Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,267,964 T→A 6.3% A145A (GCT→GCA)  azoR1 → FMN‑dependent NADH‑azoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,267,9640TA 6.3% 183.8 / 2.4 63A145A (GCT→GCA) azoR1FMN‑dependent NADH‑azoreductase
Reads supporting (aligned to +/- strand):  ref base T (31/28);  new base A (2/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

ACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCG  >  NC_002947/3267816‑3268108
                                                                                                                                                    |                                                                                                                                                
aCAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCt                                                                                                                                                  >  6:64570/1‑149 (MQ=255)
aCAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCt                                                                                                                                                  >  8:251372/1‑149 (MQ=255)
 cAACTTCACCGTGCCGACCCAGCTCATAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTg                                                                                                                                                 <  4:71778/149‑1 (MQ=255)
  aaCTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTgg                                                                                                                                                >  6:44032/1‑149 (MQ=255)
  aaCTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTgg                                                                                                                                                >  1:203238/1‑149 (MQ=255)
     ttCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGc                                                                                                                                               >  8:239206/1‑147 (MQ=255)
       cACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAg                                                                                                                                            <  3:310945/148‑1 (MQ=255)
       cACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGc                                                                                                                                           >  6:89460/1‑149 (MQ=255)
        aCCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGcc                                                                                                                                          <  1:146144/149‑1 (MQ=255)
              ccGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTgg                                                                                                                                    <  8:320616/149‑1 (MQ=255)
              ccGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGCTGGCCAGCGTGCAGGCCACCCgcagtg                                                                                                                                    <  6:27552/149‑6 (MQ=255)
                gACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGCGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGgcg                                                                                                                                  >  5:220235/1‑149 (MQ=255)
                   ccAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCg                                                                                                                               <  2:162854/149‑1 (MQ=255)
                   ccAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCg                                                                                                                               >  6:209335/1‑149 (MQ=255)
                   ccAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCg                                                                                                                               >  7:255911/1‑149 (MQ=255)
                         cAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCAGCGTGCAGGCCac                                                                                                                                            <  6:103969/130‑2 (MQ=255)
                         cAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCAGCGTGCAGGCCac                                                                                                                                            >  5:103969/1‑129 (MQ=255)
                          aaaGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATg                                                                                                                         >  5:35388/1‑148 (MQ=255)
                          aaaGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATg                                                                                                                         >  8:139734/1‑148 (MQ=255)
                           aaGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGaa                                                                                                                       <  1:337784/149‑1 (MQ=255)
                              ccTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGAc                                                                                                                    >  5:346238/1‑149 (MQ=255)
                                  gATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCTAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACtt                                                                                                                  <  6:66674/147‑1 (MQ=255)
                                    ttGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACt                                                                                                                                      >  2:112980/1‑125 (MQ=255)
                                    ttGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACt                                                                                                                                      <  1:112980/125‑1 (MQ=255)
                                    ttGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCt                                                                                                               >  4:72404/1‑148 (MQ=255)
                                       aCCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGaaa                                                                                                           <  6:35388/149‑1 (MQ=255)
                                       aCCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGa                                                                                                             >  1:249156/1‑147 (MQ=255)
                                       aCCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGa                                                                                                             >  4:174416/1‑147 (MQ=255)
                                          gcgTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAgtg                                                                                                        <  5:89460/149‑1 (MQ=255)
                                                 cGTCGCCGGCAAGACCTACCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGCTGCACGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGCCATGAAGACTACCTGAAAGTGTTCCTgg                                                                                                 >  5:46334/1‑149 (MQ=255)
                                                            aGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCggg                                                                                      <  7:251372/149‑1 (MQ=255)
                                                             gACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGa                                                                                     <  3:72404/149‑1 (MQ=255)
                                                                cTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTa                                                                                  >  3:230633/1‑149 (MQ=255)
                                                                  tCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACt                                                                                >  5:26432/1‑149 (MQ=255)
                                                                   ccGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACt                                                                                <  6:26432/148‑1 (MQ=255)
                                                                         cacCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTgg                                                                         <  5:209335/149‑1 (MQ=255)
                                                                         cacCGAAGCCGGCCCGCAAGGCCTGCGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCt                                                                           <  6:220235/147‑1 (MQ=255)
                                                                            cGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCGGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGAGCCGGGCTTGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGaa                                                                       >  5:363903/1‑148 (MQ=255)
                                                                            cGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGGGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCATGAAAGTGTTGCTGGGCTTCATCGGGATTACTGACCTGGaaa                                                                      >  7:254823/1‑149 (MQ=255)
                                                                             gAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAAt                                                                     >  7:286906/1‑149 (MQ=255)
                                                                              aaGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATc                                                                    <  8:262668/149‑1 (MQ=255)
                                                                                 ccGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTc                                                                 >  8:269424/1‑149 (MQ=255)
                                                                                       cGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGcc                                                           <  6:346238/149‑1 (MQ=255)
                                                                                         cAAGCCCTGGGCGGCAACAAGAAAGCGGGGCTGGGTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCa                                                         <  8:255911/149‑1 (MQ=255)
                                                                                               cTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACggcct                                                   <  4:230633/149‑1 (MQ=255)
                                                                                                   gCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACggcctggcc                                               <  5:64570/149‑1 (MQ=255)
                                                                                                        aacaaGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATgg                                          >  8:61409/1‑149 (MQ=255)
                                                                                                            aGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATTAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCg                                      <  7:239206/149‑1 (MQ=255)
                                                                                                              aaaGTGGTGCTGGTTTCCACTGCGGGGGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCATAGGCCAGGCCGTGGGCCCGGa                                    <  2:192430/149‑1 (MQ=255)
                                                                                                                 gtggtgCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGagca                                 <  6:25158/149‑1 (MQ=255)
                                                                                                                 gtggtgCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGagca                                 <  2:203238/149‑1 (MQ=255)
                                                                                                                     tgCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAgcagcg                              >  7:135141/1‑148 (MQ=255)
                                                                                                                      gCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTCGCCTATGGCCCGGAgcagcg                              >  4:352773/1‑147 (MQ=255)
                                                                                                                           tttCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGGTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAg                       <  5:44032/149‑1 (MQ=255)
                                                                                                                                aCTGCTGGTGGCCTGCCCTCTGGCCAGCCGACTGGCGCCGGGCATGACGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTTGAAATCGTCCGTGCCCACTGCCTGGCCTATGGCCCGGAGCAGCGCAGCCACGcgat                  <  6:363903/149‑1 (MQ=255)
                                                                                                                                aCTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgat                  >  4:33545/1‑149 (MQ=255)
                                                                                                                                  tGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgatc                 >  2:198225/1‑148 (MQ=255)
                                                                                                                                    cGGGTGGCCTGCACGCTGGCGAGCCTACTGGCGGAGTGCATGAAGCCTTCCTGATAGTGGTCCTGGTCTTCATCGGGTTTTCTGACCTGGAAATCGTCCGGGCCCACGGGCTGGCCTATGGCGCGGAGGTGCGCAGCCAGGcgatcgat              <  6:46334/149‑1 (MQ=255)
                                                                                                                                       ctGGCCAGCGTGCAGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGAtgct           >  2:4750/2‑149 (MQ=255)
                                                                                                                                            cTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGca        >  1:161729/1‑147 (MQ=255)
                                                                                                                                               cACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgatcg                >  5:322159/1‑136 (MQ=255)
                                                                                                                                               cACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGcgatcg                <  6:322159/136‑1 (MQ=255)
                                                                                                                                                aCGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCTGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgcg  >  1:322740/1‑149 (MQ=255)
                                                                                                                                                  gCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGgcg  >  6:338604/1‑147 (MQ=255)
                                                                                                                                                  gCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTACCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACCGgcg  >  5:97916/1‑147 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ACAACTTCACCGTGCCGACCCAGCTCAAAGCCTGGATTGACCGCGTAGCCGTCGCCGGCAAGACCTTCCGCTACACCGAAGCCGGCCCGCAAGGCCTGTGCGGCAACAAGAAAGTGGTGCTGGTTTCCACTGCGGGTGGCCTGCACGCTGGCCAGCCGACTGGCGCCGGGCATGAAGACTTCCTGAAAGTGTTCCTGGGCTTCATCGGGATTACTGACCTGGAAATCGTCCGGGCCCACGGCCTGGCCTATGGCCCGGAGCAGCGCAGCCAGGCGATCGATGCTGCACAGGCG  >  NC_002947/3267816‑3268108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑