Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,146,611 T→C 6.8% intergenic (+47/+19) PP_2761 → / ← sfnA ribose ABC transporter permease/acyl‑CoA dehydrogenase/oxidase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,146,6110TC 6.8% 172.7 / 2.7 59intergenic (+47/+19)PP_2761/sfnAribose ABC transporter permease/acyl‑CoA dehydrogenase/oxidase family protein
Reads supporting (aligned to +/- strand):  ref base T (31/24);  new base C (2/2);  total (33/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

CTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGTTGCG  >  NC_002947/3146463‑3146758
                                                                                                                                                    |                                                                                                                                                   
ctgATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAGCGCCAGTGTCGTGACGAGGCCTGGGGt                                                                                                                                                     <  2:176827/149‑1 (MQ=255)
        cTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGCATACCCCa                                                                                                                                             <  7:130812/149‑1 (MQ=255)
         tGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAg                                                                                                                                            >  5:195905/1‑149 (MQ=255)
         tGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAg                                                                                                                                            >  3:324030/1‑149 (MQ=255)
          ggAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAgg                                                                                                                                           >  5:30764/1‑149 (MQ=255)
           gAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCATTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGc                                                                                                                                          >  7:198553/1‑149 (MQ=255)
             aTTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTTGTGACGAGGCCTGGGGCATACCCCAGGcct                                                                                                                                        <  6:325751/149‑2 (MQ=255)
                    ctGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCttt                                                                                                                                  <  8:238395/148‑1 (MQ=255)
                     tGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGcccc                                                                                                                                              <  1:152275/135‑1 (MQ=255)
                     tGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGcccc                                                                                                                                              >  2:152275/1‑135 (MQ=255)
                       gcagcaGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCa                                                                                                                                <  3:315462/147‑1 (MQ=255)
                       gcagcaGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCa                                                                                                                                <  6:308209/147‑1 (MQ=255)
                       gcagcaGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAg                                                                                                                               <  7:72497/148‑1 (MQ=255)
                       gcagcaGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAg                                                                                                                               >  8:243300/1‑148 (MQ=255)
                       gcagcaGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGCGTATGCCCCAGGCCAAGCTTTCAg                                                                                                                               >  7:191866/1‑148 (MQ=255)
                         agcagGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGAc                                                                                                                            >  3:150430/1‑149 (MQ=255)
                         agcagGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGAc                                                                                                                            <  7:243300/149‑1 (MQ=255)
                                      cGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGc                                                                                                               >  6:186344/1‑149 (MQ=255)
                                      cGGCGTTCTGATTCGCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGc                                                                                                               <  8:261596/149‑1 (MQ=255)
                                                        ggTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCcataca                                                                                              <  5:186344/148‑1 (MQ=255)
                                                          ttCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAg                                                                                           >  8:194210/1‑149 (MQ=255)
                                                          ttCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAg                                                                                           <  3:224729/149‑1 (MQ=255)
                                                               ctgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGggcgg                                                                                      >  4:367372/1‑149 (MQ=255)
                                                                tgTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGggcggc                                                                                     >  6:169401/1‑149 (MQ=255)
                                                                  ttCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGc                                                                                                               >  7:227305/1‑121 (MQ=255)
                                                                  ttCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGc                                                                                                               <  8:227305/121‑1 (MQ=255)
                                                                     aGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCCTGCCTTCAGGACTGCTTCGCACCGACACCGATCTGCCATACATCGGGCGGCTCGcg                                                                                >  4:45354/1‑148 (MQ=255)
                                                                      gTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCa                                                                                                                                             >  8:269624/1‑87 (MQ=255)
                                                                      gTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCa                                                                                                                                             <  7:269624/87‑1 (MQ=255)
                                                                      gTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTg                                                                               >  5:370061/1‑149 (MQ=255)
                                                                       ttttGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGc                                                                              >  1:328994/1‑149 (MQ=255)
                                                                       ttttGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGc                                                                              <  3:367372/149‑1 (MQ=255)
                                                                             cAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCGATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGa                                                                        <  5:128133/149‑1 (MQ=255)
                                                                                   gCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGc                                                                   >  5:85059/1‑148 (MQ=255)
                                                                                   gCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATAGATAGGGCGGCTCGCGGCCCTTGACGTGc                                                                   >  8:251502/1‑148 (MQ=255)
                                                                                        tCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGCCGTGCCAGTCg                                                             >  4:94260/1‑149 (MQ=255)
                                                                                                  aTAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGc                                                   <  7:125348/149‑1 (MQ=255)
                                                                                                    gacacTGGCGTTCCGCCCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCa                                                                 <  2:93997/129‑1 (MQ=255)
                                                                                                    gacacTGGCGTTCCGCCCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCa                                                                 >  1:93997/5‑133 (MQ=255)
                                                                                                      ttCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCga                                                         >  6:306670/1‑139 (MQ=255)
                                                                                                      ttCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCga                                                         <  5:306670/139‑1 (MQ=255)
                                                                                                        cTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTcc                                              <  7:194210/148‑1 (MQ=255)
                                                                                                           gCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTg                                           >  2:251690/1‑148 (MQ=255)
                                                                                                           gCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTg                                           >  4:118047/1‑148 (MQ=255)
                                                                                                           gCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGt                                          <  4:150430/149‑1 (MQ=255)
                                                                                                           gCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCCTg                                           >  6:51473/1‑148 (MQ=255)
                                                                                                            cGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTa                                         <  6:30764/149‑1 (MQ=255)
                                                                                                            cGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTa                                         <  5:169401/149‑1 (MQ=255)
                                                                                                             gTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCTATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTg                                           >  7:149261/1‑146 (MQ=255)
                                                                                                             gTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCAGACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGCTGCGTTCCtgg                                           >  8:85516/1‑144 (MQ=255)
                                                                                                                   ggCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCAcc                                  >  3:325206/1‑149 (MQ=255)
                                                                                                                          cGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTtgt                           <  4:324030/149‑1 (MQ=255)
                                                                                                                                tgtCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAgg                     <  1:251690/149‑1 (MQ=255)
                                                                                                                                  tCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAggcg                   >  6:336742/1‑149 (MQ=255)
                                                                                                                                  tCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAggc                    <  6:195905/148‑1 (MQ=255)
                                                                                                                                    gTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAggcgg                  <  2:328994/148‑1 (MQ=255)
                                                                                                                                          tggCCTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACg           >  6:86743/2‑149 (MQ=255)
                                                                                                                                           ggCCTGGGGCATACCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACgg          >  5:277428/1‑149 (MQ=255)
                                                                                                                                                  ggTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGTTgc   >  7:137601/1‑149 (MQ=255)
                                                                                                                                                   gTATGCCCCAGGCCATGCTTTCAGGACTGCTTTGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGTTgcg  >  2:188710/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CTGATCGGCTGGAATTTCTTCTGGCAGCAGGTGGCCACCGGCGTTCTGATTCTCTCGGTTCTGCTGTTCAGTTTTGCCAGTCGGCGACTCGGCGCTGGATAGTTCTGGCGTTCCGGGCGGAACGCCAGTGTCGTGACGAGGCCTGGGGTATGCCCCAGGCCATGCTTTCAGGACTGCTTGGCACCGCCACCGATCTGCCATACATAGGGCGGCTCGCTGCCATTGACGTGCCAGTCGCCGATGATGCGTTCCTTGTAGATCACCGGGTTGTGCGAGGCGGCAGTACGGGCGTTGCG  >  NC_002947/3146463‑3146758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑