Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,304,631 A→C 7.7% K423Q (AAG→CAG)  cysS → cysteine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,304,6310AC 7.7% 200.0 / 5.4 65K423Q (AAG→CAG) cysScysteine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (28/32);  new base C (2/3);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

AGGCCGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTT  >  NC_002947/3304485‑3304778
                                                                                                                                                   |                                                                                                                                                   
aGGCCGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAg                                                                                                                                                   <  3:63074/149‑1 (MQ=255)
    cGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGgcgc                                                                                                                                               >  7:229094/1‑149 (MQ=255)
    cGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGgcgc                                                                                                                                               <  6:26625/149‑1 (MQ=255)
    cGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGgcgc                                                                                                                                               >  3:61295/1‑149 (MQ=255)
     gctgctGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGcgcg                                                                                                                                              >  2:239759/1‑149 (MQ=255)
      ctgctgGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTTCAGGcgcgc                                                                                                                                             <  6:176881/149‑2 (MQ=255)
       tgctgGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTTCAGGcgcgcc                                                                                                                                            >  6:235866/1‑147 (MQ=255)
       tgctgGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTTCAGGcgcgcc                                                                                                                                            <  5:241282/149‑3 (MQ=255)
       tgctgGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCGTCAGGcgcgcc                                                                                                                                            >  6:241282/1‑147 (MQ=255)
         ctggcctggcCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTTCAGGcgcgcc                                                                                                                                            <  5:235866/147‑3 (MQ=255)
         ctggcctggcCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCg                                                                                                                                          >  1:234793/1‑149 (MQ=255)
         ctggcctggcCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCg                                                                                                                                          >  2:98755/1‑149 (MQ=255)
          tggactggccGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCg                                                                                                                                          >  1:368481/1‑148 (MQ=255)
              ctggccGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACaa                                                                                                                                     <  8:229094/149‑1 (MQ=255)
                 gccGGTCGACTGCGCGAGCTGGGTGATGTACTGGGATGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCGTGAAGGCGCGTGCGGACAAg                                                                                                                                    >  1:55015/1‑148 (MQ=255)
                 gccGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAg                                                                                                                                    <  2:234793/147‑1 (MQ=255)
                    ggTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTg                                                                                                                               >  7:276370/1‑149 (MQ=255)
                      tCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGGGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTggg                                                                                                                             <  1:365900/149‑1 (MQ=255)
                        gACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGc                                                                                                                            <  7:61150/148‑1 (MQ=255)
                        gACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCt                                                                                                                           <  2:18701/149‑1 (MQ=255)
                          cTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGa                                                                                                                         <  8:39604/149‑1 (MQ=255)
                           tGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAg                                                                                                                        >  7:196384/1‑149 (MQ=255)
                                gAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGa                                                                                                                   <  2:98868/149‑1 (MQ=255)
                                gAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGa                                                                                                                   <  8:52924/149‑1 (MQ=255)
                                  gCTGGGTGAGGTACTGGG‑TGGGCTGCCACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGATGAAGTCAAGGGCTTGATCCAGGCGCGCCTGACGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGAcc                                                                                                                 <  8:60671/149‑1 (MQ=255)
                                    tGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGATAAGAACTGGGCTGAGTCTGACCg                                                                                                                >  7:189331/1‑148 (MQ=255)
                                          aTGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTcgc                                                                                                         <  1:98755/149‑1 (MQ=255)
                                             tACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGAt                                                                                                      <  1:239759/149‑1 (MQ=255)
                                               cTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCa                                                                                                    <  6:35481/149‑1 (MQ=255)
                                                  gg‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCt                                                                                                 >  3:143871/1‑149 (MQ=255)
                                                  gg‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCt                                                                                                 >  7:61219/1‑149 (MQ=255)
                                                       tgctgcAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGAc                                                                                              <  4:61295/148‑1 (MQ=255)
                                                             aaCTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATg                                                                                       <  4:59305/149‑1 (MQ=255)
                                                                       cGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGc                                                                             <  8:204432/149‑1 (MQ=255)
                                                                       cGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGc                                                                             >  8:228880/1‑149 (MQ=255)
                                                                            aCTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTgga                                                                         <  8:196384/148‑1 (MQ=255)
                                                                            aCTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTgga                                                                         <  5:72443/148‑1 (MQ=255)
                                                                              ttCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTggagga                                                                      >  6:239066/1‑149 (MQ=255)
                                                                              ttCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTggagga                                                                      >  3:286477/1‑149 (MQ=255)
                                                                              ttCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTggagga                                                                      >  5:247164/1‑149 (MQ=255)
                                                                              ttCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTggagga                                                                      >  3:258225/1‑149 (MQ=255)
                                                                                 cTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACa                                                                    <  8:61219/148‑1 (MQ=255)
                                                                                     tctcTGGTTCAGAAGGAAAGGTCTATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCaa                                                                <  8:256327/145‑1 (MQ=255)
                                                                                     gTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGATAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAg                                                               <  8:189331/149‑1 (MQ=255)
                                                                                     gTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAg                                                               >  3:35224/1‑149 (MQ=255)
                                                                                       gCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAggg                                                             <  4:258225/149‑1 (MQ=255)
                                                                                        cTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAggg                                                             >  4:89854/1‑148 (MQ=255)
                                                                                        cTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGc                                                            <  5:239066/149‑1 (MQ=255)
                                                                                         tGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAgaga                                                            >  7:256327/1‑145 (MQ=255)
                                                                                         tGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCa                                                           >  1:238008/1‑149 (MQ=255)
                                                                                           gTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACAGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCAc                                                          >  8:83971/1‑148 (MQ=255)
                                                                                            tGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGa                                                        <  4:35224/149‑1 (MQ=255)
                                                                                                 aaGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTAcc                                                   >  8:261487/1‑149 (MQ=255)
                                                                                                     cAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATgg                                                                                      >  1:11414/1‑110 (MQ=255)
                                                                                                     cAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATgg                                                                                      <  2:11414/110‑1 (MQ=255)
                                                                                                     cAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGc                                               <  7:83971/149‑1 (MQ=255)
                                                                                                      aaGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCg                                              >  2:59434/1‑149 (MQ=255)
                                                                                                             aTGCGGCTGAAGTCGAGTGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGGCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTAGCTGGCGTCTGGct                                       <  2:368481/149‑1 (MQ=255)
                                                                                                                        gTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTaa                            >  7:291247/1‑149 (MQ=255)
                                                                                                                         tCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGaa                                >  2:73120/1‑144 (MQ=255)
                                                                                                                         tCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGaa                                <  1:73120/144‑1 (MQ=255)
                                                                                                                         tCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAAc                           >  2:336291/1‑149 (MQ=255)
                                                                                                                            aGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTgg                        <  2:55015/149‑1 (MQ=255)
                                                                                                                               gCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGc                     <  7:228880/149‑1 (MQ=255)
                                                                                                                                                cTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGc    <  8:291247/149‑1 (MQ=255)
                                                                                                                                                   aaGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCtt  <  4:286477/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AGGCCGCTGCTGGCCTGGCCGGTCGACTGCGCGAGCTGGGTGATGTACTGGG‑TGTGCTGCAACTGGAGGCCGATGACTTCCTGCGTGCTGGTGCCGAAGGCAAGGTCGATGCGGCTGAAGTCGAGGGCTTGATCCAGGCGCGCCTGAAGGCGCGTGCGGACAAGAACTGGGCTGAGTCTGACCGCATTCGCGATCAGCTGACCGCCATGGGTGTAGTGCTGGAGGACAGCAAGGGCACGACTACCTGGCGTCTGGCTGACTGAAGTAACTGGGGCTGCACCGCAGCCCCAGCTT  >  NC_002947/3304485‑3304778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑