| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,424,890 | C→G | 8.6% | intergenic (+478/‑34) | PP_3039 → / → PP_3040 | hypothetical protein/holin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,424,890 | 0 | C | G | 8.6% | 166.8 / 5.0 | 56 | intergenic (+478/‑34) | PP_3039/PP_3040 | hypothetical protein/holin |
| Reads supporting (aligned to +/- strand): ref base C (27/24); new base G (2/3); total (29/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
ATTCAACAGACATTCCCCGGACGTTTACCACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGACATGGCCGGCGT > NC_002947/3424742‑3425033 | aTTCAACAGACATTCCCCGGACGTTTACCACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGcc > 7:126782/1‑149 (MQ=255)aTTCAACAGACATTCCCCGGACGTTTACCACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGcc < 7:241916/149‑1 (MQ=255) aaCAGACATTCCCCGGACGTTTACCACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTgg > 2:116867/1‑149 (MQ=255) aCAGACATTCCCCGGACGTTTACCACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTACGAGACGTGCGCGCGCACAAACCGGCCCGCCACACACT‑GAAAACCCCGGCCCTGgc > 2:348716/1‑149 (MQ=255) ccccGGACGTTTACCACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCGCCAGGGCCGGGGt < 3:206912/148‑1 (MQ=255) ccGGACGTTTACCACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCGCCAGGGCCGGGGtttt < 3:33527/149‑1 (MQ=255) ccGCACGTTTACCACTTATTCCCGGGTGGCATTAAAACTCCCTTCCTGCCACCGGAATCCACCTCTAAAAACTACCCATCTTCGAGCCGTGCGGGCGCACAAACCCGCCCGCCAAACACT‑CAAAAAGCCGGCCCTGGCGCCGGGGtttt < 7:57740/149‑1 (MQ=255) ggACGTTTACCACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTA‑CCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGc > 7:336497/1‑149 (MQ=255) gACGTTTACCACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACTGGAAAACCCCGGCGCCAGGGCCGGGGttttca < 1:34634/149‑3 (MQ=255) ccACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAACCCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTggg < 2:108020/148‑1 (MQ=255) ccACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTggg < 2:240443/148‑1 (MQ=255) cACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTggggg > 5:33017/1‑149 (MQ=255) ttATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCgggg < 2:109818/130‑1 (MQ=255) ttATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCgggg > 1:109818/1‑130 (MQ=255) gCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGAt < 4:297672/148‑1 (MQ=255) ggTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACa < 7:65584/149‑1 (MQ=255) tGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGc > 8:176271/1‑149 (MQ=255) gCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGa < 2:95648/149‑1 (MQ=255) aTTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGag > 6:109944/1‑148 (MQ=255) aTTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGag > 7:93332/1‑148 (MQ=255) tAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGagcag > 6:126677/1‑149 (MQ=255) aaaCTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGagcagc < 2:341670/148‑1 (MQ=255) aaaCTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTA‑CCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCaa < 8:336497/149‑1 (MQ=255) aaCTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCGGGCGCCGGGGTTTTTGCCTAAGGGGGATTCGATGAAAAGCGagcagc > 6:300806/1‑147 (MQ=255) gCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGtt > 3:179588/1‑149 (MQ=255) cTTGCTGCCACCGGAATCGACCTGTAAAAACTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTa < 1:85847/149‑1 (MQ=255) gcCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCAGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGtt > 1:197970/1‑146 (MQ=255) cACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCCCCAAACACT‑TAAAACCCCGGCCCTGGCGCCCGGGTTTTTGGCAGTGGGGGGATGGGTGAACAGCGAGCCGCACAGGTTAGATgagct > 8:305548/1‑147 (MQ=255) gAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCaa > 1:343021/1‑149 (MQ=255) gACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCtgg < 5:126677/149‑1 (MQ=255) ccTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCtggtt < 7:176271/149‑1 (MQ=255) ccTGTAAAAAGTACCCATCATCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGTTGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCtggtt < 2:285076/149‑1 (MQ=255) cTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCtggttg > 4:283564/1‑149 (MQ=255) tAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACCCT‑GAAAACCCCGGCCCGGGCGCCGGGGGTTTTGGCTTTGGGGGAt > 2:192386/1‑98 (MQ=255) tAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGAt < 1:192386/98‑1 (MQ=255) tAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCtggttggt < 8:93332/148‑1 (MQ=255) aaaGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATc < 8:210162/149‑1 (MQ=255) aaaGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATc < 8:126782/149‑1 (MQ=255) gTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTc < 8:220889/149‑1 (MQ=255) tCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTccc > 5:15282/1‑149 (MQ=255) gagaCGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGt < 5:109944/148‑1 (MQ=255) gagaCGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTc < 4:179588/149‑1 (MQ=255) gaCGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTcgg > 3:142519/1‑149 (MQ=255) gggCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGgtg > 3:98186/1‑149 (MQ=255) gggCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGgtg > 3:92978/1‑149 (MQ=255) acaAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCGCTGGCGGCGGGGTTTTTGGCGTTGGGGGATTCGTTGAACAGCGAGCAGCATGGGTTAGTTGAGATGGGAATCTGGTTGGTGGTGTTCGTGTCGCTGGTCGGCGGGGTGTGAGGc > 8:115963/1‑149 (MQ=255) aaaGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCATGCGTTAGTGGAGAGGGCAATCTGGTTGGTGATGTTGCTGTCGCGGGGGGGGGGGGGGTCGGGc > 8:250969/1‑147 (MQ=255) aaaGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCATTCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGc > 5:110543/1‑147 (MQ=255) ccGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGc < 6:33017/149‑1 (MQ=255) aggcaaaaaCCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGAc > 5:122379/6‑149 (MQ=255) t‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGACATg > 1:94861/1‑149 (MQ=255) aaaaCCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGACATGGc > 5:30947/1‑149 (MQ=255) aaaaCCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGACATGGc > 3:177956/1‑149 (MQ=255) aaaaCCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCGGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGACATg < 3:283564/147‑1 (MQ=255) ccccGGCGCCAGGGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGGCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGACATGGCCGGc > 8:226316/1‑149 (MQ=255) cccGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGACATGGCCGGCg > 7:193987/1‑149 (MQ=255) ccGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGACATGGCCGGCGt < 2:94861/149‑1 (MQ=255) ccGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGACATGGCCGGCGt < 6:15282/149‑1 (MQ=255) | ATTCAACAGACATTCCCCGGACGTTTACCACTTATTGCCGGGTGGCATTAAAACTCGCTTGCTGCCACCGGAATCGACCTGTAAAAAGTACCCATCTTCGAGACGTGCGGGCGCACAAAGCGGCCCGCCAAACACT‑GAAAACCCCGGCCCTGGCGCCGGGGTTTTTGCCTTTGGGGGATTCGATGAACAGCGAGCAGCAAGCGTTAGTTGAGATGCCAATCTGGTTGGTGATCTTCCTGTCCCTGGTCGGCGGGGTGTCAGGCGAGATGTGGCGGGCCGACATGGCCGGCGT > NC_002947/3424742‑3425033 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |