Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,267,960 A→G 6.4% H144R (CAC→CGC) ‡ azoR1 → FMN‑dependent NADH‑azoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,267,9600AG 6.4% 187.0 / 2.3 63H144R (CAC→CGC) ‡azoR1FMN‑dependent NADH‑azoreductase
Reads supporting (aligned to +/- strand):  ref base A (31/27);  new base G (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

TACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCG  >  NC_002947/3267815‑3268108
                                                                                                                                                 |                                                                                                                                                    
tACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGc                                                                                                                                                   >  5:25158/1‑149 (MQ=255)
 aCAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCt                                                                                                                                                  >  8:251372/1‑149 (MQ=255)
 aCAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCt                                                                                                                                                  >  6:64570/1‑149 (MQ=255)
  cAACTTCACCGTGCCGACCCAGCTCATAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTg                                                                                                                                                 <  4:71778/149‑1 (MQ=255)
   aaCTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTgg                                                                                                                                                >  1:203238/1‑149 (MQ=255)
   aaCTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTgg                                                                                                                                                >  6:44032/1‑149 (MQ=255)
      ttCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGc                                                                                                                                               >  8:239206/1‑147 (MQ=255)
        cACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAg                                                                                                                                            <  3:310945/148‑1 (MQ=255)
        cACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGc                                                                                                                                           >  6:89460/1‑149 (MQ=255)
         aCCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGcc                                                                                                                                          <  1:146144/149‑1 (MQ=255)
               ccGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTgg                                                                                                                                    <  8:320616/149‑1 (MQ=255)
               ccGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGCTGGCCAGCGTGCAGGCCACCCgcagtg                                                                                                                                    <  6:27552/149‑6 (MQ=255)
                 gACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGCGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGgcg                                                                                                                                  >  5:220235/1‑149 (MQ=255)
                    ccAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCg                                                                                                                               >  7:255911/1‑149 (MQ=255)
                    ccAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCg                                                                                                                               >  6:209335/1‑149 (MQ=255)
                    ccAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCg                                                                                                                               <  2:162854/149‑1 (MQ=255)
                          cAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCAGCGTGCAGGCCac                                                                                                                                            >  5:103969/1‑129 (MQ=255)
                          cAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCAGCGTGCAGGCCac                                                                                                                                            <  6:103969/130‑2 (MQ=255)
                           aaaGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATg                                                                                                                         >  8:139734/1‑148 (MQ=255)
                           aaaGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATg                                                                                                                         >  5:35388/1‑148 (MQ=255)
                            aaGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGaa                                                                                                                       <  1:337784/149‑1 (MQ=255)
                               ccTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGAc                                                                                                                    >  5:346238/1‑149 (MQ=255)
                                   gATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCTAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACtt                                                                                                                  <  6:66674/147‑1 (MQ=255)
                                     ttGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACt                                                                                                                                      >  2:112980/1‑125 (MQ=255)
                                     ttGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACt                                                                                                                                      <  1:112980/125‑1 (MQ=255)
                                     ttGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCt                                                                                                               >  4:72404/1‑148 (MQ=255)
                                        aCCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGaaa                                                                                                           <  6:35388/149‑1 (MQ=255)
                                        aCCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGa                                                                                                             >  4:174416/1‑147 (MQ=255)
                                        aCCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGa                                                                                                             >  1:249156/1‑147 (MQ=255)
                                           gcgTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAgtg                                                                                                        <  5:89460/149‑1 (MQ=255)
                                                  cGTCGCCGGCAAGACCTACCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGCTGCACGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGCCATGAAGACTACCTGAAAGTGTTCCTgg                                                                                                 >  5:46334/1‑149 (MQ=255)
                                                             aGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCggg                                                                                      <  7:251372/149‑1 (MQ=255)
                                                              gACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGa                                                                                     <  3:72404/149‑1 (MQ=255)
                                                                 cTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTa                                                                                  >  3:230633/1‑149 (MQ=255)
                                                                   tCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACt                                                                                >  5:26432/1‑149 (MQ=255)
                                                                    ccGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACt                                                                                <  6:26432/148‑1 (MQ=255)
                                                                          cacCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTgg                                                                         <  5:209335/149‑1 (MQ=255)
                                                                          cacCGAAGCCGGCCCGCAAGGCCTGCGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCt                                                                           <  6:220235/147‑1 (MQ=255)
                                                                             cGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCGGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGAGCCGGGCTTGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGaa                                                                       >  5:363903/1‑148 (MQ=255)
                                                                             cGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGGGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCATGAAAGTGTTGCTGGGCTTCATCGGGATTACTGACCTGGaaa                                                                      >  7:254823/1‑149 (MQ=255)
                                                                              gAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAAt                                                                     >  7:286906/1‑149 (MQ=255)
                                                                               aaGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATc                                                                    <  8:262668/149‑1 (MQ=255)
                                                                                  ccGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTc                                                                 >  8:269424/1‑149 (MQ=255)
                                                                                        cGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGcc                                                           <  6:346238/149‑1 (MQ=255)
                                                                                          cAAGCCCTGGGCGGCAACAAGAAAGCGGGGCTGGGTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCa                                                         <  8:255911/149‑1 (MQ=255)
                                                                                                cTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACggcct                                                   <  4:230633/149‑1 (MQ=255)
                                                                                                    gCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACggcctggcc                                               <  5:64570/149‑1 (MQ=255)
                                                                                                         aacaaGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATgg                                          >  8:61409/1‑149 (MQ=255)
                                                                                                             aGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATTAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCg                                      <  7:239206/149‑1 (MQ=255)
                                                                                                               aaaGTGGTGCTGGTTTCCACTGCGGGGGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCATAGGCCAGGCCGTGGGCCCGGa                                    <  2:192430/149‑1 (MQ=255)
                                                                                                                  gtggtgCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGagca                                 <  6:25158/149‑1 (MQ=255)
                                                                                                                  gtggtgCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGagca                                 <  2:203238/149‑1 (MQ=255)
                                                                                                                      tgCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAgcagcg                              >  7:135141/1‑148 (MQ=255)
                                                                                                                       gCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTCGCCTATGGCCCGGAgcagcg                              >  4:352773/1‑147 (MQ=255)
                                                                                                                            tttCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGGTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAg                       <  5:44032/149‑1 (MQ=255)
                                                                                                                                 aCTGCTGGTGGCCTGCCCTCTGGCCAGCCGACTGGCGCCGGGCATGACGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTTGAAATCGTCCGTGCCCACTGCCTGGCCTATGGCCCGGAGCAGCGCAGCCACGcgat                  <  6:363903/149‑1 (MQ=255)
                                                                                                                                 aCTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgat                  >  4:33545/1‑149 (MQ=255)
                                                                                                                                   tGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgatc                 >  2:198225/1‑148 (MQ=255)
                                                                                                                                     cGGGTGGCCTGCACGCTGGCGAGCCTACTGGCGGAGTGCATGAAGCCTTCCTGATAGTGGTCCTGGTCTTCATCGGGTTTTCTGACCTGGAAATCGTCCGGGCCCACGGGCTGGCCTATGGCGCGGAGGTGCGCAGCCAGGcgatcgat              <  6:46334/149‑1 (MQ=255)
                                                                                                                                        ctGGCCAGCGTGCAGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGAtgct           >  2:4750/2‑149 (MQ=255)
                                                                                                                                             cTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGca        >  1:161729/1‑147 (MQ=255)
                                                                                                                                                cACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgatcg                >  5:322159/1‑136 (MQ=255)
                                                                                                                                                cACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgatcg                <  6:322159/136‑1 (MQ=255)
                                                                                                                                                 aCGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCTGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgcg  >  1:322740/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCG  >  NC_002947/3267815‑3268108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑