Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,304,630	G→T	7.7%	L422L (CTG→CTT)	cysS →	cysteine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,304,630	0	G	T	7.7%	205.8 / 4.8	65	L422L (CTG→CTT)	cysS	cysteine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (28/32);  new base T (2/3);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GGAGGCCGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGC  >  NC_002947/3304483‑3304776                                                                                                                                                     |                                                                                                                                                   ggAGGCCGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTg                                                                                                                                                    >  5:35481/1‑148 (MQ=255)   aGGCCGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAg                                                                                                                                                 <  3:63074/149‑1 (MQ=255)       cGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGgcgc                                                                                                                                             >  7:229094/1‑149 (MQ=255)       cGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGgcgc                                                                                                                                             <  6:26625/149‑1 (MQ=255)       cGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGgcgc                                                                                                                                             >  3:61295/1‑149 (MQ=255)        gctgctGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGcgcg                                                                                                                                            >  2:239759/1‑149 (MQ=255)         ctgctgGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTTCAGGcgcgc                                                                                                                                           <  6:176881/149‑2 (MQ=255)          tgctgGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTTCAGGcgcgcc                                                                                                                                          >  6:235866/1‑147 (MQ=255)          tgctgGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTTCAGGcgcgcc                                                                                                                                          <  5:241282/149‑3 (MQ=255)          tgctgGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCGTCAGGcgcgcc                                                                                                                                          >  6:241282/1‑147 (MQ=255)            ctggcctggcCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTTCAGGcgcgcc                                                                                                                                          <  5:235866/147‑3 (MQ=255)            ctggcctggcCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCg                                                                                                                                        >  1:234793/1‑149 (MQ=255)            ctggcctggcCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCg                                                                                                                                        >  2:98755/1‑149 (MQ=255)             tggactggccGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCg                                                                                                                                        >  1:368481/1‑148 (MQ=255)                 ctggccGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACaa                                                                                                                                   <  8:229094/149‑1 (MQ=255)                    gccGGTCGACTGCGCGAGCTGGGTGATGTACTGGGATGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCGTGAAGGCGCGTGCGGACAAg                                                                                                                                  >  1:55015/1‑148 (MQ=255)                    gccGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAg                                                                                                                                  <  2:234793/147‑1 (MQ=255)                       ggTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTg                                                                                                                             >  7:276370/1‑149 (MQ=255)                         tCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGGGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTggg                                                                                                                           <  1:365900/149‑1 (MQ=255)                           gACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGc                                                                                                                          <  7:61150/148‑1 (MQ=255)                           gACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCt                                                                                                                         <  2:18701/149‑1 (MQ=255)                             cTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGa                                                                                                                       <  8:39604/149‑1 (MQ=255)                              tGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAg                                                                                                                      >  7:196384/1‑149 (MQ=255)                                   gAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGa                                                                                                                 <  8:52924/149‑1 (MQ=255)                                   gAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGa                                                                                                                 <  2:98868/149‑1 (MQ=255)                                     gCTGGGTGAGGTACTGGG‑TGGGCTGCCACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGATGAAGTCAAGGGCTTGATCCAGGCGCGCCTGACGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGAcc                                                                                                               <  8:60671/149‑1 (MQ=255)                                       tGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGATAAGAACTGGGCTGAGTCTGACCg                                                                                                              >  7:189331/1‑148 (MQ=255)                                             aTGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTcgc                                                                                                       <  1:98755/149‑1 (MQ=255)                                                tACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGAt                                                                                                    <  1:239759/149‑1 (MQ=255)                                                  cTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCa                                                                                                  <  6:35481/149‑1 (MQ=255)                                                     gg‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCt                                                                                               >  7:61219/1‑149 (MQ=255)                                                     gg‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCt                                                                                               >  3:143871/1‑149 (MQ=255)                                                          tgctgcAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGAc                                                                                            <  4:61295/148‑1 (MQ=255)                                                                aaCTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATg                                                                                     <  4:59305/149‑1 (MQ=255)                                                                          cGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGc                                                                           <  8:204432/149‑1 (MQ=255)                                                                          cGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGc                                                                           >  8:228880/1‑149 (MQ=255)                                                                               aCTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTgga                                                                       <  5:72443/148‑1 (MQ=255)                                                                               aCTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTgga                                                                       <  8:196384/148‑1 (MQ=255)                                                                                 ttCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTggagga                                                                    >  5:247164/1‑149 (MQ=255)                                                                                 ttCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTggagga                                                                    >  3:286477/1‑149 (MQ=255)                                                                                 ttCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTggagga                                                                    >  6:239066/1‑149 (MQ=255)                                                                                 ttCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTggagga                                                                    >  3:258225/1‑149 (MQ=255)                                                                                    cTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACa                                                                  <  8:61219/148‑1 (MQ=255)                                                                                        tctcTGGTTCAGAAGGAAAGGTCTATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCaa                                                              <  8:256327/145‑1 (MQ=255)                                                                                        gTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGATAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAg                                                             <  8:189331/149‑1 (MQ=255)                                                                                        gTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAg                                                             >  3:35224/1‑149 (MQ=255)                                                                                          gCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAggg                                                           <  4:258225/149‑1 (MQ=255)                                                                                           cTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAggg                                                           >  4:89854/1‑148 (MQ=255)                                                                                           cTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGc                                                          <  5:239066/149‑1 (MQ=255)                                                                                            tGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAgaga                                                          >  7:256327/1‑145 (MQ=255)                                                                                            tGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCa                                                         >  1:238008/1‑149 (MQ=255)                                                                                              gTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACAGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCAc                                                        >  8:83971/1‑148 (MQ=255)                                                                                               tGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGa                                                      <  4:35224/149‑1 (MQ=255)                                                                                                    aaGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTAcc                                                 >  8:261487/1‑149 (MQ=255)                                                                                                        cAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATgg                                                                                    >  1:11414/1‑110 (MQ=255)                                                                                                        cAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATgg                                                                                    <  2:11414/110‑1 (MQ=255)                                                                                                        cAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGc                                             <  7:83971/149‑1 (MQ=255)                                                                                                         aaGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCg                                            >  2:59434/1‑149 (MQ=255)                                                                                                                aTGCGGCTGAAGTCGAGTGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGGCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTAGCTGGCGTCTGGct                                     <  2:368481/149‑1 (MQ=255)                                                                                                                           gTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTaa                          >  7:291247/1‑149 (MQ=255)                                                                                                                            tCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGaa                              <  1:73120/144‑1 (MQ=255)                                                                                                                            tCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGaa                              >  2:73120/1‑144 (MQ=255)                                                                                                                            tCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAAc                         >  2:336291/1‑149 (MQ=255)                                                                                                                               aGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTgg                      <  2:55015/149‑1 (MQ=255)                                                                                                                                  gCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGc                   <  7:228880/149‑1 (MQ=255)                                                                                                                                                   cTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGc  <  8:291247/149‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GGAGGCCGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGC  >  NC_002947/3304483‑3304776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑