Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,177,815 A→G 12.0% intergenic (‑53/+111) PP_2788 ← / ← PP_2789 MerR family transcriptional regulator/oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,177,8150AG12.0% 125.8 / 8.3 50intergenic (‑53/+111)PP_2788/PP_2789MerR family transcriptional regulator/oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (25/19);  new base G (3/3);  total (28/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

GCGGTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGACTTGTTTGAGCCAGG  >  NC_002947/3177675‑3177963
                                                                                                                                            |                                                                                                                                                    
gCGGTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGgcgcgc                                                                                                                                               <  3:13586/148‑1 (MQ=255)
  ggTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGCGCCTGTACGAGCACGCGAc                                                                                                                                            <  1:75848/149‑1 (MQ=255)
   gTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACa                                                                                                                                           >  4:152967/1‑149 (MQ=255)
      ggggTAGCAGGCCGATTTCTTCGTACTGGCGGATGCTTTTGACGGTGGTGCCCGCCAGCTGGGCGGCTTTGCCGATGGACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACAttt                                                                                                                                        <  1:141060/149‑1 (MQ=255)
       gggTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACAtttt                                                                                                                                       >  1:79519/1‑149 (MQ=255)
        ggTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACAttttt                                                                                                                                      <  4:348644/149‑1 (MQ=255)
            gCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGca                                                                                                                                   >  4:370855/1‑148 (MQ=255)
              agaccGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGCGCCTGTACGAGCACGCGACAtc                                                                                                                                         >  4:139236/4‑139 (MQ=255)
              agaccGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGCGCCTGTACGAGCACGCGACAtc                                                                                                                                         >  4:133641/4‑139 (MQ=255)
              agaccGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGCGCCTGTACGAGCACGCGACAtc                                                                                                                                         <  3:139236/137‑2 (MQ=255)
              agaccGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGCGCCTGTACGAGCACGCGACAtc                                                                                                                                         <  3:133641/137‑2 (MQ=255)
                  cGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCAGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGt                                                                                                                            >  8:123337/1‑149 (MQ=255)
                              aaTGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTggg                                                                                                                  >  4:295002/1‑147 (MQ=255)
                              aaTGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATCTTTGCACAAGCGTCAGGGGTggg                                                                                                                  >  2:263258/1‑147 (MQ=255)
                               aTGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGaa                                                                                                                <  3:152967/148‑1 (MQ=255)
                               aTGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGAGAAAAGATGTCCCGTTCTCGTACAGGCGCGAGACTTTTTTGCAAAAGCGTCAGGGGTGGGAAg                                                                                                               >  4:247239/1‑149 (MQ=255)
                                    ggATGCTTTTGACTGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAg                                                                                                          <  6:328320/149‑1 (MQ=255)
                                      aTGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCt                                                                                                        >  6:280450/1‑149 (MQ=255)
                                          ttttGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGaa                                                                                                     >  7:327463/1‑148 (MQ=255)
                                            ttGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGaaaa                                                                                                   <  2:79519/148‑1 (MQ=255)
                                             tGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAg                                                                                                  <  4:57815/148‑1 (MQ=255)
                                                cGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCTATTAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGt                                                                                              <  3:309864/149‑1 (MQ=255)
                                                 ggtggtGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTTACGCATCAGAAGCGAAAAGATGTAGCGTGCTCGTACAGGCGCGCGACAATTATGCACAACCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGt                                                                                              >  7:170196/1‑148 (MQ=255)
                                                      tgCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAg                                                                                         >  3:33090/1‑148 (MQ=255)
                                                        cccGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCAc                                                                                      >  2:109747/1‑149 (MQ=255)
                                                             cAGCTGGGCGGCTTTGACGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCTTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGTGAAGCCAAGCTGAAAAGGCGTCACAGCAcggcgg                                                                                 <  3:247239/149‑1 (MQ=255)
                                                               gCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGcggct                                                                               <  3:295002/149‑1 (MQ=255)
                                                                  gggCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTAGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACCTCACGGcggctcgg                                                                            >  8:171613/1‑149 (MQ=255)
                                                                    gcagcTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGcggctcggct                                                                          <  7:123337/146‑1 (MQ=255)
                                                                           tGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATCTTTGCACAAGCGTCAGGGGTGGCAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCa                                                                     <  1:263258/147‑1 (MQ=255)
                                                                            gCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGACCGGCGGCTCGGCTGGGCAgcg                                                                  >  3:225236/1‑149 (MQ=255)
                                                                                       tGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACgg                                                       >  6:44811/1‑149 (MQ=255)
                                                                                        gAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGc                                                      >  1:360532/1‑149 (MQ=255)
                                                                                         aaaaTCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGcc                                                     >  8:172548/1‑149 (MQ=255)
                                                                                             tCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGAc                                                 <  4:33090/149‑1 (MQ=255)
                                                                                                cTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCgg                                              >  4:95932/1‑149 (MQ=255)
                                                                                                cTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCgg                                              <  1:117554/149‑1 (MQ=255)
                                                                                                 ttttttGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCg                                                   <  2:104680/143‑1 (MQ=255)
                                                                                                 ttttttGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCg                                                   >  1:104680/1‑143 (MQ=255)
                                                                                                   ttttGACGCATGAGAAGCGAAAAGATGTCGCGTGGTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCggg                                             <  8:159947/147‑1 (MQ=255)
                                                                                                          gCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCAGACCGGGGCCGATccg                                    >  6:261455/1‑147 (MQ=255)
                                                                                                                   gCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTg                              >  7:195241/1‑146 (MQ=255)
                                                                                                                   gCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTg                              <  8:195241/146‑1 (MQ=255)
                                                                                                                   gCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTCTTAAGGCGTCACAGCAAGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGc                            >  1:117135/1‑148 (MQ=255)
                                                                                                                      aaaaGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACt                        >  5:361382/1‑149 (MQ=255)
                                                                                                                             gTCGCGCGCCTGTACGAGCACGCGACATCTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGa                 >  8:182917/1‑149 (MQ=255)
                                                                                                                              tCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGAc                >  4:265026/1‑149 (MQ=255)
                                                                                                                              tCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGAc                <  3:265026/149‑1 (MQ=255)
                                                                                                                                      tCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGACTTGttt          >  3:283979/1‑147 (MQ=255)
                                                                                                                                        gTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGACTTGTTTGa        <  6:361382/147‑1 (MQ=255)
                                                                                                                                            aGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTg                   >  4:251995/1‑132 (MQ=255)
                                                                                                                                            aGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTg                   <  3:251995/132‑1 (MQ=255)
                                                                                                                                            aGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGACTTGTTTGAGCCAgg  >  7:171276/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
GCGGTGGGGGTAGCAGGCCGATTTCTTCGTAATGGCGGATGCTTTTGACGGTGGTGCCCGACAGCTGGGCGGCTTTGCCGATGTACATGAAAATCCCTTTTTTGACGCATCAGAAGCGAAAAGATGTCGCGTGCTCGTACAGGCGCGCGACATTTTTGCACAAGCGTCAGGGGTGGGAAGCCAAGCTGAAAAGGCGTCACAGCACGGCGGCTCGGCTGGGCAGCGCAGGGGCACGGCCCGACCGGGGCCGATCAGCGGCTGGCGACTGCCTGGACTTGTTTGAGCCAGG  >  NC_002947/3177675‑3177963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑