Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,034,909	C→T	6.9%	G94S (GGC→AGC)	PP_2647 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,034,909	0	C	T	6.9%	210.3 / 4.0	72	G94S (GGC→AGC)	PP_2647	MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (36/31);  new base T (3/2);  total (39/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

TGCCAGGCGACCGCTGAGGGCGATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTGAGTACCC  >  NC_002947/3034766‑3035055                                                                                                                                                 |                                                                                                                                                   tGCCAGGCGACCGCTGAGGGCGATGGCTGTCGCCCAGAAGCCACCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGAt                                                                                                                                                 >  3:198286/1‑147 (MQ=255)                    gCGATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAg                                                                                                                            <  4:198531/149‑1 (MQ=255)                      gATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATcagca                                                                                                                                            <  6:149622/131‑1 (MQ=255)                      gATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATcagca                                                                                                                                            >  5:149622/1‑131 (MQ=255)                               cGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATc                                                                                                                                                >  6:153545/1‑118 (MQ=255)                               cGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATc                                                                                                                                                <  5:153545/118‑1 (MQ=255)                                gCCCAGAAGCCTCCAATACTGATACCCAGTAGCACACGGCCGACCCACAGCAGGTTGAAGTCACCGGCATAGGCCCCGAGGGCGTTGGCGATGATCCTGCTCAGC‑GTCAGGCCGATCCGCAGGTAGCGCAGGTCCAGCGcgccgccgca                                                                                                                >  2:24068/1‑148 (MQ=255)                                       aGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGaa                                                                                                         <  6:1078/149‑1 (MQ=255)                                           gccAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATc                                                                                                      >  4:238372/1‑148 (MQ=255)                                            ccAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAATTCac                                                                                                    >  4:207099/1‑148 (MQ=255)                                             cAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAAc                                                                                                   >  6:81974/1‑149 (MQ=255)                                                   tGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGggcg                                                                                             >  3:7871/1‑149 (MQ=255)                                                   tGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAAAGGggcg                                                                                             >  3:248363/1‑149 (MQ=255)                                                   tGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGA‑GTCAGGCCGAGCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGggcg                                                                                             >  7:129750/1‑149 (MQ=255)                                                    gATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGggcgg                                                                                            >  3:359857/1‑149 (MQ=255)                                                     aTACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGggcgg                                                                                            <  6:169368/148‑1 (MQ=255)                                                      tACACAGTAGCACACTGCCCACCAACAGCAGGTTGAAGTCACCGCCATAGGCCACTATGGCGATGGCGATGATAATGATCAGA‑GTCACTCCGATCCGCAGGTAGCGACGGTCCCGCGCGCAGAAGCCTACCGAAATCAACGGggccgc                                                                                           >  6:370505/1‑148 (MQ=255)                                                        cccAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTaa                                                                                        <  6:139309/149‑1 (MQ=255)                                                         gcAGTAGCACACGGCCGACCAACAGCAGGTTGCAGTGACCGGCATAGGCCAGGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAAc                                                                                                   <  8:103440/136‑1 (MQ=255)                                                         ccAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGCCGCCTACGGAAATCAAc                                                                                                   >  7:103440/1‑137 (MQ=255)                                                         ccAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAAc                                                                                       >  1:70782/1‑149 (MQ=255)                                                          cAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGaaa                                                                                                        >  5:231416/1‑131 (MQ=255)                                                          cAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGaaa                                                                                                        <  6:231416/131‑1 (MQ=255)                                                           aGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACg                                                                                      >  3:342395/1‑148 (MQ=255)                                                             tAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAg                                                                                   <  4:198286/149‑1 (MQ=255)                                                               gCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGc                                                                                  <  4:261814/148‑1 (MQ=255)                                                               gCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGc                                                                                  <  8:243037/148‑1 (MQ=255)                                                                cacaCGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCa                                                                                >  3:275647/1‑149 (MQ=255)                                                                          aCCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTcc                                                                                                                              <  4:323032/93‑1 (MQ=255)                                                                               cagcagGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGCGGTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAgg                                                                  >  1:106722/1‑149 (MQ=255)                                                                                  cagGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCa                                                              <  5:7127/149‑1 (MQ=255)                                                                                   agGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCAc                                                             >  5:122907/1‑149 (MQ=255)                                                                                    ggTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCAcc                                                            <  5:81974/149‑1 (MQ=255)                                                                                     gTTGAAGTCACCGGCATAGGCCACGATGGGGTTGGGGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCAcc                                                            <  2:278206/148‑1 (MQ=255)                                                                                     gTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCa                                                           >  1:246191/1‑149 (MQ=255)                                                                                         aaGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAg                                                       <  3:169703/149‑1 (MQ=255)                                                                                           gTCACCGGCATAGGCCACGAAGGCGTTGGCGAAGAACATCATCACC‑GTCAGGCCGAACAGCAGGTAGCGCCGGTCCAGCGCCCCGACCCCTACGGAAATCAACGGGGCCGCTAACGCAGCCATGATCCCGGGAAGGGTCACCATCAgcc                                                     >  3:346159/1‑147 (MQ=255)                                                                                            tCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGcc                                                    >  6:80007/1‑149 (MQ=255)                                                                                            tCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGcc                                                    >  2:193898/1‑149 (MQ=255)                                                                                              aCCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGCGGACAGCCCGATCAGAAGGTAGCGCCGGACCACCGCGCCGACGCGACCGAAAATCAACGGGCCGGATAACCCAGCAATGATGCCGGAAAGGTACCACAACAGGCCg                                                   >  1:259284/1‑149 (MQ=255)                                                                                              aCCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCgg                                                  >  8:69183/1‑149 (MQ=255)                                                                                              aCCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCgg                                                  >  3:9899/1‑149 (MQ=255)                                                                                               ccGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGc                                                 <  2:259284/149‑1 (MQ=255)                                                                                                  gCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGCTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAg                                              <  1:52771/149‑1 (MQ=255)                                                                                                        gCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGc                                            >  5:87953/1‑145 (MQ=255)                                                                                                        gCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGc                                            <  6:87953/145‑1 (MQ=255)                                                                                                         ccACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGc                                        >  7:239587/1‑148 (MQ=255)                                                                                                          cACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCAc                                      >  7:224062/1‑149 (MQ=255)                                                                                                             gATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGa                                   >  3:38934/1‑149 (MQ=255)                                                                                                              aTGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGAt                                  <  4:7871/149‑1 (MQ=255)                                                                                                                   gTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGcc                               >  3:180113/1‑147 (MQ=255)                                                                                                                       gCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGt                          <  8:224062/148‑1 (MQ=255)                                                                                                                         gatgatCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGc                       >  4:279179/1‑149 (MQ=255)                                                                                                                             atcatgctcaGC‑GTCAGGCCGATCAGCCGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCACCGGGGCGGCTAACGCAGCCAGGATGCCGGGAAGGGTCACCAGCAGGCCGGCAAGGCCGGCACTGATGCCCAGGTCGCggg                    <  1:24068/148‑1 (MQ=255)                                                                                                                              tgctgatcgGC‑CTGACGCTGATCAGCAGGTAGCGCCGGTCCCGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGc                   >  2:305246/4‑148 (MQ=255)                                                                                                                              tgctgatcgGC‑CTGACGCTGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGccc                              <  8:189107/134‑1 (MQ=255)                                                                                                                              tgctgatcgGC‑CTGACGCTGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGccc                              >  7:189107/4‑137 (MQ=255)                                                                                                                              tcatgatcaGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGc                   >  3:223564/1‑148 (MQ=255)                                                                                                                              tcatgatcaGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAAAGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGc                   >  3:306056/1‑148 (MQ=255)                                                                                                                               gctgatcgGC‑GTGACGCTGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCatga                                                                             >  3:72808/3‑89 (MQ=255)                                                                                                                               gctgatcgGC‑CTGACGCTGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCatga                                                                             <  4:72808/87‑1 (MQ=38)                                                                                                                                      aGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGtt          <  7:320495/149‑1 (MQ=255)                                                                                                                                      aGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGtt          <  4:342395/149‑1 (MQ=255)                                                                                                                                      aGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGtt          <  6:122907/149‑1 (MQ=255)                                                                                                                                      aGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGtt          <  1:193898/149‑1 (MQ=255)                                                                                                                                       gC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTg         <  4:38934/149‑1 (MQ=255)                                                                                                                                        c‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTGa        <  5:370505/149‑1 (MQ=255)                                                                                                                                             aGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTGAGTa     <  8:239587/148‑1 (MQ=255)                                                                                                                                             aGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTGAGTAc    <  5:80007/149‑1 (MQ=255)                                                                                                                                             aGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTGAGTAc    >  8:263349/1‑149 (MQ=255)                                                                                                                                              gtccGATGAGCAGGTTGCGCCGTTCCAGCGCGCCGTCGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCTAGGCCTGCAGTGATGCCCAGGTCGCTGTCCACATCGTTGAGTAcc   <  4:346159/147‑1 (MQ=255)                                                                                                                                              ggCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTGAGTAcc   <  2:70782/149‑1 (MQ=255)                                                                                                                                               gCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTGGAGTAccc  >  2:308662/1‑149 (MQ=255)                                                                                                                                                 cGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCcac                >  4:307755/1‑133 (MQ=255)                                                                                                                                                 cGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCcac                <  3:307755/133‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   TGCCAGGCGACCGCTGAGGGCGATGGCTGTCGCCCAGAAGCCGCCAATACTGATACCCAGTAGCACACGGCCGACCAACAGCAGGTTGAAGTCACCGGCATAGGCCACGATGGCGTTGGCGATGATCATGATCAGC‑GTCAGGCCGATCAGCAGGTAGCGCCGGTCCAGCGCGCCGACGCCTACGGAAATCAACGGGGCGGCTAACGCAGCCATGATGCCGGGAAGGGTCACCATCAGGCCGGCAAGGCCTGCACTGATGCCCAGGTCGCTGGCCACATCGTTGAGTACCC  >  NC_002947/3034766‑3035055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑