Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,423,932 G→A 7.8% R697Q (CGG→CAG)  PP_3038 → TraC domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,423,9320GA 7.8% 142.7 / 2.7 51R697Q (CGG→CAG) PP_3038TraC domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (22/25);  new base A (2/2);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.72e-01

GATCCTGAAATGGTTGGAGGAATCCCAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAACACATAGATGAGATGCTGA  >  NC_002947/3423786‑3424077
                                                                                                                                                  |                                                                                                                                                   
gATCCTGAAATGGTTGGAGGAATCCCAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGAGGCGGGTGGGATCGATCATGCACCGCCTAGGTTggcggc                                                                                                                                                   <  3:326053/149‑1 (MQ=255)
     tGAAATGGTTGGAGGAATCCCAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCg                                                                                                                                               >  1:286777/1‑148 (MQ=255)
       aaaTGGTTGGAGGAATCCCAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAg                                                                                                                                            >  4:10934/1‑149 (MQ=255)
          tGGTTGGAGGAATCCCAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAggct                                                                                                                                         <  4:92930/149‑1 (MQ=255)
          tGGTTGGAGGAATCCCAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAggct                                                                                                                                         <  1:167043/149‑1 (MQ=255)
            gTTGGAGGAATCCCAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTGGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAagctg                                                                                                                                        <  4:42734/148‑1 (MQ=255)
                 aggaATCCCAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCAGCGTCGCAGGCTGgctgcg                                                                                                                                  >  2:112331/1‑149 (MQ=255)
                 aggaATCCCAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCAGCGTCGCAGGCTGgctgcg                                                                                                                                  >  2:117007/1‑149 (MQ=255)
                         cAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGaa                                                                                                                        <  2:133931/149‑1 (MQ=255)
                         cAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGaa                                                                                                                        <  1:337117/149‑1 (MQ=255)
                         cAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTAGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑Cg                                                                                                                          >  3:358941/1‑147 (MQ=255)
                              cGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCg                                                                                                                   >  1:55506/1‑149 (MQ=255)
                                     aCGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAgcg                                                                                                            <  3:183774/149‑1 (MQ=255)
                                         tCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCt                                                                                                        <  5:220669/149‑1 (MQ=255)
                                           aCCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCtt                                                                                                       <  4:233574/148‑1 (MQ=255)
                                             cGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAaga                                                                                                                      <  4:204887/131‑1 (MQ=255)
                                             cGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAaga                                                                                                                      >  3:204887/1‑131 (MQ=255)
                                                   cGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCatat                                                                                               >  3:322607/1‑148 (MQ=255)
                                                                 aggcaTTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGt                                                                                >  3:236710/1‑149 (MQ=255)
                                                                  ggcaTTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGtt                                                                               >  8:311066/1‑149 (MQ=255)
                                                                      ttGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTgggg                                                                           <  4:236710/149‑1 (MQ=255)
                                                                         aaCCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGgcg                                                                        >  5:311332/1‑149 (MQ=255)
                                                                          aCCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGgcg                                                                        >  4:32767/1‑148 (MQ=255)
                                                                           ccTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGgcg                                                                        >  7:90543/1‑147 (MQ=255)
                                                                            cTTGACCCTGGACACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGTTAAGCGCCATTGGGCATATCAGAAGCATGATGGTTGGGGCCGCAg                                                                     >  7:321470/1‑149 (MQ=255)
                                                                                 cccTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGTATCGATCATTCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGcc                                                                 <  8:321470/148‑1 (MQ=255)
                                                                                   cTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTg                                                              <  7:230919/149‑1 (MQ=255)
                                                                                   cTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCACAGCGCCTTg                                                              <  2:244291/149‑1 (MQ=255)
                                                                                      gCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGa                                                            <  6:311332/148‑1 (MQ=255)
                                                                                         aCTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAg                                                        >  3:5764/1‑149 (MQ=255)
                                                                                          cTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCAGCGTCGCAGGCTGGCTGCGCTGCTGCGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCa                                                         <  1:112331/149‑1 (MQ=255)
                                                                                          cTGGGGCAAGCCTGAGCAGATGCGGGTGGGATAGATCAATCACCGCCTATGTTGGCAGCGTCGCAGGCTGGCTGCGCTGCGAGGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCa                                                         <  1:117007/149‑1 (MQ=255)
                                                                                             gggCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCa                                                    <  3:10934/149‑1 (MQ=255)
                                                                                                 aaGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCa                                                <  4:322607/149‑1 (MQ=255)
                                                                                                    ccTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGcc                                             <  3:71484/149‑1 (MQ=255)
                                                                                                             aTGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAaggag                                      >  6:318037/1‑147 (MQ=255)
                                                                                                                  ggTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCCCGCAGCCGAAGGAGGAGTGCt                               >  7:261301/1‑149 (MQ=255)
                                                                                                                         tcgatcATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATc                        <  5:318037/149‑1 (MQ=255)
                                                                                                                           gatcATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCtttt                            <  7:297491/143‑1 (MQ=255)
                                                                                                                           gatcATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCtttt                            >  8:297491/1‑143 (MQ=255)
                                                                                                                            atcATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGTGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCaaa                     <  7:311066/149‑1 (MQ=255)
                                                                                                                             tcATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAac                    <  5:97839/149‑1 (MQ=255)
                                                                                                                                tGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAAcaca                 >  8:326237/1‑149 (MQ=255)
                                                                                                                                  cACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAAcaca                 >  6:211268/1‑147 (MQ=255)
                                                                                                                                   aCCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAAcaca                 >  3:86567/1‑146 (MQ=255)
                                                                                                                                   aCCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAAcaca                 >  1:159672/1‑146 (MQ=255)
                                                                                                                                    ccGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAACACATaga             <  2:159672/149‑1 (MQ=255)
                                                                                                                                      gCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGAAGGAGTGCTTTTGATCAAACACATagat            >  8:202559/1‑148 (MQ=255)
                                                                                                                                       ccTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAACACATagatga          <  8:90543/149‑1 (MQ=255)
                                                                                                                                         tAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAACACATagat            >  8:52658/1‑145 (MQ=255)
                                                                                                                                           ggTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGAAGGAGTGCTTTTGATCAAACACATagatgaga        <  7:202559/147‑1 (MQ=255)
                                                                                                                                            gTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAACACATagatgagatg      <  7:52658/148‑1 (MQ=255)
                                                                                                                                                gcggcgTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAACACATAGATGAGATGCTGa  >  7:225344/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GATCCTGAAATGGTTGGAGGAATCCCAGGCCGGCGAGACGGTCACCGGAAGCGAAGTGTTGGGGCAGGCATTGAACCTTGACCCTGGCCACTGGGGCAAGCCTGAGCAGATGCGGGTGGGATCGATCATGCACCGCCTAGGTTGGCGGCGTCGCAGGCTGGCTGCGCTGC‑‑CGAAGAGCGGTAAGCGCCCTTGGGCATATCAGAAGCCTGATGGTTGGGGGCGCAGCGCCTTGGAGCAGTCCACGCAGCCGAAGGAGGAGTGCTTTTGATCAAACACATAGATGAGATGCTGA  >  NC_002947/3423786‑3424077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑