Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,048,605	A→C	10.2%	intergenic (+60/‑236)	PP_2661 → / → PP_2662	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,048,605	0	A	C	10.2%	176.3 / 10.4	67	intergenic (+60/‑236)	PP_2661/PP_2662	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/32);  new base C (3/4);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

GTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTCCCGCCCCAAA  >  NC_002947/3048459‑3048748                                                                                                                                                   |                                                                                                                                                  gtggATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGc                                                                                                                                                 >  6:313731/1‑149 (MQ=255)      tgtgCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCcgc                                                                                                                                             >  2:119342/1‑148 (MQ=255)        tgCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGCAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCTCGCCCGCGaa                                                                                                                                          <  1:230191/149‑1 (MQ=255)        tgCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCGGCCCTTCGCGGGCTCGCCCGCGaa                                                                                                                                          <  4:30204/149‑1 (MQ=255)         gCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAg                                                                                                                                         >  1:43568/1‑149 (MQ=255)          cAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCCGCCTTGCGGCCCTTCGCGGGCTCGCCCGCGAAg                                                                                                                                         <  4:281022/148‑1 (MQ=39)            ggCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGc                                                                                                                                      >  4:11747/1‑149 (MQ=255)             gCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGcc                                                                                                                                     >  5:93974/1‑149 (MQ=255)             gCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGcc                                                                                                                                     <  4:98613/149‑1 (MQ=255)                   ctggcctggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAgg                                                                                                                               <  5:272114/149‑1 (MQ=255)                          ggcAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGcc‑cc                                                                                                                       >  6:297535/1‑149 (MQ=255)                             agcaACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGt                                                                                                                    <  2:3665/149‑1 (MQ=255)                                aaCCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGATTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCATTGCAGCACTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGttca                                                                                                                 >  3:46733/1‑149 (MQ=255)                                  ccGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGttcatt                                                                                                               <  1:357435/149‑1 (MQ=255)                                     cACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCaa                                                                                                            <  2:300813/149‑1 (MQ=255)                                     cACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCaa                                                                                                            >  5:114601/1‑149 (MQ=255)                                       ccGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATc                                                                                                          <  3:11747/149‑1 (MQ=255)                                         gTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCg                                                                                                         >  3:174493/1‑148 (MQ=255)                                          ttATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCT‑CCGGTTCATTCAATCGTa                                                                                                       <  6:93974/149‑1 (MQ=255)                                          ttATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTa                                                                                                       <  2:278361/149‑1 (MQ=255)                                            aTGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTAtt                                                                                                     >  1:149315/1‑149 (MQ=255)                                              gAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGa                                                                                                   >  1:288250/1‑149 (MQ=255)                                               aaaTGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGa                                                                                                   <  8:24495/148‑1 (MQ=255)                                                aaTGCCCGCTTGCCAGAGTTCGCAGGCACTGGCTTCCTGAACCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATa                                                                                                 >  2:55993/1‑149 (MQ=255)                                                 aTGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATAc                                                                                                <  7:93913/149‑1 (MQ=255)                                                      ccagtGCCTGCGAACGCTGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑cc                                                                                          >  8:111132/5‑149 (MQ=255)                                                         gtGCCTGCGAACGCTGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGAGAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGACGGGCCGCAAGGCGGCC‑CCGGTTCTTTCAATCGTATTGATACACG‑CCTGc                                                                                       >  4:296686/2‑149 (MQ=255)                                                           gCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGc                                                                                       <  2:43568/147‑1 (MQ=255)                                                            ccAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCa                                                                                     <  7:26421/148‑1 (MQ=255)                                                                  ttCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTcctgc                                                                              >  8:92174/1‑149 (MQ=255)                                                                    cGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTcctgcct                                                                            >  5:54634/1‑149 (MQ=255)                                                                     gCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCAGTCAATCGTATTGATACCCG‑CCTGCCACTcctgcct                                                                            >  4:277773/1‑148 (MQ=255)                                                                       aGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAg                                                                         <  8:170169/149‑1 (MQ=255)                                                                           aCTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCAt                                                                     <  5:297535/149‑1 (MQ=255)                                                                             tgtcTTCCTGAAGCCAGGAGATTCCGCTCTGTTAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCTCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑GCTGCCACTCCTGCCTGAGCCATaa                                                                   <  3:265195/146‑1 (MQ=255)                                                                               gCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATaa                                                                   <  4:174493/147‑1 (MQ=255)                                                                               gCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGt                                                                 >  5:40771/1‑149 (MQ=255)                                                                                 ttCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAAACGTATTGATACACG‑CCTGCCACTCCTGCCTGAGCCATAAGtct                                                               >  7:251116/1‑149 (MQ=255)                                                                                   ccTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGcc                                                                       <  2:242419/139‑1 (MQ=255)                                                                                   ccTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGcc                                                                       >  1:242419/1‑139 (MQ=255)                                                                                     tGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATaa                                                                   >  4:288444/1‑141 (MQ=255)                                                                                     tGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATaa                                                                   <  3:288444/141‑1 (MQ=255)                                                                                      gAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCCGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGACATAACACTCGAAc                                                          >  1:59275/1‑149 (MQ=255)                                                                                       aaGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAACGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAAc                                                          <  1:119342/148‑1 (MQ=255)                                                                                        aGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCa                                                        <  5:313731/149‑1 (MQ=255)                                                                                         tccATGATATTCCTCTAAGATAATGTAATTGTGGCTGCTTTGCAGCCCTTCGCGGTCGAGCCCGAGTAGGGCCTCAAGGCGGCC‑CCGTTTCATTAAATCGTATTGATACCCGCCCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCa                                                        <  1:55993/148‑1 (MQ=255)                                                                                         gCCAGGAGAATCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCTCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAg                                                       <  4:46733/149‑1 (MQ=255)                                                                                           cAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGtc                                                                >  2:114448/1‑138 (MQ=255)                                                                                           cAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGtc                                                                <  1:114448/138‑1 (MQ=255)                                                                                             ggAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTAtt                                                                                                     >  7:67533/1‑100 (MQ=255)                                                                                             ggAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTAtt                                                                                                     <  8:67533/100‑1 (MQ=255)                                                                                                      gCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGCGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCAGTCACTCGTATTGATACCCG‑CCTGCCACTCCTGCCTCAGCCATAAGGCGCGAACCAGGACCCAAGCAggg                                          >  4:52904/1‑148 (MQ=255)                                                                                                          aGATAATGTTATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTAc                                        <  2:288250/147‑1 (MQ=255)                                                                                                           gATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCgg                                     <  7:92174/149‑1 (MQ=255)                                                                                                               aTGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGAc                                  >  3:295967/1‑148 (MQ=255)                                                                                                                  ttaTTGGGGCTGCTTTTCAGCCCGTGGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGTTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCaa                               <  8:251116/146‑1 (MQ=255)                                                                                                                   aaTTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCCTGCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCaaa                              >  1:107084/1‑149 (MQ=255)                                                                                                                                 tGCAGCCCTTCGCGGGCGAGCCGGCGAAGGGCCGCAATGCGGCG‑CCGGTTCATTCAATCGTATTGATACCGG‑CCTTCCACTCCTGCCTGAGCCATAAGTCTGGAACCAGGACCCAAGCTGGTACCGGGACCAAATCACCGGGCCGTa                 <  2:59275/147‑1 (MQ=255)                                                                                                                                  gCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTAcc               >  6:125100/1‑148 (MQ=255)                                                                                                                                    ggCCCTTCGCGGGCTCGCCCGCGAAGGGCTGCAAAGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTAc                >  2:43218/2‑145 (MQ=255)                                                                                                                                    ggCCCTTCGCGGGCTCGCCCGCGAAGGGCTGCAAAGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTAc                <  1:43218/144‑1 (MQ=255)                                                                                                                                    ggCCCTTCGCGGGCTCGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTc            >  5:233598/2‑149 (MQ=255)                                                                                                                                        cTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTCCCGc        >  3:83034/1‑149 (MQ=255)                                                                                                                                           cgcgGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTCCCGcccc     <  4:295967/149‑1 (MQ=255)                                                                                                                                              gggCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTCCCGCCCCaaa  <  6:54634/149‑1 (MQ=255)                                                                                                                                              gggCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTCCCGCCCCaaa  <  7:111132/149‑1 (MQ=255)                                                                                                                                              gggCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTCCCGCCCCaaa  <  3:277773/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GTGGATGTGCAGGCACGGCTGGCCTGGCAGCAACCGCACCGTTATGAAATGCCCGCTTGCCAGCGTTCGCAGGCACTGGCTTCCTGAAGCCAGGAGATTCCGCTCAGATAATGTAATTGGGGCTGCTTTGCAGCCCTTCGCGGGCGAGCCCGCGAAGGGCCGCAAGGCGGCC‑CCGGTTCATTCAATCGTATTGATACCCG‑CCTGCCACTCCTGCCTGAGCCATAAGTCTCGAACCAGGACCCAAGCAGGTACCGGGACCAAATCACCGGGCCGTACCCTCCCGCCCCAAA  >  NC_002947/3048459‑3048748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑