Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,352,174 A→G 11.1% intergenic (+64/+78) PP_2947 → / ← PP_2948 transcriptional regulator MvaT/GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,352,1740AG11.1% 117.9 / 5.6 45intergenic (+64/+78)PP_2947/PP_2948transcriptional regulator MvaT/GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (21/19);  new base G (2/3);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

CAAGGGTGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTGGGCGACATTGAAACGGA  >  NC_002947/3352032‑3352322
                                                                                                                                              |                                                                                                                                                    
cAAGGGTGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCg                                                                                                                                                >  6:202659/1‑149 (MQ=255)
      tGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACGCTGTCGcttcct                                                                                                                                          <  6:197047/149‑1 (MQ=255)
      tGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACGCTGTCGcttcct                                                                                                                                          <  6:202090/149‑1 (MQ=255)
      tGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGcctcct                                                                                                                                          <  2:53353/149‑1 (MQ=255)
        gCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTGTTTTTCGAGGAAGCGACAGCGTCGCctcctc                                                                                                                                         <  1:38266/148‑1 (MQ=255)
              acaAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAg                                                                                                                                  >  2:358170/1‑149 (MQ=255)
                              aTGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGGGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGGCGCCTCCTCGAAACAGGGCGTTCATCgtgggt                                                                                                                  >  6:243558/1‑149 (MQ=255)
                                      cccAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGaa                                                                                                          >  5:327255/1‑149 (MQ=255)
                                      cccAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGaa                                                                                                          >  1:278280/1‑149 (MQ=255)
                                       ccAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAAt                                                                                                         <  5:348451/149‑1 (MQ=255)
                                          gTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAg                                                                                                      <  2:233445/149‑1 (MQ=255)
                                           tACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGc                                                                                                     <  1:192762/149‑1 (MQ=255)
                                           tACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGc                                                                                                     <  4:107370/149‑1 (MQ=255)
                                                      agaagTGGAATCCTGGGTGTCCTGATTCGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGtt                                                                                           <  7:354983/148‑1 (MQ=255)
                                                         agTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATCGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAAc                                                                                       <  7:212468/149‑1 (MQ=255)
                                                          gTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACa                                                                                      <  1:358170/149‑1 (MQ=255)
                                                            ggAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAg                                                                                     >  6:366664/1‑148 (MQ=255)
                                                                 gcTGGGTGTCCTGATTTGTGCTGATCTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGTCGGAATGAGATGACGTGGTTAAACAGGAAGtt                                                                               <  3:49477/148‑1 (MQ=255)
                                                                         tCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCAc                                                                        <  6:327255/148‑1 (MQ=255)
                                                                            tGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAg                                                                    >  3:138306/1‑149 (MQ=255)
                                                                              aTTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACGGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAgcg                                                                  >  5:217330/1‑149 (MQ=255)
                                                                                     cTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAgt                                                            <  5:88580/148‑1 (MQ=255)
                                                                                     cTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCACTCATCATGGCGTTCATCGTGGGTGGGCGGATAGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGGGCTCAgag                                                           >  4:221848/1‑147 (MQ=255)
                                                                                      tGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAgtgt                                                          >  1:155584/1‑149 (MQ=255)
                                                                                             gCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGttt                                                    <  1:190896/148‑1 (MQ=255)
                                                                                                   aTTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCa                                                                         >  7:294607/1‑121 (MQ=255)
                                                                                                   aTTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCa                                                                         <  8:294607/121‑1 (MQ=255)
                                                                                                        gcTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGt                                         >  6:177393/1‑148 (MQ=255)
                                                                                                           aTCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAAt                                     >  2:17966/1‑149 (MQ=255)
                                                                                                            tCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAAtt                                    >  8:202483/1‑149 (MQ=255)
                                                                                                             cTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAAttt                                   >  7:199174/1‑149 (MQ=255)
                                                                                                              tGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGttt                                                    <  1:212730/131‑1 (MQ=255)
                                                                                                              tGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGttt                                                    >  2:212730/1‑131 (MQ=255)
                                                                                                              tGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTCGGCGGAATGAGCTGACGTTGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAAAACTTCGTAAAtttt                                  >  8:2556/1‑149 (MQ=255)
                                                                                                                  aCGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCa                              >  3:42973/1‑149 (MQ=255)
                                                                                                                      cTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAg                          <  5:202659/149‑1 (MQ=255)
                                                                                                                      cTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAg                          >  7:46887/1‑149 (MQ=255)
                                                                                                                       ttGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGc                         >  4:34700/1‑149 (MQ=255)
                                                                                                                          ccctgtttCGAGGAGGCGACGCTGTCGCTTCCTCTAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGa                      >  5:317657/6‑149 (MQ=38)
                                                                                                                           tCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGAt                     >  3:364926/1‑149 (MQ=255)
                                                                                                                               tttCGAGGAAGCGACGGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTgg                  <  6:217330/148‑1 (MQ=255)
                                                                                                                               tttCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCTGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTgg                  <  4:56249/148‑1 (MQ=255)
                                                                                                                                 tCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTGGGc                <  5:366664/148‑1 (MQ=255)
                                                                                                                                         gCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTGGGCGACATTGaa       <  5:177393/149‑1 (MQ=255)
                                                                                                                                         gCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTGGGCGACATTGa        >  1:144472/1‑148 (MQ=255)
                                                                                                                                              aGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTGGGCGACATTGAAACgga  <  2:278280/149‑1 (MQ=255)
                                                                                                                                              aGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTGGGCGACATTGAAACgga  <  5:243558/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                    
CAAGGGTGGCAACCACAAGCTGCTCAAGGAATGGAAAGCCCAGTACGGGGCAGAAGAAGTGGAATCCTGGGTGTCCTGATTTGTGCTGATGTCGCCCTCATTGCGCTATCTGAAACGCCTTGTTCTTTTTCGAGGAAGCGACAGCGTCGCCTCCTCGAAACAGGGCGTTCATCGTGGGTGGGCGGAATGAGCTGACGTGGTTAAACAGGAAGTTTCGCCACTAAGCGCTCAGTGTCCGTTTCAATACTTCGTAAATTTTGGCATCAGCCGATTGGGCGACATTGAAACGGA  >  NC_002947/3352032‑3352322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑