 (MQ=255)                                      cGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATc                                                                                                                                          >  6:153545/1‑118 (MQ=255)                                       gCCCAGAAGCCTCCAATACTGATACCCAGTAGCACACGGCCGACCCACAGCAGGTTGAAGTCACCGGCATAGGCCCCGAGGGCGTTGGCGATGATCCTGCTCAGC‑GTCAGGCCGATCCGCAGGTAGCGCAGGTCCAGCGcgccgccgca                                                                                                          >  2:24068/1‑148 (MQ=255)                                              aGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGaa                                                                                                   <  6:1078/149‑1 (MQ=255)                                                  gccAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATc                                                                                                >  4:238372/1‑148 (MQ=255)                                                   ccAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAATTCac                                                                                              >  4:207099/1‑148 (MQ=255)                                                    cAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAAc                                                                                             >  6:81974/1‑149 (MQ=255)                                                          tGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGggcg                                                                                       >  3:7871/1‑149 (MQ=255)                                                          tGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAAAGGggcg                                                                                       >  3:248363/1‑149 (MQ=255)                                                          tGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGA‑GTCAGGCCGAGCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGggcg                                                                                       >  7:129750/1‑149 (MQ=255)                                                           gATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGggcgg                                                                                      >  3:359857/1‑149 (MQ=255)                                                            aTACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGggcgg                                                                                      <  6:169368/148‑1 (MQ=255)                                                             tACACAGTAGCACACTGCCCACCAACAGCAGGTTGAAGTCACCGCCATAGGCCACTATGGCGATGGCGATGATAATGATCAGA‑GTCACTCCGATCCGCAGGTAGCGACGGTCCCGCGCGCAGAAGCCTACCGAAATCAACGGggccgc                                                                                     >  6:370505/1‑148 (MQ=255)                                                               cccAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTaa                                                                                  <  6:139309/149‑1 (MQ=255)                                                                gcAGTAGCACACGGCCGACCAACAGCAGGTTGCAGTGACCGGCATAGGCCAGGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAAc                                                                                             <  8:103440/136‑1 (MQ=255)                                                                ccAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGCCGCCTACGGAAATCAAc                                                                                             >  7:103440/1‑137 (MQ=255)                                                                ccAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAAc                                                                                 >  1:70782/1‑149 (MQ=255)                                                                 cAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGaaa                                                                                                  >  5:231416/1‑131 (MQ=255)                                                                 cAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGaaa                                                                                                  <  6:231416/131‑1 (MQ=255)                                                                  aGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACg                                                                                >  3:342395/1‑148 (MQ=255)                                                                    tAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAg                                                                             <  4:198286/149‑1 (MQ=255)                                                                      gCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGc                                                                            <  8:243037/148‑1 (MQ=255)                                                                      gCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGc                                                                            <  4:261814/148‑1 (MQ=255)                                                                       cacaCGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCa                                                                          >  3:275647/1‑149 (MQ=255)                                                                                 aCCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTcc                                                                                                                        <  4:323032/93‑1 (MQ=255)                                                                                      cagcagGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGCGGTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAgg                                                            >  1:106722/1‑149 (MQ=255)                                                                                         cagGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCa                                                        <  5:7127/149‑1 (MQ=255)                                                                                          agGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCAc                                                       >  5:122907/1‑149 (MQ=255)                                                                                           ggTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCAcc                                                      <  5:81974/149‑1 (MQ=255)                                                                                            gTTGAAGTCACCGGCATAGGCCACGATGGGGTTGGGGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCAcc                                                      <  2:278206/148‑1 (MQ=255)                                                                                            gTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCa                                                     >  1:246191/1‑149 (MQ=255)                                                                                                aaGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAg                                                 <  3:169703/149‑1 (MQ=255)                                                                                                  gTCACCGGCATAGGCCACGAAGGCGTTGGCGAAGAACATCATCACC‑GTCAGGCCGAACAGCAGGTAGCGCCGGTCCAGCGCCCCGACCCCTACGGAAATCAACGGGGCCGCTAACGCAGCCATGATCCCGGGAAGGGTCACCATCAgcc                                               >  3:346159/1‑147 (MQ=255)                                                                                                   tCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGcc                                              >  6:80007/1‑149 (MQ=255)                                                                                                   tCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGcc                                              >  2:193898/1‑149 (MQ=255)                                                                                                     aCCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGCGGACAGCCCGATCAGAAGGTAGCGCCGGACCACCGCGCCGACGCGACCGAAAATCAACGGGCCGGATAACCCAGCAATGATGCCGGAAAGGTACCACAACAGGCCg                                             >  1:259284/1‑149 (MQ=255)                                                                                                     aCCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCgg                                            >  8:69183/1‑149 (MQ=255)                                                                                                     aCCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCgg                                            >  3:9899/1‑149 (MQ=255)                                                                                                      ccGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGc                                           <  2:259284/149‑1 (MQ=255)                                                                                                         gCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGCTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAg                                        <  1:52771/149‑1 (MQ=255)                                                                                                               gCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGc                                      >  5:87953/1‑145 (MQ=255)                                                                                                               gCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGc                                      <  6:87953/145‑1 (MQ=255)                                                                                                                ccACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGc                                  >  7:239587/1‑148 (MQ=255)                                                                                                                 cACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCAc                                >  7:224062/1‑149 (MQ=255)                                                                                                                    gATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGa                             >  3:38934/1‑149 (MQ=255)                                                                                                                     aTGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGAt                            <  4:7871/149‑1 (MQ=255)                                                                                                                          gTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGcc                         >  3:180113/1‑147 (MQ=255)                                                                                                                              gCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGt                    <  8:224062/148‑1 (MQ=255)                                                                                                                                gatgatCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGc                 >  4:279179/1‑149 (MQ=255)                                                                                                                                    atcatgctcaGC‑GTCAGGCCGATCAGCCGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCACCGGGGCGGCTAACGCAGCCAGGATGCCGGGAAGGGTCACCAGCAGGCCGGCAAGGCCGGCACTGATGCCCAGGTCGCggg              <  1:24068/148‑1 (MQ=255)                                                                                                                                     tgctgatcgGC‑CTGACGCTGATCAGCAGGTAGCGCCGGTCCCGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGc             >  2:305246/4‑148 (MQ=255)                                                                                                                                     tgctgatcgGC‑CTGACGCTGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGccc                        >  7:189107/4‑137 (MQ=255)                                                                                                                                     tgctgatcgGC‑CTGACGCTGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGccc                        <  8:189107/134‑1 (MQ=255)                                                                                                                                     tcatgatcaGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGc             >  3:223564/1‑148 (MQ=255)                                                                                                                                     tcatgatcaGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAAAGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGc             >  3:306056/1‑148 (MQ=255)                                                                                                                                      gctgatcgGC‑GTGACGCTGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCatga                                                                       >  3:72808/3‑89 (MQ=255)                                                                                                                                      gctgatcgGC‑CTGACGCTGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCatga                                                                       <  4:72808/87‑1 (MQ=38)                                                                                                                                             aGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGtt    <  4:342395/149‑1 (MQ=255)                                                                                                                                             aGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGtt    <  6:122907/149‑1 (MQ=255)                                                                                                                                             aGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGtt    <  7:320495/149‑1 (MQ=255)                                                                                                                                             aGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGtt    <  1:193898/149‑1 (MQ=255)                                                                                                                                              gC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTg   <  4:38934/149‑1 (MQ=255)                                                                                                                                               c‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTGa  <  5:370505/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CGTCAGGTGCCAGGCGACCGCTGAGGGCGATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTGA  >  NC_002947/3034759‑3035049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑