Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,177,809 T→C 12.2% intergenic (‑47/+117) PP_2788 ← / ← PP_2789 MerR family transcriptional regulator/oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,177,8090TC12.2% 125.8 / 7.6 49intergenic (‑47/+117)PP_2788/PP_2789MerR family transcriptional regulator/oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (25/18);  new base C (3/3);  total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTGCCTTCGCGTTGCGGTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGACTTGTTT  >  NC_002947/3177662‑3177955
                                                                                                                                                   |                                                                                                                                                  
ttGCCTTCGCGTTGCGGTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTc                                                                                                                                                   >  4:13586/1‑149 (MQ=255)
             gCGGTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGgcgcgc                                                                                                                                       <  3:13586/148‑1 (MQ=255)
               ggTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGCGCCTGTACGAGCACGCGAc                                                                                                                                    <  1:75848/149‑1 (MQ=255)
                gTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACa                                                                                                                                   >  4:152967/1‑149 (MQ=255)
                   ggggTAGCAGGCCGATTTCTTCGTACTGGCGGATGCTTTTGACGGTGGTGCCCGCCAGCTGGGCGGCTTTGCCGATGGACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACAttt                                                                                                                                <  1:141060/149‑1 (MQ=255)
                    gggTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACAtttt                                                                                                                               >  1:79519/1‑149 (MQ=255)
                     ggTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACAttttt                                                                                                                              <  4:348644/149‑1 (MQ=255)
                         gCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGca                                                                                                                           >  4:370855/1‑148 (MQ=255)
                           agaccGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGCGCCTGTACGAGCACGCGACAtc                                                                                                                                 >  4:133641/4‑139 (MQ=255)
                           agaccGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGCGCCTGTACGAGCACGCGACAtc                                                                                                                                 <  3:139236/137‑2 (MQ=255)
                           agaccGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGCGCCTGTACGAGCACGCGACAtc                                                                                                                                 <  3:133641/137‑2 (MQ=255)
                           agaccGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGCGCCTGTACGAGCACGCGACAtc                                                                                                                                 >  4:139236/4‑139 (MQ=255)
                               cGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCAGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGt                                                                                                                    >  8:123337/1‑149 (MQ=255)
                                           aaTGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTggg                                                                                                          >  4:295002/1‑147 (MQ=255)
                                           aaTGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATCTTTGCACAAGCGTCAGGGGTggg                                                                                                          >  2:263258/1‑147 (MQ=255)
                                            aTGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGaa                                                                                                        <  3:152967/148‑1 (MQ=255)
                                            aTGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGAGAAAAGATGTCCCGTTCTCGTACAGGCGCGAGACTTTTTTGCAAAAGCGTCAGGGGTGGGAAg                                                                                                       >  4:247239/1‑149 (MQ=255)
                                                 ggATGCTTTTGACTGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAg                                                                                                  <  6:328320/149‑1 (MQ=255)
                                                   aTGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCt                                                                                                >  6:280450/1‑149 (MQ=255)
                                                       ttttGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGaa                                                                                             >  7:327463/1‑148 (MQ=255)
                                                         ttGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGaaaa                                                                                           <  2:79519/148‑1 (MQ=255)
                                                          tGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAg                                                                                          <  4:57815/148‑1 (MQ=255)
                                                             cGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCTATTAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGt                                                                                      <  3:309864/149‑1 (MQ=255)
                                                              ggtggtGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTTACGCATCAGAAGCGAAAAGATGTAGCGTGCTCGTACAGGCGCGCGACAATTATGCACAACCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGt                                                                                      >  7:170196/1‑148 (MQ=255)
                                                                   tgCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAg                                                                                 >  3:33090/1‑148 (MQ=255)
                                                                     cccGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCAc                                                                              >  2:109747/1‑149 (MQ=255)
                                                                          cAGCTGGGCGGCTTTGACGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCTTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGTGAAGCCAAGCTGAAAAGGCGTCACAGCAcggcgg                                                                         <  3:247239/149‑1 (MQ=255)
                                                                            gCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGcggct                                                                       <  3:295002/149‑1 (MQ=255)
                                                                               gggCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTAGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACCTCACGGcggctcgg                                                                    >  8:171613/1‑149 (MQ=255)
                                                                                 gcagcTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGcggctcggct                                                                  <  7:123337/146‑1 (MQ=255)
                                                                                        tGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATCTTTGCACAAGCGTCAGGGGTGGCAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCa                                                             <  1:263258/147‑1 (MQ=255)
                                                                                         gCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGACCGGCGGCTCGGCTGGGCAgcg                                                          >  3:225236/1‑149 (MQ=255)
                                                                                                    tGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACgg                                               >  6:44811/1‑149 (MQ=255)
                                                                                                     gAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGc                                              >  1:360532/1‑149 (MQ=255)
                                                                                                      aaaaTCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGcc                                             >  8:172548/1‑149 (MQ=255)
                                                                                                          tCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGAc                                         <  4:33090/149‑1 (MQ=255)
                                                                                                             cTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCgg                                      >  4:95932/1‑149 (MQ=255)
                                                                                                             cTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCgg                                      <  1:117554/149‑1 (MQ=255)
                                                                                                              ttttttGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCg                                           >  1:104680/1‑143 (MQ=255)
                                                                                                              ttttttGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCg                                           <  2:104680/143‑1 (MQ=255)
                                                                                                                ttttGACGCATGAGAAGCGAAAAGATGTCGCGTGGTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCggg                                     <  8:159947/147‑1 (MQ=255)
                                                                                                                       gCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCAGACCGGGGCCGATccg                            >  6:261455/1‑147 (MQ=255)
                                                                                                                                gCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTg                      <  8:195241/146‑1 (MQ=255)
                                                                                                                                gCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTg                      >  7:195241/1‑146 (MQ=255)
                                                                                                                                gCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTCTTAAGGCGTCACAGCAAGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGc                    >  1:117135/1‑148 (MQ=255)
                                                                                                                                   aaaaGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACt                >  5:361382/1‑149 (MQ=255)
                                                                                                                                          gTCGCGCGCCTGTACGAGCACGCGACATCTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGa         >  8:182917/1‑149 (MQ=255)
                                                                                                                                           tCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGAc        >  4:265026/1‑149 (MQ=255)
                                                                                                                                           tCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGAc        <  3:265026/149‑1 (MQ=255)
                                                                                                                                                   tCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGACTTGttt  >  3:283979/1‑147 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGCCTTCGCGTTGCGGTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGACTTGTTT  >  NC_002947/3177662‑3177955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑