| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 239,052 | C→T | 5.2% | P45S (CCA→TCA) ‡ | hemD → | uroporphyrinogen‑III synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 239,052 | 0 | C | T | 5.2% | 300.5 / 3.6 | 97 | P45S (CCA→TCA) ‡ | hemD | uroporphyrinogen‑III synthase |
| Reads supporting (aligned to +/- strand): ref base C (48/43); new base T (2/3); total (51/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
GCGAGGCCGGTCACCCGTGAGCCAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGAGGCAACCGC > NC_002947/238904‑239200 | gCGAGGCCGGTCACCCGTGAGCCAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑Gccc < 3:185083/149‑1 (MQ=255) gAGGCCGGTCACCCGTGAGCCAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCca > 8:14216/1‑149 (MQ=255) ggTCACCCGTGAGCCAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCgg > 1:206845/1‑149 (MQ=255) ggTCACCCGTGAGCCAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCgg > 2:247100/1‑149 (MQ=255) tCACCCGTGAGCCAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCt < 2:206845/149‑1 (MQ=255) ccGTGAGCCAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCg > 4:195647/1‑149 (MQ=255) tGAGCCAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTg > 4:84296/1‑149 (MQ=255) aGCCAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTgg < 7:216804/148‑1 (MQ=255) ccAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGCGAAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTgg > 1:283912/1‑147 (MQ=255) ccAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAg > 4:303484/1‑149 (MQ=255) aTGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCTTCCAGCGACAGCCGCTgtggggct > 8:127964/1‑142 (MQ=255) gcgcCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTg > 8:303382/1‑148 (MQ=255) gcgcCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGc < 8:344259/149‑1 (MQ=255) gcgcCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGc < 4:156233/149‑1 (MQ=255) gcgcCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGc < 7:170778/149‑1 (MQ=255) cgcCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGG‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAATGCCCCACAGCGGCTGTCGCTGGAAGGCTTGc < 1:38458/149‑1 (MQ=255) cgcCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGATCCCATTACCGTGCAA‑GCCTTCCAGCGACAGCCGCTGTGGGGCTTGCa < 6:385403/149‑1 (MQ=38) cgcCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCTTCCAGCGACAGCCGCTGTGGGGCTTGCa < 7:127964/149‑1 (MQ=38) gcCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCAt < 4:356452/149‑1 (MQ=255) ccTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCAt > 1:168843/1‑148 (MQ=255) ttGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCTTCCAGCGACAGCCGCTGTGGGGCTTGCacggt < 8:121578/147‑5 (MQ=37) ttGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGAc < 3:56994/147‑1 (MQ=255) cTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACGGCGGCTGTCGCTGGAAGTCATGCAAGCCTTCCa < 4:26010/149‑1 (MQ=255) cTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCa < 4:174303/149‑1 (MQ=255) aCCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAgg < 7:14216/148‑1 (MQ=255) aCCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGc < 1:56972/149‑1 (MQ=255) ccGGCCTGCCGAGGACTGCGCGGCGCATGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGc > 1:193736/1‑135 (MQ=255) ccGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGc < 2:193736/134‑1 (MQ=255) cGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGAt > 5:198025/1‑149 (MQ=255) cGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGAt > 1:35914/1‑149 (MQ=255) ggCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATc < 3:303484/149‑1 (MQ=255) cTGCCGAGGACTGCGCAGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGc < 5:276530/142‑1 (MQ=255) ccGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATtgtg < 1:22008/149‑1 (MQ=255) ccGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATtgt < 5:94750/148‑1 (MQ=255) ccGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATtgt > 6:94750/1‑148 (MQ=255) gAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGt < 3:84296/149‑1 (MQ=255) aGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTc < 3:248597/149‑1 (MQ=255) tGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACt > 2:66186/1‑124 (MQ=255) tGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACt < 1:66186/124‑1 (MQ=255) tGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTc < 7:38146/144‑1 (MQ=255) tGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTc > 8:38146/1‑144 (MQ=255) gcggcgC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCGCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCCCAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAgcc > 1:268185/1‑149 (MQ=255) gcggcgC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAgcc > 7:182100/1‑149 (MQ=255) gc‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAgccgggcg > 6:193338/1‑149 (MQ=255) gc‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAgccggccg > 4:113867/1‑149 (MQ=255) gc‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGAc < 4:222396/109‑1 (MQ=255) gc‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGAc > 3:222396/1‑109 (MQ=255) aaaGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCGCTGCTGGAGATTGAACCCATTACCGTGCAC‑GCCCCCCAGCGGCTGTCGCTGGAAGGGTTGCATGACTTCCCGGCGATCATGGTGGTCAGCAAGCCGGCCGCCCGGcgg > 8:194336/1‑147 (MQ=255) aGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGtt > 8:74646/1‑149 (MQ=255) cTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCCTTCCCGTGCAA‑GCCCCCCCGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGGCAGCAAGCCGGGCGGCCGGCttgctga < 2:347316/149‑7 (MQ=255) cTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGGCGGCCGGCttgctga < 3:200806/149‑7 (MQ=255) cTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCt > 4:362212/1‑149 (MQ=255) gcggcAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGa < 6:198025/149‑1 (MQ=255) gcggcAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGa < 2:168843/149‑1 (MQ=255) aGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATc > 2:4211/1‑149 (MQ=255) gCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCtt < 5:64113/141‑1 (MQ=255) gCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCtt > 6:64113/1‑141 (MQ=255) gAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGcc > 2:136408/1‑149 (MQ=255) gAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGcc < 7:89204/149‑1 (MQ=255) gggCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCgaga > 3:103565/1‑149 (MQ=255) gcagcaGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCt < 8:250423/130‑1 (MQ=255) gcagcaGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCt > 7:250423/1‑130 (MQ=255) gcagcaGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGAc > 2:64697/1‑148 (MQ=255) cagcagTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCGGTTGCTTGAGCATCTGGCCGAGAc > 6:48555/1‑147 (MQ=255) cagTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑Ca > 6:202357/1‑149 (MQ=255) gcctgccCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCa < 6:989/148‑1 (MQ=255) cctgccCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGGCGGCCGGCttgc > 5:11722/1‑114 (MQ=255) cctgccCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGGCGGCCGGCttgc < 6:11722/117‑4 (MQ=255) gacccTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTc > 1:91203/3‑149 (MQ=255) ccccTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTcc > 7:8233/1‑149 (MQ=255) ctgctgGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCa > 8:294561/1‑149 (MQ=255) gagaTTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGa < 5:202357/147‑1 (MQ=255) gagaTTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGa < 1:64697/147‑1 (MQ=255) gagaTTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAgg < 1:247100/149‑1 (MQ=255) gagaTTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAgg > 2:184194/1‑149 (MQ=255) agaTTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGc < 8:182100/149‑1 (MQ=255) gaTTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCt > 4:206121/1‑149 (MQ=255) ttGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTgg < 7:303382/149‑1 (MQ=255) tGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGt > 2:29479/1‑149 (MQ=255) gAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGtt > 6:61674/1‑149 (MQ=255) aaCCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAGCCATCCAGCCTCCGCAGCGAGGCTGGtt < 1:4211/149‑1 (MQ=255) aaCCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGtt > 6:277388/1‑148 (MQ=255) cccATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCAc < 2:283912/149‑1 (MQ=255) ccATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTAGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCAcc > 1:49372/1‑149 (MQ=255) cATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCg < 3:113867/149‑1 (MQ=255) cATTACCATGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAgccgg > 1:374131/1‑80 (MQ=255) cATTACCATGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAgccgg < 2:374131/80‑1 (MQ=255) aTTACCGTGCAA‑GCCACACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGACGGCCGACCGGCTGTTGCTTGAGAATCTCGCAGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGt > 8:353903/1‑149 (MQ=255) aCCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTgg < 7:74646/148‑1 (MQ=255) aCCGTGCAA‑GCCCCACAGCGGCTGTAGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGTTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGAAGCGAGGCTGGTTCACCGTggg > 7:282643/1‑149 (MQ=255) gTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGa < 1:136408/149‑1 (MQ=255) tGCAA‑GCCTTCCAGCGACAGCCGCTGTGGGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGAg > 1:220823/1‑149 (MQ=38) gCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGAgg > 3:248350/1‑149 (MQ=255) cAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGAgg > 7:265571/1‑148 (MQ=255) cAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGAgg > 4:161915/1‑148 (MQ=255) ccccACAGCGGCTGTCGCTGGCAGGCTTGCAAGACTGCCAGGCGATCATTCAGGTCAGCAAGACGGCCGCCCGGCTGTTGCTAGAGCATCTGGCCGAGCCCAG‑CCTCCGGCCGCCCCAGCGAGGCGCGTACCCCGGGGGAGAGGCAccc > 6:213271/1‑149 (MQ=255) cccACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGAGGCAACCg > 5:297915/1‑149 (MQ=255) ccACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGAGGCAACCGc > 3:276782/1‑149 (MQ=255) ccACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGAGGCAACCGc > 5:317736/1‑149 (MQ=255) ccACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGAGGCAACCGc < 8:8233/149‑1 (MQ=255) | GCGAGGCCGGTCACCCGTGAGCCAATGGCGCCTGTTGCTGACCCGGCCTGCCGAGGACTGCGCGGCGC‑TGGCGC‑AAAGCCTGGCGGCAGCGGGAGTGGGCAGCAGTTGCCTGCCCCTGCTGGAGATTGAACCCATTACCGTGCAA‑GCCCCACAGCGGCTGTCGCTGGAAGGCTTGCATGACTTCCAGGCGATCATTGTGGTCAGCAAGCCGGCCGCCCGGCTGTTGCTTGAGCATCTGGCCGAGACCAG‑CATCCAGCCTCCGCAGCGAGGCTGGTTCACCGTGGGTGAGGCAACCGC > NC_002947/238904‑239200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |